DNA Double Strand Breaks And Repair Systems Part 2
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- čas přidán 7. 08. 2024
- This Video Explains The DNA Double Strand Breaks And Homologous Recombination (HR) Repair System Versus Non-homologous End-Joining (NHEJ).
Link For The First Part Of This Video:
• DNA Repair Mechanisms ...
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It's really a great video and so clear explanation... This channel deserves to be followed and subscribed.. I hope you will make new videos always in genetics, genetic engineering, gene therapy and so on , thank you so much.
Thank you. We will cover all major topics of genetics as well 😀
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I.
My uni lecturer had me thinking this was impossible to understand, you’ve made it so clear and simple in 11 minutes, thank you som ugh for all the work you put into these.
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Great job! As a CZcamsr myself, I can really appreciate the amount of work that went into this haha
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Actually i am a bit confused before watching your video as I studied from my coaching notes 😅 but after watched your video I feel blessed. You make this concept so easy peasy ❤️
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Great video! to the point and clear
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Thank you for speaking slowly and slowing down your diagrams! I think this helped
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Amazing explanation and Presentation....Understood the content easy peasy.... Thank you!❤👏👏👏🥰
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That was an amazing lecture. Thank you so much
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I'm learned a lot from your work, thnx for sharing sister 🙌🏽💚
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thank you, that was great work helped me alot.
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I am previously badly Stuck with this point but now its Crystal cleared Alhamdulillah.
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Thank you very much. I love your accent!
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Thank u so much ..for simplyfying the concept...👍
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Thank you, this video was easier to understand than my professor
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Very clearly explained by you...Thank u so much
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Thank you so much! It was so helpful
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Thanks a lot, its very clear explanation.
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it's really awesome I hope you translate it to another languages it's gonna be helpful for a lot of people👏🏻👏🏻
Thank you 😊. We will try to work on your suggestion 😊
Very helpful video. Very good efforts mam
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Perfectly explained this concept thanks alot
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appreciation!!!!!!! tremendous appreciatioN!!
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Amazing explanation.... Thnks alot
Thanks alot for your support. It means alot.
The first VDO that I understand thxx a lot
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Some of us have a difficult time understanding different accents but your English is very clear and almost every word was easy for me to understand. I've lost some of my hearing and had a difficult time hearing the word you used @1:14. I have little to no experience with DNA and more than likely the reason I did not understand that word.
Your work is obviously helping others to understand better. I hope you continue to teach as you are very good at this. ❤
Thank you Priscilla 😊, the word was lesion that is an area of abnormal tissue. English is not my first language, maybe I pronounced it wrong. Thank you for your support 🙏
@@EasyPeasyLearning OK I get it now and thank you for responding.
The word sounds like "le´zhun" according to dictionaries. English is my only language but I often get words wrong especially when it comes to the sciences. You've done a lot better than I would have!
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Concept clear 🙏 Thank you
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Concept clear thank U 🙏
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Good explanation Like Always
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Awesome
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Very informative and understandable. Thank you.
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thank you so much, I really understand it. It was so clear
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Nice video ma'am ❤
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How often does dna double strand breaks in normal days (not by medical radiation/xray/ct scan)? How to know if a ct scan double strand break has been repaired correctly?
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Thank you for the detailed explanation. Just a query, from the cell's viewpoint, why not use homologous recombination as far as possible if it so less prone to loss of DNA? What would make a cell choose NHEJ?
Hi Prabal, in non-homologous pathway the part of DNA that can still be use as template will first follow the homologous recombination pathway but if the strand have mutations and can not be use as a template then it will shift itself on the non homologous pathway.
@@EasyPeasyLearning Hmm, that surely makes sense. Thanks a lot for clarifying.
homologous recombination is more used by bacteria. NHEJ is the most used by mammals
Excellent... Easy to understand thankyou easy peasy....
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Brilliant video... Is there any reference for the Homologous repair for me to read further??
TIA😊
Thank you for your comment, these are different sites to have more insight about the topic
www.nature.com/articles/cr20081
blog.addgene.org/crispr-101-homology-directed-repair
For the cross over HR repair, if one part of a homologous chromosome is used for the damaged chromosome, won’t the original one be missing parts?
This a great explanation thanks but really worrying having had 6 CT abdominal CT scan within 3 years due to digestive issues .... How could my DNA take that much of X ray and repair efficiently ? Really worried and will appreciate any answer
Hello Sami,
Unfortunately we are not medical professionals due to which we cannot comment or guide you on this matter. We hope you get well soon.
Hi, Thank you for the nice explanation. So if I understand correctly then HR is better than NHEJ? Because in NHEJ part of DNA is lost. Am I right?
Hi Pratik,
HR leads to accurate repair, while NHEJ is intrinsically mutagenic but NHEJ is a faster and more efficient DSB repair pathway than HR. So both pathways have there own benefits and disadvantages. The Reference Link for this statement is pubmed.ncbi.nlm.nih.gov/18675941/
Hey thank you for this amazing lecture
But I have a question
If the break was in the 5% part that is not similar in the homologous chromosome what's gona happen then ??????
Hi Belalia, that probably will lead to NHEJ pathway.
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how does DNA double strand break lead us to face lions? That was what narrator said before disability lines
So what is the big differrence? HR breaks and uses the other sister chromatid as a template. But why is this not an option for NHEJ?
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Really great video but please block these bots with NSFW profile picture
Thanks I was wondering why people are giving same type of comments. Thanks I appreciate it 🙏
Praise evolution
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You have used the term "double helix" at the start of each phenomenon. I think you meant "double strands"....
2.23
Makes me want to eat poutine.
lots of incorrect information oof
Hi Henry, all information provided on our videos is from books or proven articles. Which information do you think is provided incorrectly? We can look into the matter and correct if required.
@@EasyPeasyLearning By proven articles you mean peer-reviewed articles? a link to those would be excellent. Some information is misleading or not correct, for example, you mentioned DNA double-strand damage occurring primarily from radiation, of which the most common source is nuclear bombs.. source? I am sure that is not correct. The most well-known cause of DNA double breaks is radiation and chemotherapy, but also endogenous processes like replication. In addition to this, the common source of Ionizing radiation is radon gas or UV exposure, even some medical equipment... not a bomb. You never mention endogenous sources of DNA double-strand break coming from lymphocyte differentiation or replication errors. In addition to this, you mention Mre11 Rad50 and Nbs1 being just 3 proteins instead of being a heterodimer complex of M2R2N2. You also mention the 3' end resection overhang is 1000bp, and I would really like to see a source for that. In my experience end resection is highly variable.
In addition to this, Ku proteins and MRN complex can funciton in both NHEJ and HR. The pathway choice is dependent on environment and cell stage... along with a couple other factors.
Hi Henry! Thank you for the feedback. We will looked into the matter and asked regarding about the information you gave. The answers are given below:
Atomic bomb were suppose to be used as a reference of radiation here and should have not been mentioned as a primary cause. We will correct this information accordingly.
The UV radiations that you mentioned is not mentioned in this video because this video is a second part of our previous video. We will correct this mistake and rename the videos as part 1 and part 2 The link to the previous video is:
czcams.com/video/oRfK85gPAVA/video.html
The 3' overhang value was not mentioned as a solid fact but was rather intended to be given as an approximation. We will edit that portion to include approx symbol to clear the misunderstanding.
The ku proteins you mentioned are not included to keep the video short and simple.
Thank you for your time and feedback.
@@EasyPeasyLearning sounds excellent. thank you for getting back to me
Awesome
Thank you 😊