How to operate a still air box (SAB) for home and commercial mushroom cultivation
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- čas přidán 9. 09. 2024
- Still air is the name of the game in a still still-air-box (SAB). A still internal atmosphere allows for microscopic contaminant particles to rest on the floor of the SAB, turbulence generated by movements within the "box" will disrupt the settled particles, potentially resulting in contamination.
Never modify a SAB with gloves, blowers, or fans, filtered or otherwise. Do not fill your SAB with flammable vapors or operate a torch within the SAB, certainly never combine flammable alcohol vapor with open torch flames.
Your explanation is perfect. I came to the same conclusion of what you're explaining. Your video confirms my theory. Thanks a lot!
Omg I came here to say that exact same thing
Hey next time use a wet towel at the bottom of your SAB and spray heavily and the wet towel traps the particles and they aren’t airborne anymore has work wonders for me
That works fine for a small tote, not necessary for this large SAB.
Very informative, thank you. I've watched a lot of videos on mycology on youtube recently, and yours is the only one to cover the reasons not to use to a glove box. Lots of people promote SABs, but don't mention glove boxes. Could be very easy for a noob like myself to see a glove box, and think it's a better/upgraded version of an SAB.
It's always best to know why something works, not just how.
First SAB I've ever seen that's worth having.🎉
Totes are the perfect introduction-level quick fix, but anyone serious about long-term use should consider something like this, it doesn't have to be entirely Plexiglas, but something similar in size, at least.
Would you consider making a video about how you did this marvel of a SAB?
I had it built by a plastics company.
Clicked the video because this SAB concept caught my attention
Have you not heard of a still-air-box?
@@StipeCap I’m fully aware of the SAB, but I liked this design
Oh, I see. Thanks for the clarification.
Very informative video, and I'm jealous of that SAB.
Thanks for a clear and concise video.
Actually very good and scientifically sound information. You, Sir, are helping out.
excellent explanation, very important video for home cultivation.
Much appreciated. There seems to be a great deal of confusion concerning the application of both still air and laminar flow. Both produce excellent results on their own, but should never be combined.
Agree on simplicity of SAB and usage. On the part of adding fan and filter internally and gloves access, thats depends on use and environmental factors. Not to say it is good, just an added layer of getting better air quality control within the box. The inter filter and fan doesn’t need to be turn on during actual work, just need to pre-clean and turn off beforehand.
Thanks for sharing and deftly, you gotta a killer SAB
Much abliged.
Great video
Your generosity of information is honorable. You've earned a sub and likes.
Everyone trying to make a dollar from Patreon, I have no problem giving it away for free.
Willy Mycvo, PGT, and other "big names" of CZcams cultivation are all a bunch noobs attempting to make a living from other people's intellectual property.
@@StipeCap That's quite the accusation. Care to give examples of how each of them are stealing IP? What IP are they stealing, from whom? It seems surprising that such prolific CZcamsrs are supposedly stealing IP and not getting sued for it.
We're talking about techniques authored by semi-anonymous individuals to cultivate what was once illegal mushroom species.
There are no legal actions that can be taken against individuals who have misrepresented information they've found published openly on the internet.
This is a widely known fact in the online mushroom community, it's folks who are new to this community that don't recognize this reality.
Unless of course, you believe that all of these folks learned to cultivate in a vacuum and have become so adept independently of others that their cultivation content is pure insight, hard earned experience, and skill. None of them are skilled, none of them have developed their own techniques, and none of them give credit where it's due while they charge unwarranted fees for unsuspecting newcomers to access info they have neither developed nor know how to employ correctly themselves.
They're charlatans.
I see a lot of SAB with the holes centered in the vertical middle, but isn't it better to make them closer to the bottom? So if a stream of contaminated air enters, it does not come over the material
If a draft came through the holes, the floor is where the particles settle, so no, the floor must not be disturbed.
Gracias amigo hongo… amigongo
I do wipe down the "floor" of my SAB before I do work. I like to think I'm removing dust and debris that has settled from previous use.
Clean your SAB, but there's no requirement for excessive sanitization. If there's dust in your SAB, remove it before use.
You can run the SAB dry, or spray the floor with soapy water to trap particles.
@@StipeCap Can I spray soapy water all over inside the box as soon as I set up it? This way the soapy water captures floating particles and there's no need to wait 3 hours for them to naturally fall down to the floor?
My first try I cleaned and went in to work like 3 minutes later. Probably a big no no contam city
No BS great video
Much obliged.
Loved the SAB explanation! Very helpful! Quick question though...what are the taller racks/trays called, or better yet where did you buy them? Thanks man!!!!
They're just dish racks. Check out my other SAB video, the beginning shows me sourcing the rack.
Great info. I’ve been looking for that taller rack you have there. Is that stainless steel and where did you buy it ? Thanks in advance.
I'm in Canada, so I purchased it from Canadian tire.
How did you get such clean cuts on acrylic? Awesome build man very good craftsmanship
The unit was built by an acrylic fabrication company. Professionally built.
Great, very informative
Does it help if build the bottom of SAB lower and use taller racks, just increasing distance between working levels and bottom of SAB?
Yes, within reason.
Hi, thanks for this valuable info! And just starting and I'm going to remove the gloves from the holes of my SAB (it's just an inverted tub). I'll Also add something to put my materials a couple cm above the floor of the SAB. I wanted to ask you, if I Get more serious in the future, do you think making a proper SAB like this would minimize contamination reliably (providing good sterile technique) or is a laminar flow clearly better? Thanks!
Both have advantages and disadvantages and can have different applications. Working with contaminated wild cultures or spore printing is best done in a still-air environment. Still air is often employed in areas that don't have access to a cheap/reliable power source in developing countries.
Laminar flow allows for freedom of movement, workflow, and a greater volume of work when the appropriate infrastructure is available.
Having both available is the best-case scenario. Sterile technique is key no matter what you choose.
@StipeCap thanks for this detailed answer. I'm just a beginner but I'm Also highly motivated. I'll see how I can manage to make myself a proper and big enough SAB for now. I'm also trying my Best with sterile technique, exposing grains and spores as little as possible to the air and not putting my hands above this materials. It's more challenging that it seems, isn't it?
If I can, I would like to know your opinion about this: should I, provided I Get a big enough SAB, prepare the cakes inside of it or not? Because it seems overkill, but, at the same time, we're really careful while preparing the grains, but not as much with the bulk, and I happened to Get contams at the bulk colonizing stage.
@martindamasseno5164 Still air and laminar flow are for the sterile culturing phase of cultivation. Producing pure culture on agar, the inoculation of media like spawn, liquid culture, grain to grain transfer, etc.
Anything past that phase that doesn't involve nutrient-dense substrate or media can be handled in the open air.
@@StipeCap thanks, man, really. Much appreciated!
What are the dimensions and what’s the black material on the frame of the covers? Great design I’d like to recreate it
Skim through the comments, the dimensions are listed in one of them.
Where did you get that? Or did you build it? I love it.
I had it fabricated, my design. The top, front, back, and bottom are a single piece, 1/4" plexi, with polycarb hole covers, brass screws and grommets.
Covers have foam gaskets. I'll have to check but I believe it's almost 400 internal quarts, worthy of commercial use.
@@StipeCap would be interested in replicating it. It is amazing.
It was a prototype intended for production, but it's not cost effective, too expensive.
@@StipeCap that's a damn shame. Its awesome
One of a kind, but it works great. I can run a load of bags without laminar flow.
SE Asian farms have more still air boxes than US farms have laminar flow. Folks don't appreciate the efficiency and reliability of still air.
Hopefully, it will inspire folks to venture outside the upside-down tote mentality.
Drilling plexiglass is extremly difficult
One of the many reasons I had it made by professionals.
Where did you buy that one?
I designed it and had it fabricated.
Spraying ozium would also help
Soapy water sprayed on the floor of the box can help trap particles.
Did you build that box or buy it?
I had it fabricated.
I guess you had to; I see nothing like this available online. Very well done explanation video. Thank you. @StipeCap
@SamWereb Thanks, and yes it's very custom.
Dude, I would never use one of those. It may work good in a lab environment with HEPA filtered air coming into the room and zero carpeting, but for household use there's too much contamination floating around from carpets especially. I can walk a few steps across a carpet and in the right light I see massive dust particles in the air. Not to mention contamination from kids and pets and the wife's vacuum cleaner. LOL
Where was that SAB built it's. QUALITY ????
I had it built by a fabricating company.
@@StipeCapcould you please share the concept dimensions and the material used to frame the covers? That black material that seems to adhere to the acrylic
@joaorebochooaw6321 The black stuff is crushed foam gasket to prevent leaks. The hole covers are polycarbonate with sunk threaded brass grommets.
Thats a bitchin sab , ist it commercially available ?
Too expensive to sell, unfortunately.
Do you do your flame sterilizing of blades and syringes outside each time and then come back in slowly?
Yes, always sterilize utensils outside of the SAB. The red-hot metal will maintain sterility while you move from exterior to interior.
You can use the same theory as a paint booth, dust particle floating around can mess up a paint job, so they wet down the inside to keep particles from blowing around and was vaccum vented! you don't need to vaccum vent, but use a spray bottle of 70% iso.alchoh. to moisten the interior, but not to the point where you have dripping from the top and onto your items you are working on!
Some folks spray the floor with soapy water to trap and neutralize particles, isopropyl tends to evaporate and produce fumes. Neither is necessary, but I'd choose soapy water over alcohol.
The problem with a vacuum system is it pulls outside particles into the box, and disrupts the still air environment.
This AI voice is unappealing. How about a female AI voice?
Did you just assume my gender?