Clonogenic Cell Survival Assay (Colony Formation Assay)

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The Colony Formation Assay (CFA) or Clonogenic Assay is often used to determine which effects a specific drug or irradiation has on cells. This method is frequently used in cancer biology to investigate whether a treatment might be beneficial for tumor cells that harbour a distinct mutation.
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Music: NoMBe - Interplanetary Alignment (CZcams Audio Library) • Video
Thanks for reviewing: Roman D. / romanmdoll
Time Codes: 0:00 Why to use the Clonogenic Assay 0:54 How to perform the Clonogenic Assay 4:22 Product Placement 4:59 Outro
#CFA #clonogenic #assay

[@hanieazari4251]

really appreciate, great explantion

[@Oculus_idealistae]

Thank you!

[@boodymahmoud9100]

How can i calculate the last graph (Survival fraction and concentration) ?

[@TheAlialhammer]

well explained 😀

[@JH-mz4uz]

great!!

[@pausang2196]

What about plating efficiency? PE

[@quangvinhnguyen114]

I think the exact number is 5 ul in 5,995 ml medium right? (in 1:47)
Thank you for the video by the way, it is very helpful for starters in scientific research like me

[@christiangabrielfigueroaes5755]

Would cells attach if they grow in suspension? I'm concerned most of my cells will die given the length of incubation time.

[@henrikslab]

It depends on the cell line, check your cell line and the keyword clonogenic assay in the internet.. for many cell lines you would need to exchange medium in between more often!