Hello Miss Tatum Sweet, I normally don't leave comments on CZcams videos. However I have watched many videos about Tissue Culture, and I have found your's to be the most useful and practical. I cannot help myself from saying thank you for your videos. The time spent in composing your TC videos was not in vain. You have completely persuaded me to get involved with TC. No other CZcams videos or CZcamsrs have ever done that. They have all made it seems very high and lofty, something the the average DYI type of person would not dare try. However you have succeed in persuading me that I can do it by myself. Thanks again! I cannot fully express the level of gratitude I have for your work. I have three questions. 1)- If not moldy, Can the media be reused with different types of plant? And if yes, for how many times? 2)- What is the difference between the multiplication media and the rooting media? 3)- Is it toxic to dispose of the medias in the environment?
love the videos! i’m just wondering do you ever tc nodes or pieces from existing/mature plants that weren’t plantlets from someone else previously tissue culturing them? if so, i’d love to see that as well!
@@cockatoode705 LOL, I just ordered 16 - 7 volt batteries off of Amazon thinking it was my house too! Dog was hiding in the corner:) Great videos though, and I was out of those batteries so now I have them for next time!
Same. I've watched several videos in a row and kept getting up. The wife got home and I told her she has GOT to find the smoke detector. And let me know where she finds it because it's the best hiding spot ever. 😂
Love your videos! are I appreciate the effort to add a different clip, even the original clip was not recorded. I have a question, do you sterialise your distilled water? If yes, how?
Great video! Just one question. When you’re separating them deciding what to keep in multiplication media vs which ones to plant, what do you look for? Do you try to keep a root on each piece that you are transferring?
I love your videos! Thank you! Have you tried using rubbing alcohol or hydrogen peroxide instead of bleach? I know a lot of medical facilities rely on rubbing alcohol and hydrogen peroxide for disinfecting. I wonder if it would be an acceptable alternative to bleach.
Thanks so much for an in depth video! I have a couple questions... How do you know when the explant is mature enough to divide? How do you know when they're mature enough to pot up instead? Also a separate question...is the initiation phase necessary before moving to multiplication media, or is it fine to jump right to multiplication after you sterilize the explant? Thanks so much! Looking forward to the next video :)
These are great questions! I wish I would have been able to include the portion of the video that got deleted because I showed the plants up close to where you could see the size. I’ll be sure to get those in a future video though! I extract plantlets that are roughly 1/2” in size. Honestly, I typically skip the initiation phase but most of the plants I work with were already in TC to begin with. If you are taking an explant from a mother plant, then it kind of depends. Some plants need initiation and some are fine without it, so it’s kind of just trial and error for every plant! Thank you for following and I’m having so much fun creating this series❤️
Congratulations! You have been selected as one of the winners for the giveaway:) Please reach out to me on one of my socials or email me at tatumsweet94@gmail.com so we can make arrangements to ship your prize! Thank you for your support💙
When you say, "10% bleach", do you mean 10% NaClO solution? or a 1:10 dilution of (fresh) grocery store bleach (which in my country is 3.5% NaClO, I believe a little more in yours)?
Hi! If you buy them online, would you mind sharing the link for your containers? The ones I've been using have PET lids that melt in the microwave, so they have not been very useful. 😓 Thank you for the videos! You've already saved me a couple months of trial and error!
Hello Miss Tatum Sweet, I normally don't leave comments on CZcams videos. However I have watched many videos about Tissue Culture, and I have found your's to be the most useful and practical. I cannot help myself from saying thank you for your videos. The time spent in composing your TC videos was not in vain. You have completely persuaded me to get involved with TC. No other CZcams videos or CZcamsrs have ever done that. They have all made it seems very high and lofty, something the the average DYI type of person would not dare try. However you have succeed in persuading me that I can do it by myself. Thanks again! I cannot fully express the level of gratitude I have for your work. I have three questions.
1)- If not moldy, Can the media be reused with different types of plant? And if yes, for how many times?
2)- What is the difference between the multiplication media and the rooting media?
3)- Is it toxic to dispose of the medias in the environment?
love the videos! i’m just wondering do you ever tc nodes or pieces from existing/mature plants that weren’t plantlets from someone else previously tissue culturing them? if so, i’d love to see that as well!
Your alarm is driving me nuts
I’m so glad I’m not the only one
😂😂😂
OMG... I dont hear well and just spent the last 45 min tearing my house apart looking for smoke detector beeping. CHANGE THE BATTERY .
I changed it!! I’m sorry🤣
I did the same hahaha. I was in bed, got up and looked, decided I'd sleep through it, then woke up and watched this video and facepalmed
@@cockatoode705 LOL, I just ordered 16 - 7 volt batteries off of Amazon thinking it was my house too! Dog was hiding in the corner:) Great videos though, and I was out of those batteries so now I have them for next time!
😂😂😂
Same. I've watched several videos in a row and kept getting up. The wife got home and I told her she has GOT to find the smoke detector. And let me know where she finds it because it's the best hiding spot ever. 😂
Man you are stunning !
Your channel is amazing! Keep doing your awesome job! Thank you very much
Love your videos! are I appreciate the effort to add a different clip, even the original clip was not recorded. I have a question, do you sterialise your distilled water? If yes, how?
Your videos were great! Thank you 😊
Thank you for sharing tisue culture video...
Have any of your caramel marbles shown variegation btw? Love your videos!!
You did great, keep at it! Also, change your batteries please!
Great video! Just one question. When you’re separating them deciding what to keep in multiplication media vs which ones to plant, what do you look for? Do you try to keep a root on each piece that you are transferring?
If they have roots, it certainly helps to try and keep those, but roots aren’t necessary! I just look for plantlets that are about 1/2” or bigger.
I love your videos! Thank you! Have you tried using rubbing alcohol or hydrogen peroxide instead of bleach? I know a lot of medical facilities rely on rubbing alcohol and hydrogen peroxide for disinfecting. I wonder if it would be an acceptable alternative to bleach.
I use alcohol for disinfecting and it's effective, but the fumes are pretty bad. I haven't tried bleach yet.
Thanks so much for an in depth video! I have a couple questions...
How do you know when the explant is mature enough to divide? How do you know when they're mature enough to pot up instead?
Also a separate question...is the initiation phase necessary before moving to multiplication media, or is it fine to jump right to multiplication after you sterilize the explant?
Thanks so much! Looking forward to the next video :)
These are great questions! I wish I would have been able to include the portion of the video that got deleted because I showed the plants up close to where you could see the size. I’ll be sure to get those in a future video though! I extract plantlets that are roughly 1/2” in size.
Honestly, I typically skip the initiation phase but most of the plants I work with were already in TC to begin with. If you are taking an explant from a mother plant, then it kind of depends. Some plants need initiation and some are fine without it, so it’s kind of just trial and error for every plant!
Thank you for following and I’m having so much fun creating this series❤️
Nice still air box
numba one! :-)
Hi I notice you don't use any fungicide when acclimating is this not necessary ? Or rooting hormone ? That is awesome if you can skip that step :-)
Congratulations! You have been selected as one of the winners for the giveaway:) Please reach out to me on one of my socials or email me at tatumsweet94@gmail.com so we can make arrangements to ship your prize! Thank you for your support💙
Also, no, I don’t use any fungicide or rooting hormone:)
When you say, "10% bleach", do you mean 10% NaClO solution? or a 1:10 dilution of (fresh) grocery store bleach (which in my country is 3.5% NaClO, I believe a little more in yours)?
1:10 dilution! I think mine is 7.5%
Hi! If you buy them online, would you mind sharing the link for your containers? The ones I've been using have PET lids that melt in the microwave, so they have not been very useful. 😓 Thank you for the videos! You've already saved me a couple months of trial and error!
I have the link to my containers listed in the description box of my lab tour video! I believe it’s Part 2 of the series:)
@@TatumSweet Ahhh, I see it now. Thank you so much! Sorry for the trouble. 😬👍 Very excited to sterilize my next batch!
@@wolfgangwachtel7787 no trouble at all! Good luck on your TC journey!
How are you i hop you very happy
Can you replace that battery in your smoke detector? You're too fine to go that horribly.
😂😂😂
hi! how you sterilize the plastic container?
Check out my media making video! I think it’s Part 3. I go really in depth with my sterilization process❤️
Hi sweet
Dang!