Adipose Stem Cells Generation by Mature Adipocytes Dedifferentiation | Protocol Preview

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Generation of Human Adipose Stem Cells through Dedifferentiation of Mature Adipocytes in Ceiling Cultures - a 2 minute Preview of the Experimental Protocol
Julie Lessard, Julie Anne Côté, Marc Lapointe, Mélissa Pelletier, Mélanie Nadeau, Simon Marceau, Laurent Biertho, André Tchernof
IUCPQ Research Center,; CHU de Québec Research Center,; Laval University,;
Mature adipocytes may represent an abundant source of stem cells through dedifferentiation, which leads to a homogenous population of fibroblast-like cells. Collagenase digestion is used to isolate mature adipocytes from human fat. The goal of our protocol is to obtain multipotent, dedifferentiated fat cells from human mature adipocytes.
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