Super Liquid Culture | Best Recipe for Mushroom Growing
Vložit
- čas přidán 8. 07. 2024
- Today I'll be showing you the best liquid culture recipe I've come across so far.
00:28 Intro
01:25 Ingredients Needed
2:20 How to Make LC
5:06 Pressure Cook at 15psi for 30 minutes
5:27 Final Results
6:16 Infogram / Outro
Links to ingredients in the video
Peptone: amzn.to/32zCcgR
Light malt extract: amzn.to/33OcvKb
Corn Syrup: amzn.to/3o4d3ST
Liquid Culture Lids: amzn.to/35vQdxj
⬙ PGT Links ⬙
🧫Links to all products/gear I use: kit.co/PhillyGoldenTeacher
(Using these links will help sensei earn a small commission and support the channel)
👕Merch Shop: teespring.com/stores/my-store...
📱Instagram: / phillygoldenteacher
📄Patreon: / phillygoldenteacher
💻Discord: / discord
⚪️Microppose Link for Injection Ports, use code PGT for a 10% discount.
microppose.com/r?id=r87sbd
💉Looking for Spores? Use code PGT for a discount here.
inoculatetheworld.com/?ref=24
🦠DrMyc Products
www.drmyc.com?sca_ref=1761105.0L3Ag7LnMw Use code PGT for a discount here.
🌿Fungstrate
fungstrateco.com/?ref=337 Use code PGT for 5% off subs and grains.
🌱BoomerShroomer Inflatable Tubs
boomershroomer.com/?ref=64 Use code PGT for discount here.
🍄MushroomSupplies
mushroom-supplies.sjv.io/5g5vr9 Use code PGT for 10% off.
Vivosun - Code PGT gets you 12% off.
bit.ly/3Fqa1BD - Jak na to + styl
600 ml H2O
12 ml / 18 g corn syrup
1/4 t / 1.2 g light malt extract
1/2 t / 1 g peptone
Stir to dissolve.
Add stir bar (or stone)
Cap jar and wrap lid in foil.
Pressure cook @ 15 psi for 30 minutes.
When completely cooled, inoculate.
(Took notes for myself, but also wanted to say thank you for the video!)
Thank you for writing that out.
How do you inoculate this mixture with mycelium? Can you use part of a fresh mushroom?
@@cyberbid not my video, but I use it to culture a LSS into mycelium, and then I put a sample on an agar plate to test for contam. If it grows clean, I go back to the liquid culture and set the mycelium to grain spawn.
You could use a piece of a fresh cap, but might have a higher risk of contamination. Good luck!!
🧑🌾
I wish they’d use cups, teaspoons, 1/4 teaspoon etc. instead of weight measurements. 12 ml, 18 grams…, How much is that?
This Super LC recipe along with the BB brown rice tek has yielded fastest jar colonization times for me. I have dime size growth in 8-10 hours, 50% (shake) at 4 days and ready for bulk at 10-12 days. I use PGT 🧬 and go straight to super LC. Thank you for the time you put into these sir.🍄👍
I'm glad the recipe helps! Seems you are doing really well with your journey. Keep up the great work! 😁
@@PhillyGoldenTeacherquick question about the 15 psi. I live at lower elevation and my pressure cooker for all recipes is 5 psi due to elevation. Should that also apply to using in making LC?
@@BeeCJ I'll chime in, just go for 15psi you should be good
It really is top notch ..... I haven't had anything take over 2 weeks to fully colonize, it just spreads like wildfire it's gorgeous
@@PhillyGoldenTeacherIs there any recipes for making LC without a pressure cooker? I have one that only reaches 5psi
Way to go, pgt! Really enjoy your videos, you're helping me learn the right way as fast as possible.
You're the man philly! Keep feeding us the knowledge. Much appreciated.
Great to see you back! Happy harvesting!
sensei your words inspire !
Thanks a lot Sensei!! I used your technique from this video and have got amazing results. I have super healthy liquid culture!! Keep up the great work 👍
Pgt taught me how! I’ve had much success thanks to this man. Cheers bro.
Glad to see a new video bro.
Keep up the good work!
You’re an amazing teacher and I love your video style & the effort you put in
Love the peptone, thanx❤
This lc recipe was the first time I ever got a lc to work. Not saying that others don’t work. But the way you explain and the order you do it, really just everything is awesome 🤙🏽🤙🏽🤙🏽🤙🏽🤙🏽
That's awesome, I'm glad you got it to work! 😄
Looking forward to trying this tasty recipe. Grazie mille sensei.
U the goat!!!! I followed this tutorial and meeeeen the grow is noticeable STRONG!!!!! THANK YOU !!!
😎🍄
your videos are really helpful.thank you🤘
Nice vid philly G!
happy new years broski
Thank you again PGT :)
Love it !! 🍄🍄🍄🍄
Simpel as always, big thanx!.
Thanx Philly GT!
Aloha! Thank you for always educating! I learned tons from your video! 🤙🏿
killing it this week
Just got a pc. So stoked that I can make everything myself now! Thank you for this video. It's so simple!
Can’t wait to whip some up !
Thanks for the info. Very concise and useful!
Loved it ❤️☺️☺️
Love Mossy and the amazing PGT!
🙏🍄
I haven’t changed up my LC recipe for over a year but today I am trying this one.
Is this your preferred recipe?
Thanks as always!
😁🍄
Best timingggg
today i found a jar in my lab fridge with over 1 year old mycelium-perfectly fine and strong..great stuff
I use organic brown sugar and it works good too. Great video!
I use light malt extract and insect frass. I might add fish hydrolysate (peptones) when making my next LC.
Thanks teach!!!!🙏🏻
Just in time! Modified some lids for LC today. Thanks for all your knowledge Golden🤙🏻🤙🏻mush mush
I'm looking into modified plastic lids for my first time making LC, but kind of confused about those red syringe filters. Are they used to suck out the LC into the syringes?
@@chicofromph33nix64 You use the self healing injection port. Syringe filter is for fae
Thank you bro 👍
Very good video, thank you.
Nice informative vid!
Thank you for all your work! About to start fruiting conditions on my first tub and it’s been smooth sailing so far!
How were they??
Ya how'd it go?
How smooth was it?
Thanks for the tutorials! I’ve been using this recipe, only using honey instead of corn syrup. Excellent growth!
thanks for the feedback. I bought peptone yesterday and it stinks. Is it suppose to smell?
@@michaelkreisel3573 good habit in a lab is DONT smell stuff.
How much quantity of honey you have been using?
@@michaelkreisel3573 yes
Nice thanks for the information
Thanks bro!
Thank you for your time,should incubation process be in the dark , or doesn’t really matter?
Been using peptone for about a year all because of Mossy Creek!!!
Thank you❤️
Thank you!!
🙏
I quickly grabbed a marker and wrote your recipe down.Thank you.Twas short and precise.I'll follow it to the letter.I dont have a pressure cooker yet so i tried with rain water and some honey and I used my favorite sugar substitute,organic Agave.Any comments on that?I believe it should work.Thanks again for your video.I liked and subscribed.PEACE.
Thx for this... i had growth from spore in 3days
I love how you say you are using quart jars, but then go onto measure 600ml and the other measuring cup is in milliliters too. Metric reigns supreme! Keep up the hard work!
Hey, thanks bro. Appreciated.
Excited to try your method, I have had 2 unsuccessful attempts at LC. Agar and prints have been so easy for me but this has evaded me. THANK YOU
do you share your prints?
Yes he does 😂 lol …. How’s the library comin along guys!? Spore trading / selling itself is not prohibb’d!! Trade away!
Ive been bingeing your content this week. What a happy surprise this morning
Your channel is a diamond in the rough
Another heater, fam. That's a good recipe
Hey PGT, what are your thoughts on liquid innoculant vs liquid culture?
Brother, thank you so much for making this. I am hyped about trying to grow my own Oyster mushrooms and more. Mushrooms are so dope and interesting.
Props, peace, and blessings to you and yours in the name of The Lord Jesus Christ. amen
I had oyster mushroom culture on a petri dish for longer than 2 years in the fridge abd it still worjed perfectly. So in liquid culture it should last forever basically.
Mossy creek is the man
nice, thank you
😁
I seem to have done almost the same research you did and went about things very similarly. However I used dry corn sugar, the four pound bag, and made a pre-mix with the dry malt extract. I also cooked for 40 minutes because I figured a darker solution would be good for the mycelium, but it certainly is harder to see what is going on.
And it failed im sure. Utter clown.
Great video! Any tips on what to look for in terms of contams?
I use the same recipe but I like to add a pinch of calcium as well
Man these videos have taught me everything . Is this LC good for all mushrooms ?
Oh and thanks for your content mush appreciated😆
🙏🍄
great video
🙏
Thanks a ton for your videos. You helped me tremendously when I was starting out and I still check in to compare notes. What’s the deal with peptone? I’ve been incorporating hydrogen peroxide a bit to reduce contam, but what’s your experience with peptone?
put 10cc of the thick solution as a jump-start and then put constant stirring on for 24-48 hours, it will look almost entirely colonised
The liquid malt extract mixes and works well also .
Thanks for all the knowledge 🔥🌲🔥🍄✌🔥
Thank you
His got lots of good Stuff like, Psilocybin, Psychedelics, also have Shrooms, Spores Syringe, Micro-dose, Dmt, Lsd, Valium, Modafinil, XTC, Xanax, Grow kits, Grains, 1P-LSD, AL-LAD and lots more. He could guide you through anything you need?????
Feelgrabz...,,,
Reach him via his Instagram handle or Telegram channel......,,,,,,
Love the instructions man great video. Thank you! I've been trying this and keep getting a Grey mold floating on the surface. Any tips?
The reason why this works because many fungi optimal carbon source is malt extract and optimal nitrogen source is peptone / yeast extract
Bless
600ml water
12ml Syrup (18-20g)
LME 1.2g
1g Peptone
magnetic stirbar
30min pressure cooker
*At 15 psi
Is this recipe per 1qt jar ? How may jars can this make ?
Lol nevermind
600ml @@MycoTooMushed
12 ml is 12gr not 18-20gr
Ive seen this channel since it had two videos, I’m attracted to your voice. Keep coming with the vids.
He does have a very professional voice, handsome, dare I say
Pause
Gay lol
@@llaves973 very 🤤
Gayyy
Cool
How about SSLC? Adding like 0.5% of Agar to the mix. Love the vids, mush love
Wow amazing recipes
Wonderful job keep it up
🌮🌭🥘🍕🥗🍱
Thanks for taking the time and making the effort to make thing easier for me! much love from South Africa
Mush love 🍄
Hello Bro. Hope you're doing well. What are your thoughts on just adding peptone to like your regular corn syrup LC. For example -i got premix LC stuff. Agar powder. Peptone. Shit ton of honey ect. Is peptone just a good gram to add to it all? Take care man🍄🙏🤘
My LC is coming out the color of Miller Highlife after PC'ing. Idk how yours comes out so nice and clear.
Instead of mixing all the ingredients with a spoon at the end, drop the mixing bar in first and leave it on the mixing plate, spinning, while you add ingredients to make it a little easier on yourself.
The corn syrup always messes up my stir bar if I do it this way
Yay for metric 🎉
😄
Having problems getting peptone, is there another name for this product?,keep up the great work much appreciated..!!thank you.
I see that we put the foil over the lid to protect the filter, from water right? Does the water in the jar not create steam from the inside while pressure cooking, and wouldn't that get the filter wet either way?
Would this recipe work if inoculated with tissue instead of agar? I keep getting very murky LC after about a day or two and was wondering if the peptone made it more prone to contam with tissue vs agar sample.
Hey may jar came out...and its like GOLD! Like super dehydrated urine color...Is that ok or did I add to much Malt or syrup any help would be appreciated.
Philly, I picked up the olive stuffer from your Amazon links for agar punching - how do you sterilize it?
I love how you mix by hand and then add a stir bar right after 😂
How do you visually identify contams? Mold looks so similar to mushroom mycelium, and I have some growth that looks like mycelium but is... threaded differently idk how to explain it all clumps with the normal looking mycelium too
Has anyone tried preparing grains with peptone? I’d be wary to add it to substrate for contamination risk, but seems like it could help grains colonize faster and potentially promote more vigorous growth?
I use 5g per 5lb dry in my simmer, also found that brown rice is a banger for nutes, so when I make 1lb bags (basically a full quart) I throw in 1 tbs of dry brown rice before I pc, I will never go back
What temperature is best for mycelium growth in your LC?
PGT thanks for all that you do 👍 🙏
I wanted to know if adding peptone bacteriological to your Agar recipe would have a positive effect on my plates?
Of course it will 🔐
I've recently seen a few videos where they REDUCE the nutrition in the agar mix, and claim it increases chance of rhyzomatic mycellium. The idea is reduced nutrition causes the mycellium to have to stretch out to find nutrients. Too nutritional of an agar mix might cause nothing but the fluffy pomentous (sp?) Mycellium to grow in your plate...
If I use this recipe and add agar powder to set it. Will this work as petri agar and slants?
I only use corn syrup when I need to isolate from contams caz it hampers myc growth a LOT. only benefit is that it is clear. my goto is just LME + wyeast . making real potato tea is really really good. Sometimes I will use oak twigs(remove bark/make tea) as a nute boost if the stain needs to be re-invigorated.
Hey what RATIOS do you use for just LME and (nutritional) yeast? One guys saying 10 grams per500ml I think!?! I like to fill to 600ml.. can u help!? Thanks!❤🍄
So u use potato tea instead of LME? I live in South America and can’t really get Lme or peptones.
they all have different outcomes for what u need to do. if ur just starting, u really can use anything that has sugar or starch. ur main concern is cooking to sterile. getting ur stuff clean & sterile is the main concern. after that just experiment and take note of what happens. yeah, LME is a luxury/not needed.@@SachaLotus
potato is hard to get clear if I remember correctly, so if u need to see whats going on i would suggest something more pure like karo. i do remember potato being fast tho. the myc said yum yum, i like potato. the karo, is a little slower(but crystal clear) and can give more time to work like do an isolation or separation.
i use a clear LC to find the winners and make sure everything is clean. once u get a colony that is hardy hungry and ready to rock, use a nute mix that has fast growth for expansion. the LC mixture that is used for expansion might not be clear or have a good color, but it'll get consumed fast so u can achieve a good volume. ofcourse, its vital to get it clean first so u gotta b able to SEE it in the beginning. i suppose u could do all the work on agar, but doing that work in the LC is my goto technique. plus agar is a lot of steps.. i do all that in the LC and have gr8 success. I like the small surface area of jars instead of the wide agar plates
consider > those little guys are hungry and stuck in their home. they will eat whatever caz they gotta survive. I bet even white sugar will work.
p.s remember to leave one jar NOT innoc'd as a control so u know ur technique was good. do all same steps, just no innoc.
-love and light@@SachaLotus
@@tdtrecordsmusic thank you so much for taking the time to reply 🙏, I really appreciate it. I ended up doing two different recipes. For one I used a 2% honey, and on the other one I used 2% honey plus a tiny bit of potato tea plus less than 0.1 gram of a collagen capsule (I read in a comment that it’s basically peptones) . So wish me luck and hopefully they all do well. I’m still gonna do some agar plates to test for contaminations in the near future. Great tip about leaving one LC jar not inoculated to test for contams. Mush love from Peru 🇵🇪🙌🏼🙏
Mr.PGT, can I use water from my well since there’s no chlorine in it? It has minerals in it though. I’m loving your videos! ❤
Yes you can 🙏
Can I use a steam bath(like the popcorn no pressure cooker tek) to sterilize if I don't have a pressure cooker?
What magnetic stirrer do you recommend?