Virology techniques
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- čas přidán 20. 03. 2020
- ssRNA: virology techniques introduces some of the most common indirect laboratory methods used in modern laboratories to study and quantify viruses. These are: virus propagation in cell culture; plaque assay and the quantification of virus infectivity; detection of virus proteins using specific antibodies with the example of immunofluorescence microscopy; and nucleic acid amplification like PCR and RT-PCR.
Written and directed by Irene Petropoulou
Co-directed and edited by Andrew Tucker
Main scientific advisor: Dr. Christopher Clegg, Dr. Eric J. Snijder, Dr. Ernest Gould.
Chief Animator: John Bardakos
Narrated by Marianne Graffam
Fantastic
That's a whole lot of assumptions on the process behind techniques and mechnism behind viral infection made in one video for something of nano-sized, can only be seen under electron microscopy whose images are static, black and white, can only grow inside a host cell and indirect techniques can only be used to infer its existence which sounds rather contrary. How can one know the existence of this nano-sized virus particle if electron microscopy cannot view inside the cell? You're telling me this is all interpretation? Sounds more like speculation.
How do you know these dead cells or plaque formation is resulting from viral infection and not some other confounding variable? For example, the use of anti-biotic and anti-mycotic drugs in cell culture can be poisoning cells especially the use of trypsin to break down the proteins in cells. The cell culture is outside the huamn body which means it will not receive the level of homeostasis to regulate a cells electrolyte, pH, elimination of waste product etc. and thus will die quicker outside the body. Were you taught to use controlled variables? if so, could you please show us a step by step process for the isolation and purification of a virus with the use of controlled variables? and *detail* how the controlled variables were prepared.
can you please upload a video on the basic lab experiments on virology?
Why?
How study virology lab
How name virology
How culture viroos
Procedure lab
Type viros
Pseudoscience