At Home Micropropagation: In Vitro Plants - 2018 Four Seasons Gardening Webinar
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- čas přidán 28. 08. 2024
- Micropropagation is a form of growing plants using tissue culture in sealed containers. This method allows for new plants to be grown using cuttings down to a number of cells. Learn from Extension Horticulture Educator, Bruce Black, about micropropagation and how you can do this at home using common household items.
If you would like the handouts or protocol discussed in this video, please visit: go.illinois.ed... and email Bruce at brucejb@illinois.edu with any questions you may have. Thank you!
Thanks a lot for sharing this video.
Thanks Bruce for your presentation and all the kind explanation of the process.
Regards from Chile!
Thank you 👍🏼 very much. Regards from Kazakhstan 🇰🇿
You are welcome!
Thanks you for the. I lik the hendouts
And my 2 can share with my
😊
Great video thank you Bruce !
Excellent video! So very helpful, thank you!
Glad it was helpful!
Thank you so much for this ! Really great information !
Glad it was helpful!
Great tutorial, thank you for the systematic explanation.
Really cool! I do at home in vitro orchid sowing, and have put up a series on the subject here on my YT channel. There are many similarities with the process you show here!! It is spoken in Portuguese though. So I guess Orchid Tissue Culture is my next step, and this video is a great starting point! thank you so much!
Details plz for home based tissue culture
Very Cool...Sending email for those handouts.
Thanks for this video. Really great information. I am going to try tissue culture at home soon. I'm going to try using an oven to sterilize like I do when I'm making jam. Can easily reach 120 °C there. Thanks so much for sharing this. Greetings from Iceland.
wow bro you really helped me alot with info and understanding to do this at home really appreciate it
Glad it was helpful!
extremely helpfull as most of the professional chemicals or additives are not eastly available and are expensive aswell so nice to know I could use household stuff to get growing
@@IllinoisExtensionHorticulture
Wonderful presentation. Re: the water bath vs. pressure cooker -- I don't know if this has been answered. Water bath never gets above boiling and there is no pressure. The reason I know this is because I can foods to put up for winter using both methods for different acidity foods. You will not get the same result as a pressure canner using a water bath canner if the higher temperature is needed. Hope that helps.
Thank you
wow Bruce fantastic information !!!
Robert - glad you liked it!
Do you have your grow lights on the sides of the jars as the metal top of the baby food jars block out light? Also when would you use the fan for airflow as the plants are inside the jars. How would the fan benefit the process. Thanks a lot
happy to subscribe here
thank you for this information , i will try it at home , i have one problem ,the inositol , because is not here in my country , but i try look by the pharmacy store if they sell them .
dompig bryan - Good luck!
It was also known as vitamine B8.
this is great! would love to have a read at the documents you showed, where can I find them? great work congrats!
My first batch was 0%, second batch 2 growth out of 16 bottles 😅
Which plant u tissue cultured ?
I was able to find the first resource. Are you able to post the link to the second handout? HANDOUT -Micropropogation protocol?
The second handout can be obtained by emailing Bruce at brucejb@illinois.edu. There are usually specific questions that follow and Bruce likes to be available to help answer those questions if he can.
Do you have a video regarding 2 ways of Genetic Modification , or transferring DNA of one plant to another plant cells. Thanks
Hello, we do not currently have one on genetic modification that I am aware of. This could be a good topic for the future. I will pass the suggestion on. Thank you!
Yes could be very interesting, like for the marijuana how to put more terpenes ( strong odors, colors etc)
Been done in the past months plant smell less (while it was illegal) now that's almost legal all round the world, would be great to recreate the real "skunk piss" smell
excellent..
For doing the TC of figs should i us the leaves, bud stems or apical buds?
At some point in the video you say orchid tissue culture is more labor intensive than, say, african Violets... Why is that so? what kind of extra care do orchids demand?
excellent video and instruction. Appreciate the speaker taking the time to do the step by step for tissue culture at home.
Do you have any recommendations on storing the rooted tissue culture as a means to preserve as backup? Can it be done in a refrigerator?
Yes, absolutely. Using a refrigerator is a great option. When I have stored samples at our office. If they are unused media, I put them in the vegetable drawers. They are white plastic and exclude light. For rooted samples, you can keep them in the refrigerator. Make sure the plant is not sensitive to cold temperatures. Since it is a cold environment, the metabolism of the plant will also be slowed.
Are we worried about the multi-vitamins being heat stable as we boil them?
Thank you for this webinar. I learned a lot from this. Do you have a link for the hand out of the procedures? Can I request to pls share it with me? Thank you
Me to plz
If there is a chance can you plz forward it to me on
Ayman.m.abdelnaby@gmail.com
Hello. Bruce is happy to share the protocol and handout for this presentation. Please email him at brucejb@illinois.edu .
@@IllinoisExtensionHorticulture ❤
Thank you for the excellent presentation, I have a question. can we use cinnamon to avoid fungi?
No because cinimon has contaminence in the spice itself
I have seen people using stems. I am curious if that is technically even "tissue culture" or just "growing from cutting"?
There is a significant difference tissue culture can made a 100 plants where u could made 1 to 10 plants via regular propagation
Great webinar it's been very informative
Can a tissue from a date produce a palm
If there is a chance could u plz forward me the documentation
I forgot to ask about the hormones.
You never mentioned them in the medium preparation
Hello. Bruce is happy to share the protocol and handout for this presentation. Please email him at brucejb@illinois.edu .
Bruce will need to do some research into your question about dates and palms.
As for your question on hormones here is Bruce's response, in a lab setting, we would make a specific auxin and cytokinin solutions for the different medium. Most of the auxin and cytokinin substances I know of are sold for labs. In the at-home recipe, the inositol, fertilizer and vitamin tablet, provide enough of the needed materials to get the plant to begin to grow and divide. The inositol is used in cell wall biosynthesis as well as the transport of plant hormones. There should be enough plant hormones or the ability to make more hormones in the plant piece we are using. By giving it the proper nutrient rich environment, we are providing it with what it needs to regenerate what is missing.
what kind of vitamin like a daily vitamin? great video thanks
Yes, a daily multivitamin. I purchased a daily multivitamin from the local pharmacy for the experiment. Any brand will do.
@@IllinoisExtensionHorticulture Do I need to worry about PH due to Vitamin C and Fertilizer?
@@essoborja763 Great question. The pH of the vitamin and the fertilizer will make the media acidic enough for the nutrients to be available for the explants to take up.
Is there any where to download the pdf of the Micropropigation Protocol for hands on activity??
Never mind I found it.
Would you please tell me where to find it.
@@xaphansinferno163 also curious where you found it please
Do you have this PPT? I will love to get this PPT. Thank you for sharing.
I'm sure Bruce would be happy to share his resources. You may email Bruce at brucejb@illinois.edu
17:20 - Is it possible I can take a fresh plant cutting then blend it in a sterilized blender under vacuum to prevent oxidation, the fresh plant cutting is now a paste-mash like substance, can I quickly place this in the tissue culture tubes with MS medium and expect it to root and grow? Is this even logical and practical? Aldo is it possible to use PDA instead of Agar?
There are lots ofc different gelling agents agar is the most sterile from factory but if the gelling agent is sterile it should work
@@Adamap9 I found out that lab scientists found a better and cheaper alternative to agar, which is “psyllium husk”. They even documented it’s properties to agar and concluded that “psyllium” is better.
@@ShopperPlug thanks I'll update agar to psyllium husk
...there should be a counter in the corner of the screen counting how many "UMM" he said.... :p
-nevertheless good video!
Use time stamps.
Umm