Using a pH Meter
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- čas přidán 15. 10. 2012
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This video demonstrates the proper use of a stationary pH meter. The parts of the instrument, calibration with standard pH buffers, and sample measurement are shown. This activity is included in the laboratory textbook Biotechnology: A Laboratory Skills Course.
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1- Be careful with acid and base materials and wear gloves when dealing with them.
2- Handle carefully during inter-or intra laboratory transport to prevent breakage.
3- A pH meter needs to be calibrated daily with the help of standard buffer solutions.
Maintenance :
1- Store sensors at ambient temperature
2- The electrode should always be stored in an appropriate solution( KCL solution)
3- If you have a problem, tell the authorized person.
Cleaning of the pH meter itself:
1- The outside case of the pH meter should be cleaned using detergent or disinfectant (e.g. 70% ethanol) if required.
2- The area around the pH meter should be kept clean and any spills of samples or reagents cleaned up immediately.
3 - Any build up of salts on the top of the electrode near the filling well (if applicable) should be cleaned using water.
4 - All wires and contacts should be cleaned to avoid corrosion
5 - After the cleaning stage, rinse the electrode in distilled water and replace the internal filling solution (KCl) if appropriate.
Monitor the temperature daily as per following procedure. 5.2.5 Temperature shall be recorded which is displayed on LCD of the controller of the incubator. 5.2.6 Observe the temperature shown on the digital display. The temperature should not differ by + 2 °C.
Do I need to calibrate it daily if I don’t use it daily?
❤❤
Wow that's a really helpful mate
Many thanks but if you publish a calibration video and explaining the control panel component will be a huge help.
Transcription and translation into portuguese
00:08 - Medidores de pH podem ser pequenos e portáteis ou maiores e para uso em bancada.
00:12 - Nesse vídeo utilizaremos um medidor de pH de bancada
00:17 - As partes de um medidor de pH incluem o medidor, o braço e o eletrodo.
00:29 - O braço onde fica o eletrodo pode ser facilmente deslocado para cima e para baixo de maneira a possibilitar a inserção do eletrodo na amostra a ser mensurada
00:38 - Previamente ao seu uso, o medidor de pH deve ser calibrado em tampões (buffer) cujo valor de pH é fixo.
00:44 - Aqui ilustramos tampões com valores de pH de 4 (mais precisamente 4,01), pH 7 e pH 10.
00:54 - A primeira etapa de uso do medidor de pH é remover a capa protetora do eletrodo.
01:00 - Essa capa é necessária para que o eletrodo seja armazenado de maneira apropriada, imerso em uma solução de conservação e evitando que ele fique seco
01:10 - Depois da remoção da capa, o eletrodo deve ser rinsado com água destilada.
01:24 - Cada medidor de pH apresenta o seu próprio procedimento de calibração, apesar de que, a lógica de calibração é similar para os diferentes equipamentos. Quando for utilizar um medidor de pH pela primeira vez leia o seu manual para verificar como deve ser feito o procedimento de calibração.
01:36 - Para este equipamento a calibração é iniciada no tampão de pH 7,00. Aqui basta pressionar o botão “Cal” para entrar no modo de calibração, depois imergir a extremidade do eletrodo no tampão 7,00. Depois disso o botão “Cal” deve ser pressionado novamente. Quando o aparelho indicar que está calibrado para aquele tampão, retire o eletrodo de dentro do tampão e rinse com água destilada, a qual deve ser coletada em um béquer.
02:15 - O mesmo procedimento deve ser realizado novamente, mas agora com o tampão de pH 4,0, inclusive rinsando o eletrodo com água destilada ao final.
02:45 - Finalmente o equipamento deve ser calibrado com o último tampão, de pH 10,0, por meio do mesmo procedimento já descrito anteriormente.
03:06 - O medidor de pH deve ser calibrado sempre que for iniciar um procedimento de medição de amostras.
03:18 - Quando você finaliza a calibração, o medidor de pH está pronto para medir o pH de uma solução qualquer, conforme será exibido a seguir. Cuidado para não pressionar o botão “Cal” quando for realizar a medição de amostras. Posicione o eletrodo dentro da solução a ser mensurada, da mesma maneira como foi feito para os padrões. O valor de pH mensurado para essa solução foi de 5,57 para uma temperatura de 23,9°C. Para finalizar o procedimento, rinse novamente o medidor de pH com água destilada e coloque a capa protetora do eletrodo.
Is this the same PH meter device and technique used in QC departments of pharmaceutical companies? I am applying for a QC position and want to get familiar with some lab tests such as pH meter, viscometer, FTIR, HPLC and GC. Please advice!
Hi, what amount of volume (minimum) would the pH meter be able to read?
I was trained to stirr the sample while the pH meter was measuring. Is it not necessary? And why? Thanks
Hello, this is a nice video for introducing new students to a pH measure routine! Congratulations!
I need to measure the pH of a sample that (I expect) has a pH
Yes you can check this video for more info regarding this question: czcams.com/video/dBtcu6z_GFg/video.html
Very clear and easy to understand 👏👏👏
Can it be used to read oil pH? Like automotive oil pH?
If so, can read it as it is? Or it requires to be Ia solution of ethyl alcohol or so?
Thank you
Hi
I need to learn frome the BOD test
merci
This is the question for my assignment:
if you had the choice between a 1-litre beaker and a 1 litre graduated cylinder, which one should you use to measure volumes with maximal precision when making 1 litre of PBS?
I am actually not sure how to answer it.
How long should a pH reading remain stable for you to consider it as the recorded pH value?
How to calculate slope was not mentioned, not rinsed probe well
Very useful vedio. Thank youu👍👍
how efficient is ph meter to ph litmus papers
this is my first of many assignment to write on notebook
Mettler Toledo S20's the best pH meter ^ ^
Why you're using to 7-4-10 in buffer solutions
I require digital pH meter for blood bag pH measure ment. So please suggest
What is the logic and explanation behind rinsing the electrode before every use? Is that because not letting the electrode dry out?
Cross contamination, you don't want droplets of a ph4 solution in a ph10 solution when trying to calibrate
Thank you ✨
If I'm preparing a buffer and need to adjust the pH, can I just stick the pH meter into my buffer to check the pH? Or should I pour a little bit of my buffer into a beaker and test it there to avoid contamination?
Buffers are pretty resistant to pH- changes so u should be fine by just checking it.
I want to know in which solution is that electrode dipped to prevent it from drying out.
@@BioRadLaboratoriesCorp Thank you so much !!
I was trained to wipe water droplets from the probe's head with the help of clean kimwipe paper, each time after rinsing with DI. This is how I always treat probes. From one of your comments I realize that this practice may reduce the life of the probe. Please correct me if I am wrong.
u r wrong
am correcting u
Well, I saw experts doing it in both ways. You can always gently remove the excess water so that it does not go to your standard or unknown solution.
i am an expert and i know
Mamoon Rashid no it doesn’t , as long as you just wipe the outside and not the inside of the probe.
Are there any videos (or documents) showing how to fill the pH meter with filling solution?
Thanks!
this video was very useful for my exams
If that is a SevenEasy ph meter can you tell me which slope to use as the calibration slope. When doing a 3 point calibration it gives a slope from each calibration point, is the final slope read after all three calibrations and then when you check the calibration in the 7 buffer?
Isnt distilled water acidic?
Calibrate daily? I plan on only using it once a month.
Can you use ph meter to check plant extract ph ?
PH=-log[H+]
PoH=-log[oH-]
[H+][oH-]=1×10-¹⁴
[H+]=10-pH
[oH-]=10-poH
[H+]=[strong acid]×[liberated moles]
[oH-]=[strong base]×[liberated moles]
Interesting
Will the pH of the solution change if i will add 50% of distilled water into the buffer solution of pH 8
Thanks so much
We maked some tests on the laboratory and notices that a solution can be diluted up to 10 % with mili-Q grade water and the pH will no change.
why temperature is important
I have exam tomorrow 👌🏻
Show!!!
How many(USD dollar) cost pH meter?
Do you need pH meters?
Buenas tardes por favor quisiera en español
thank sir
vamos tilin
👌🏼
4_7_9.2
منو من المعهد يبااا
👌 👌 👌 👌 👌 👌 👌
Ohh
YEAHHHHHHHHHHH
is the unknown solution...mixture of all the different ph solution??
+BioRadLifeScience hello...and thanks for the help... really appreciate your video...👍
NUCOMers🌚? Where r u people?
For those of you that doesn't know what a pH meter is, it's basically.. Wait I dont know what is this shit
🌼🌷🌼🌷🌼🌷
I’m Tina ans I’m watching this bc my teacher forced me to! Btw if ur from my class comment 😁
What class?
And school?
Aryan why do u need the info
And also we are also forced to watch the video
Idk just curious
It's Shrek. D:
Mr k
Hello guys
.
💀
Yoo aryan ☠️
the 69th comment.......
Thank you
Thank you
Thank you
no thank on my home