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Stipe & Cap Mushroom CO
Registrace 2. 12. 2023
A place for the home mushroom cultivator, myco-nerd, citizen mycologist, or fungal enthusiast to view content that serves our wider community.
No fluff, long introductions, no-nonsense to-the-point cultivation content.
Always learning, always growing.
Join me on Discord:
discord.com/invite/shroomery
No fluff, long introductions, no-nonsense to-the-point cultivation content.
Always learning, always growing.
Join me on Discord:
discord.com/invite/shroomery
Open-air inoculation shenanigans. MS spore syringe to agar.
Inoculation of 1.5% nutrient agar @ 2% gelling strength with a multispore solution using a disposable cell spreader.
2 plates germinated while the others remained blank.
When using a SAB or laminar flow, aseptic technique is extremely important, this is an example of that reality.
Still air and laminar flow are advancements in the application of aseptic techniques that add layers of assurance, operating without them is possible but less reliable at scale due to variability in environmental factors that will affect the probability of success in an open environment.
2 plates germinated while the others remained blank.
When using a SAB or laminar flow, aseptic technique is extremely important, this is an example of that reality.
Still air and laminar flow are advancements in the application of aseptic techniques that add layers of assurance, operating without them is possible but less reliable at scale due to variability in environmental factors that will affect the probability of success in an open environment.
zhlédnutí: 380
Video
Manure Coir Vermiculite Brown rice flour (MCVB) substrate | Part 1
zhlédnutí 837Před 6 měsíci
The following is a combination of features adapted from Baba Yaga's manure water Tek, Mateja's coir/BRF recipe, PF Tek, V.L's EF-Tek, and in-vitro bottle Tek. Manure tea will be used to hydrate the coir/verm/brf recipe to avoid the requirement for grain spawn and to take advantage of all ingredient's ability to be steam-sterilized at atmospheric pressure in the same way we would process PF-Tek ...
23q Presto Steam Penetration test
zhlédnutí 945Před 6 měsíci
The lid holes are actually 1/4"* Testing the load penetration capabilities of the 23-quart Presto pressure canner by inserting type 4 steam indicator strips into grain (un-cooked/un-hydrated) jars to ascertain both penetration and cycle function. One cubic foot of water will become 1646 cubic feet of steam when evaporated at zero psi gauge pressure and a temperature of 212°F. One cubic foot of ...
How to operate a still air box (SAB) for home and commercial mushroom cultivation
zhlédnutí 10KPřed 8 měsíci
Still air is the name of the game in a still still-air-box (SAB). A still internal atmosphere allows for microscopic contaminant particles to rest on the floor of the SAB, turbulence generated by movements within the "box" will disrupt the settled particles, potentially resulting in contamination. Never modify a SAB with gloves, blowers, or fans, filtered or otherwise. Do not fill your SAB with...
Pressure Canner trivet upgrade: 23 quart Presto
zhlédnutí 523Před 8 měsíci
2" zinc machine screws, washers, and nuts. pretty straightforward. When loading quart or liter jars for mushroom cultivation, you will need to adjust the leg length to accommodate 10 jars in the 23q Presto. Leg length should be 1 1/4".
How to grow mushrooms: Mycelium plate transfer (A2A)
zhlédnutí 397Před 8 měsíci
Plate transfers can be used to make plates intended to be used as inoculum, or for separtion from contamination (cleaning). One mater plate can produce many others for culture expansion.
How to grow mushrooms: Inoculating liquid culture (LC)
zhlédnutí 313Před 8 měsíci
Always use a proven, pure plate culture to inoculate liquid culture. Spores carry potential contaminant organisms which should be avoided at all costs. Simply place a myceliated wedge of agar into the vessel, place on a shelf at room temperature to colonize.
How to grow mushrooms: Cloning a mushroom
zhlédnutí 367Před 8 měsíci
Always make sure to pull the mushroom fruitbody apart, don't cut it or you'll risk transferring surface contamination to the interior mushroom flesh. This process is the same for all cultivated species. With a sterilized scalpel, remove a small chunk of internal tissue and place it in the center of a petri dish, then wrap the plate and place it on a shelf at room temperature to colonize.
How to validate a steam sterilizer for mushroom cultivation
zhlédnutí 350Před 8 měsíci
Autoclaves require weekly validation in a laboratory setting to ensure proper function and quality control. As home cultivators or business owners, we don't require this level of scrutiny, however, it may be important to verify your sterilizer initially when operating an atmospheric sterilizer for commercial production. When validating larger units, simply place a biological indicator ampoule i...
How to make malt extract agar (MEA) for home mushroom cultivation
zhlédnutí 2,9KPřed 8 měsíci
Agar is quite simple when dealing with non-selective media. Standard 2% gelling and nutrient strength are all that are required to produce healthy fungal colonies. Amending with peptones, yeasts, etc, is not necessary but if that's your thing, to each their own; for most of us this will be all you'll ever need: for a sleeve of 25 @ 2% concentration: 600ml distilled h20 12g agar 12g Dry malt ext...
How to prepare: Wheat, rye, and oats for mushroom grain spawn
zhlédnutí 6KPřed 8 měsíci
Boiling produces the most reliable results when hydrating large cereal grains for spawn production. Boil until the starchy core has turned from white to translucent, then dry long enough for the surface to roll freely, and free of moisture. this process is the same for common large cereal grains used for spawn production: Wheat, oats, and rye berries. load jars with approximately 3 cups, then s...
Agar to grain transfer in a still air box (SAB)
zhlédnutí 661Před 8 měsíci
Find a rack with the appropriate height to match your jars. Transfer from plates elevated to jar height to minimize culture exposure to the open environment by reducing travel time from plate to receiving jar, make sure to loosen jar lids before working. Allow your SAB to sit for at least 60 minutes to allow particles to settle before making transfers.
How to sterilize mushroom spawn bags in a 23q Presto
zhlédnutí 4,2KPřed 8 měsíci
Using 3t Unicorn bags, simply match the gussets and wrap the bag around itself with the filter facing outward. Stack in your Presto making sure to use the appropriate heat source for your pressure cooker. If using an electric hot plate, make sure to purchase a unit with an Infinity switch and the appropriate wattage for the unit. If using gas, ensure that the BTU of your burner matches the manu...
How to inoculate and seal a 5lb sawdust block/spawn bag for home mushroom cultivation.
zhlédnutí 353Před 9 měsíci
How to inoculate and seal a 5lb sawdust block/spawn bag for home mushroom cultivation.
That time I verified the Instant Pot for use as an "autoclave".
zhlédnutí 1,1KPřed 9 měsíci
That time I verified the Instant Pot for use as an "autoclave".
How to build a humidification system for a mushroom grow tent.
zhlédnutí 1,5KPřed 9 měsíci
How to build a humidification system for a mushroom grow tent.
Cultivated outdoor wild Poplar mushrooms
zhlédnutí 198Před 9 měsíci
Cultivated outdoor wild Poplar mushrooms
How to grow mushrooms at home: Shoebox Tek
zhlédnutí 36KPřed 9 měsíci
How to grow mushrooms at home: Shoebox Tek
How to wrap Petri dishes with Parafilm
zhlédnutí 297Před 9 měsíci
How to wrap Petri dishes with Parafilm
How to make filtered jar lids for mushroom grain spawn
zhlédnutí 1,9KPřed 9 měsíci
How to make filtered jar lids for mushroom grain spawn
How to use a spore swab for home mushroom cultivation.
zhlédnutí 695Před 9 měsíci
How to use a spore swab for home mushroom cultivation.
How to pour and store blank Petri dishes for home mushroom cultivation.
zhlédnutí 656Před 9 měsíci
How to pour and store blank Petri dishes for home mushroom cultivation.
How to fold and load spawn bags for home mushroom cultivation.
zhlédnutí 870Před 9 měsíci
How to fold and load spawn bags for home mushroom cultivation.
How to fold and load mushroom sawdust production blocks.
zhlédnutí 224Před 9 měsíci
How to fold and load mushroom sawdust production blocks.
Growing mushrooms from spore print | How to use a cell spreader to inoculate agar.
zhlédnutí 279Před 9 měsíci
Growing mushrooms from spore print | How to use a cell spreader to inoculate agar.
A quick flash prep for millet grain spawn.
zhlédnutí 3KPřed 9 měsíci
A quick flash prep for millet grain spawn.
How to streak a dry spore print to agar for home mushroom cultivation.
zhlédnutí 396Před 9 měsíci
How to streak a dry spore print to agar for home mushroom cultivation.
How to make easy Instant Pot liquid culture for mushroom cultivation.
zhlédnutí 3KPřed 9 měsíci
How to make easy Instant Pot liquid culture for mushroom cultivation.
Would you please test 2 more jars with 0.5 inch of water; one with tightened [2 peice ring] lid; one with a "finger tight" lid? I'm being told on Reddit that you have to vent the trapped air **in the jar** to achieve sterilization. I believe that the venting is to allow a vacuum to be formed after cool-down. Some mycologists find undesirable because it sucks in "dirty air" (we'll ignore the flow hood) when opened. I believe that a tightened lid will still sterilze, but result in no/little vacuum after cool-down. The lids and jars should be okay (unlike in your dry test) because the pressure inside the jars will increase because the water will turn to steam.
just a heads up doggie bags make perfect liners for shoeboxes and they come in a nice roll
Great tip 👌
Marilie Roads
McCullough Drive
Where did you buy that one?
I designed it and had it fabricated.
Whats your experience with corn...vs oats?
Corn is very clean but is too large with too few inoculation points for my taste. Oats can be troublesome, it depends on the source. I don't recommend either.
Huh? I have not had a problem with oats. Trying to see of going to corn was worth it. Doesn't sound like it though. I rarely have contamination issues. Can I ask what your grain preference is? Seems like my cultures actually do better when I switch things up once in a while. Not wbs or rye thought. Rye is kind of a pain to get right and is ridiculously expensive here. Everyone keeps talking about this drippy tech which seems pretty dumb but you kinda confirmed my suspicions. Not worth it really.
@timdesoto3138 99% of people lack a fundamental understanding of fungal metabolism and life cycle, the "dippy tek" is ridiculous. My preference is wheat or millet.
@@StipeCap wheat is cheap and readily available I just started spawning with it things are awesome so far😁👍
Why are you only worried about contamination during spawn and not the steps after that
Because coir/vermiculite that has been expanded via hot water has no contaminant organisms. Competitor organisms will piggyback on contaminated spawn, infecting the substrate. This is why clean spawn is so important. This is not the case however with manure substrates that can mold when improperly processed.
This AI voice is unappealing. How about a female AI voice?
Did you just assume my gender?
Can milo be done the same? I'm having a hard time dialing in milo/sorghum
Really just simmer until the expand...same shit after that. I haven't really need to boil it. They bust too much when you do. Not as dirty as wild bird seed either.
Dickinson Squares
I know all this stuff but I enjoy hearing and seeing how other people do things. Good video 👍🏼
3 minutes to teach us how to use a pressure cooker? holy fuck. you talk too much and your voice is annoying.
I tried repeating what you did. Right now my substrate is 90% colonized and there is no colonization on my casing layer. Visually the layers are very distinct. I'm concerned that I may have packed my casing layer too compact. Do you think that's a possibility and has that happened to you?
Nope, it's fine. I know folks that use their entire body weight to compress. Be patient.
How much water do you fill into your pressure cooker pot when you do this? I happen to have just bought that same pressure cooker and I'm not sure how much water to put in it. thank you.
3 quarts
Essentially you need enough to not run out while not submersing the jars. Steam under pressure reaches higher temperatures than the boiling point of water which is where the sterilization comes from.
@@StipeCap Thank you I think thats a little less than I put in I used 5 quarts. 3 is safe? thx
@Mushlove1213 3 quarts is what the manual says to use. 3 is plenty.
I am looking to my own first grow and I was wanting to use brf as for my “grain” jars. Would that still work could with this substrate formula. I would still use 1qt of colonized grain
Very informative, thank you. I've watched a lot of videos on mycology on youtube recently, and yours is the only one to cover the reasons not to use to a glove box. Lots of people promote SABs, but don't mention glove boxes. Could be very easy for a noob like myself to see a glove box, and think it's a better/upgraded version of an SAB.
It's always best to know why something works, not just how.
How did this turn out?
New subscriber, I love how information dense and quick your videos are. Zero waffle
Does the insta pot provide the necessary sanitation to do all the things that a pressure cooker does
I’ve had great success laying a layer of vermiculite...cakes in next layer.... pseudo case w verm. Gold n white lasagne 👏
Great videos man, clear and concise. My only suggestion for a video like this though is that it seems a bit incomplete. How are you sterilizing the scalpel? Do you sterilize between each transfer? Are you wearing gloves? I'd love to see your full process
Few people engaged in this hobby are running IR units, they're a bit different than flame sterilization; but yes, flame sterilize until red hot, and cool in the receiving dish. The intention of this video was to show how and where to place dishes in relation to receiving jars, it was not intended to be comprehensive.
I don’t understand how you can get away without drying them. Lol.
Bird seeds don't require drying
thank you when is next vid
How do you prevent the grain bags from blowing up under pressure?
Check out my other video re spawn bags in a Presto.
Purging the pressure cooker? I’ve never done it and will never do it and have always had stellar results.. people need to drop all these old-school methods. Growing mushrooms is quite easy. It’s just very tedious no need to add a bunch of extra mumbo-jumbo to it. Not saying to cut corners, but some corners shouldn’t be there.
It's not "old school", this is how autoclaves function. Dalton's law covers this phenomenon: Quote: According to Dalton's law of partial pressures, the total pressure by a mixture of gases is equal to the sum of the partial pressures of each of the constituent gases. The partial pressure is defined as the pressure each gas would exert if it alone occupied the volume of the mixture at the same temperature. If you don't vent/purge the pressure cooker or autoclave you won't reach the appropriate internal temperature.
im eating portobello because is my favorite and are vary exspensive
Great explanation mate, just wondered whats the shelf life of the jars you dont use straight away? Cheers
Why millet? What are the advantages over other grains, or brown rice?
It’s a personal preference I’d recommend using brown rice since it’s a lot cleaner than feed grain
Also feed grain is a lot cheaper than brown rice since it’s for human consumption
Hi, thanks for this valuable info! And just starting and I'm going to remove the gloves from the holes of my SAB (it's just an inverted tub). I'll Also add something to put my materials a couple cm above the floor of the SAB. I wanted to ask you, if I Get more serious in the future, do you think making a proper SAB like this would minimize contamination reliably (providing good sterile technique) or is a laminar flow clearly better? Thanks!
Both have advantages and disadvantages and can have different applications. Working with contaminated wild cultures or spore printing is best done in a still-air environment. Still air is often employed in areas that don't have access to a cheap/reliable power source in developing countries. Laminar flow allows for freedom of movement, workflow, and a greater volume of work when the appropriate infrastructure is available. Having both available is the best-case scenario. Sterile technique is key no matter what you choose.
@StipeCap thanks for this detailed answer. I'm just a beginner but I'm Also highly motivated. I'll see how I can manage to make myself a proper and big enough SAB for now. I'm also trying my Best with sterile technique, exposing grains and spores as little as possible to the air and not putting my hands above this materials. It's more challenging that it seems, isn't it? If I can, I would like to know your opinion about this: should I, provided I Get a big enough SAB, prepare the cakes inside of it or not? Because it seems overkill, but, at the same time, we're really careful while preparing the grains, but not as much with the bulk, and I happened to Get contams at the bulk colonizing stage.
@martindamasseno5164 Still air and laminar flow are for the sterile culturing phase of cultivation. Producing pure culture on agar, the inoculation of media like spawn, liquid culture, grain to grain transfer, etc. Anything past that phase that doesn't involve nutrient-dense substrate or media can be handled in the open air.
@@StipeCap thanks, man, really. Much appreciated!
Hey just wanted to say thanks for the knowledge
@StipeCapI so appreciate the contribution you make to the cultivation community, here and on the Shroomery. There is so much misinformation out there and people are still fanning their tubs and adding unnecessary stuff to their substrate. Your channel is a welcome relief from all of that nonsense. Thank you for your videos.
Are you growing indoors? Does it stink? Would you recommend growing in a bedroom?
Yes, indoors. No, it doesn't stink, any room is fine.
Thank you for this!
So you boil for 30 seconds? Or simmer?
Boil the water, remove from heat, soak seed in water for 30 mins, drain, load into jars.
How do you get the spawn? (Also whats a flush) I wanna try making mushrooms for myself, any tips would be great. Another question, whats the difference between the too layer and substrate? And whats the grain?
Start with a grow kit, read a ton of “shroomery” it’s a forum about growing in internet, start with little, and never do something without doublecheck in mushroom cultivation, I learned that the hard way
Don’t start with grow kit do a bunch of reasearch about how to make your own grain jars there is ways to cultivate mushrooms for less than 50$
Agree on simplicity of SAB and usage. On the part of adding fan and filter internally and gloves access, thats depends on use and environmental factors. Not to say it is good, just an added layer of getting better air quality control within the box. The inter filter and fan doesn’t need to be turn on during actual work, just need to pre-clean and turn off beforehand.
Sorry I meant jars , not nepals
Thanks for the video, are you putting the millet in to the Nepal’s while still wet? Thanks
Hey man, I appreciate all of your information and work. You do a really good job at explaining everything, thank you for that. I have a few questions that I hope you can answer. First, what is the dry weight of millet per bag before you hydrate them? Second, how many of your 5lbs sub bags are you able to inoculate with one of your grain bags? Third, would 14A .5 micron unicorn grow bags would work for this? Finally, I am using a gas stove. My PC is some brand that Canadian Tire carried a few years ago and is no longer available, i've had no issues with jars but do have some concerns that the hot metal could melt my bags. Would bringing the boil/heat up slower be the play here? This last one is a little vague, I may just have to mess around with it a few times but don't really want to dick around with a pressure cooker and stuff melting inside. Thanks again!
I wouldn't recommend . 5 for spawn but the bags should be fine if you keep the flame low.
@@StipeCap Thanks for the reply man, are you able to chime in on the other questions?
@Max-bh2rc feel free to join me at shroomery.org or our discord server for more in-depth information.
@@StipeCap Got it, thanks man. I see the benefit of having this discussion in the community. Ill make a thread shortly.
@@StipeCap Hey, I made a post on shroomery but not exactly regarding the questions which I initially asked you. It is in the gourmet section. If you have the time to respond, I would grateful for your input.
Did you mix your grain with the coir or just leave the grain layer at the bottom & top it off with coir?
Mixed into bottom layer, then topped.
Another question. I just got my malt, but must have misread it as it is liquid malt extract. I am just going to experiment making plates, but do you have a recommendation on how much to use if I was doing the same as your video here?
I've not used liquid so just play with the ratios to see what works best.
Thanks for a clear and concise video.
Hey mate. If I used this method, can I use my instapot pressure cooker like for grain, and just do it longer? or is 60 min also enough in this situation as well?
45 minutes is plenty for liquids.
Dude your videos are amazing....
My first try I cleaned and went in to work like 3 minutes later. Probably a big no no contam city
No BS great video
Much obliged.
So the substrate and casing layer is the same material?
Yep, that's why it's called a psuedo-casing.
I wish you did all weights instead of mixing weight and volume
That's how it's done.
"Shake the fk out of it" yeah way to emulsify your mix lol. Stir bar works wonders if your lucky enough to have one with a stir plate.
No need to shake at all, the PC will take care of everything.
followed this tutorial to the T and first one is blobbing. Will see how the Other 8 do soon. pe👍
Pe has a tendency to blob as I understand. Good luck.
@@TeaTellyWMaria yes but this tutorial is supposed to solve that. it did for me. 50/50 of the time.
@ixxgxx keep dialing it in and you'll get it 👍