How to prepare a Serial Dilution

[@charlottewright929]

Thank you so much i have been struggling recently in the labs and needed more explaination. This really helped

[@sethdjanboakye]

Simple and Short

[@luckyduke7159]

Thank you so much, it was so easy to understand

[@reamageorge9648]

thanks. Illustration very good

[@Abialmedicos]

Jazak Allah😊

[@marcoromo9252]

Good simple explanation. Thanks!

[@user-qf5vu2ji8f]

thanks for the simple explanation 😄

[@osmirod1]

Wow this was great. Thank you.

[@cramberry1175]

Thank you

[@TriniCanCook]

I have to make 100 ml of a 10 fold dilution of vinegar. How do I do that?

[@osmirod1]

Question, how would you apply this to decreasing the number of cells for cell culture just like you mentioned here?

[@henrikslab]

I would measure the cell count of your culture flask and then determine how much cells you want to have per volume...
You can use a 10-fold serial dilution here, might help!

[@Abialmedicos]

It was quite east sir😢..thqnuh so much 😊

[@Palayedeuhm]

Thank you!

[@bahiachibi895]

Helpful

[@sorlos]

Helpful!

[@oldlady8988]

I still don't quite understand. In my case I have colloidal silver 500 ppm and I need to make it into 50 ppm??? How???

[@henrikslab]

To me that seems to be a classical 1:10 dilution

[@randall777]

Were you able to get your silver ppm down after all?

[@prince_vegeta124]

Thank you very much it was an great information

[@halafr5861]

Thanks 😊

[@jeniferhernandez3465]

Thank you 😊

[@rihamalwan9229]

Good explain

[@cristina7882]

Hello, I have a doubt. I'm trying to make a dilution 10^-1, 10^-2, 10^-3, 10^-4 and sample not diluted.
The problem is that I only have 2 ml of the sample and two tubes with 10 ml (each one) with sterilized water.
I'm trying to do this serial dilution, but with only two tubes it's impossible to do it and let the same ml in each tube.
What I've thought is in put 1ml of the sample in 9 ml of the solution in the first tube (in which I have 10ml and take off 1ml previously), so I can get now 10^-1.
With the second tube (I've also taken off 1ml) with 9 ml of solution I obtain 10^-2. But I need to culture two petris plate from each dilution and the sample no diluted, so I take 1ml from each one, 10^-1 and 10^-2.
Now the problem are the followers dilutions, 10^-3 and 10^-4.
If I take 1ml of the tube 10^-1 and put it into the tube 10^-2, I can obtain 10^-3, but because I've taken from that first tube (10^-1) 1ml for petris plates and the other one for 10^-2, I have in that tube 8ml, and I will be taken 1ml from 8 ml (tube - 1) to tube - 2.
I think that wouldn't be right... I'm really lost with the lackness of material I need to solve this. Greetings.

[@cristina7882]

I forgot to say that I have to do it for duplicated, - 1,-2,-3,-4 and not diluted. 5 petri plates × 2 = 10 petri plates.

[@padhmapadhma9273]

Thank sir

[@abitofambience]

Hey sir, the final volume of the ten fold expirement is 9 mL?

[@Quolon]

Yes, because from the last test tube we discard that 1ml

[@gilbertkapapa5282]

Nice

[@mazrinamazlan1838]

Hi there I have a question let say I have very high absorbance reading so i dilute for 1:10 and then 1:100 how to calculate the absorbance? Let say at 1:10 the reading absorbance is 2.5440 so what is the exact absorbance?

[@Nowolf]

absorbance might not translate directly from the original solution to the diluted one. but you can use the diluted absorbance to find e,g, a concentration, and the concentration of the original is explicitly 10x that.

[@daliakhater2558]

cool

[@harry011984]

It can't be 1:10 as there would be 11 parts and you have 10 parts.

[@Pauliwog13]

that is what i thought too

[@timate9370]

Interesting, ich glaube du bist deutscher (:

[@kameleonfn]

Thank you so much, it was so easy to understand