TISSUE CULTURE 101 | PART 6 | Philodendron & Monstera + Bonus Formulas
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- čas přidán 5. 09. 2024
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Video Mentions
My Amazon Lab: a.co/iymVlzP
MS with Vitamins, Sucrose, and Gelzan: phytotechlab.c...
BA Solution: phytotechlab.c...
NAA Solution: phytotechlab.c...
PP5 Deli Containers: www.webstauran...
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It's like to see a video of taking a mature philodendron and selecting the right spots to cut to start the tc process. These videos are great. I have a lab and I already do most of this with mushroom cultivation but it's interesting seeing plants in agar.
You only do culturing 😍
I love the chitchatty part of these videos
I get ready for my day watching these videos and feel like I’m hanging out
I would love to see you establish a tissue culture from raw plant material. Maybe you could do some shorts for instagram on facebook just following the culture we do together
I am currently working on a sterilization technique for raw plant material. I’ve only had partial success so far. I want to get something down that is more consistent before I share! It’s in the works, though!:)
@@TatumSweet awesome looking forward to that. This has been an informative series so far!
Maybe use a pressure cooker
I like ur chatting. Cute :)
Thank you.. I don't think I've ever commented on commented video before... but thank you so much. For this I've wanted to learn For awhile now, and you are the only one I found that really helps
This is perfect! I'm trying to tissue culture philodendrons as well. One question, have you ever used keiki paste instead of the 6-BA? I know they are both cytokinins but I didn't have any 6-BA on hand so i put a tiny bit of keiki paste in it... also I like your more relaxed approach to the instructional videos, gives me more faith in doing this at home!
The chitchatted part is a little bit nice makes it feel a little more personable
Hi. Loving your videos. I am just wondering if you know how to take cuttings of a whole plant to turn it into tissue cultures. Very interested in this. Thank you would be much appreciated, as i can not get the plantlets here in Australia.
If you show the display text on below for media compositions it's very good for learning stages.. Anyway thanks for deep explanation in each video ..... Thank you🙏
If i mix the hormone stock solutions myself, for example BAP ans NAA/IAA, for how long can they be used when stored in cold temperatures before they expire?
I have looked at your video's and there is sooooo much I do not understand. I need to find something that starts from the beginning. Then I will come back hopefully with a better idea of what you are talking about.
thx for video , is BAP same as BA? cannot find BA in uk somehow
Thank you 😊 💓 your shoeing everything I want to know. And I don't mind the chitchat. I would be talking to myself while doing this. So honestly I enjoy hearing more.
Congratulations! You have been selected as one of the winners for the giveaway:) Please reach out to me on one of my socials or email me at tatumsweet94@gmail.com so we can make arrangements to ship your prize! Thank you for your support💙
Question: how do you figure out that your media sterilized in the microwave? In your previous video you mentioned that it takes you 7 min but that we need to figure our time out with our microwave. How do you understand what is the optimal time?
Is this protocol for multiplying or growing?
Very cool! What's your protocol for Philo+Monstera?
Awesome and useful video! Also love your nails.
Are you able to tissue culture with a Monstera Deliciosa node? I would love to see what your Monstera look like when they are making more shoots. Thanks 🤓
What is the concentration of the growth regulators you are using? You just mention 1.0ml of BA and 0.1 IAA, but not what the concentrations of the solutions are. Thanks!
Each reagents are in description of the video and.the concentrations are given from the manufacturer. However, it would be good to add this information directly in the video :)
Great videos, do you use storage solution for you ph meter? if not it will have a short life, small piece of sponge in cap and a few drops of storage solution. I upgraded my ph meter due to frequent calibration issues with that model, mostly bumping or not delicate handling throwing off the metering. Thanks for the videos, inspiring.
With a lab like that you could grow orchid seeds and make new hybrids. Or grow fungi
Idk if it would be overkill or potentially damage the plantlets but could we include something like a germicidal UV light in the sterilization protocol?
Hi 👋 can you kindly give the formula for the rooting medium aswell please
Hello Tatum! Thanks so much for making these videos, they have helped me so much. I have just made my first batch but I made one mistake. I forgot to top it off with the distilled water to reach 1000ml (1 liter). I did not realize until after I did everything. Do you think I should still leave my plants in that media or make a new batch?
Also, I was wondering if tissue culturing anthuriums is possible. If so, do you have the protocols for that?
Thanks so much.
Hey Emily! I’ve totally been there before. Happens all the time! I would probably go ahead and make a new batch, unfortunately. Without topping it off, it will make the concentrations of the PGRs higher and the pH can also be off. It sucks when that happens, I’m so sorry🥲 also, yes! You can TC anthuriums! I never have, so I don’t have a protocol for it on hand, but I’ll see if I can find one for you:)
@@TatumSweet thanks so much for everything!!
I'd like to know how to package plants for shipping if you do that with your plants.
More media formulas are always welcome.
Oooh that’s an excellent idea! I can do that:)
Hello tatum.... thanks for information.... i want to medium prepare for tissue culture of banana.... so which chemical helpfully nd how to prepare culture at home plss give me information.. nd also give quantities of chemical... plsss
Hi thank you for the recipes.
Would using otc rooting hormone auxins help root faster while acclimating?
I use Clonex rooting gel! It works well :)
They can! I like to add superthrive when I water them and that seems to yield good results:)
Are your 16 oz containers sterilized already or do they just get sterilized by the microwave process too?
What is the wattage on your microwave?
hey , im from Trinidad and i recently started tissue culturing. have you ever tried photoautotrophic methods ? do you have any advice about it ?
I'm a little confusd on the philodendron protocall. So first you put them into 0.5ml of BA with MS and then do you put it into multipalcation media or initiation media?
Then she does her generic media (MS + 1BA + 0.1NAA)
You’re so awesome for sharing all this!!!! I’m so excited to start learning TC :) i would love to hear from you on if you have any experience culturing other genus like anthurium for example! I’m mostly wanting to culture my rare anthurium but so far I haven’t found anyone who uses TC for anthurium
I am also curious about anthuriums, I am presuming its not needed as much due being able to grow from seed generally i larger quantities ☺
Hello from Texas , any experience with this media? (MS Cannabis Multiplication Medium w/ meta-Topolin).
I like the chit chatty stuff, though when I’m editing my own videos I cringe at me speaking 😅😂
Hi Tatum,
I've been binge watching your videos and was wondering if just MS media will help to grow plant tissues without the hormones?
Thanks you so much for uploading the videos and informing us about tissue culture. I've known about TC for a long time now but wasn't exactly sure of how to start. Watching your is really informative and helps make me less afraid of trying to start my own plant tissue culture.
Do you only use plantlets or do you also take tissue from your own plants?
Mostly plantlets but I do have a PPP and a Monstera Deliciosa that I took from my own plants!
When you get a 10pc bag of philodendron from your supplier, and you want to start creating more.. Do you use the "shoots" protocol and place 1 pc per container? Basically I'm missing a link between what to do when you get TC philodendron in and want to make more.
I’ll go more in depth in the sterilization video and the show you the entire process, but yes! I usually pick out about 5 out of the 10 to put back into TC, then I sterilize, then I put them into the “shoots” media. Sometimes I put every single one into their own container and sometimes I put 2-3 in the same one. No rhyme or reason there, just sort of depends on what I have on hand:) hope that helps!
@@TatumSweet awesome. So the single plantlet will grow multiple shoots and then you will septate then and put in multiplaction roots and shoots media?
@@girlwiththemostcake865 exactly!😊
Get yourself a pressure cooker boo!
I really need to!