How To Grow Unlimited Mushrooms With Liquid Culture!
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- čas přidán 9. 09. 2024
- UPDATED VIDEO HERE:
• Propagate any Mushroom...
In this video i demonstrate how to make a liquid culture of any mushroom species using Karo light corn syrup. This method is bare bones intro to liquid culture. Showing the most basic entry to grow mushrooms.
EDIT:
Use PAPER micropore tape, not plastic micro pore tape. You may use a steam canner (2 hours) to sterilize your culture media instead of a Pressure cooker. using a pressure cooker sometimes will caramelize the sugars (bad) due to the high temp.
In this video i demonstrate how to make a liquid culture of any mushroom species.
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I have fresh liquid culture and supplies available at sporensprout.e...
Red rtv high temp silicone- Lowe’s/homedepot/any car place. Use this instead of the tape and it’s a self healing injector port
It's a beautiful thing to be honest
But doesn't this eliminate air exchange? I thought that was the point of using micropore tape
@@DAAI741 drill a separate hole for either poly fill (have to be careful not to get that wet) or look up hydrophobic syringe filters and google that with mushroom and you should find the size you need and videos on how to drill and attach to canning jars
Out of all the videos I've watched on liquid cultures this answered all my questions and was the simplest to follow, thank you! 🍄🌱
No problem! Thanks for watching, Check out this updated video!
czcams.com/video/JYl_UnYK_kU/video.html
I love that u simplified things with a single hole. Not ideal for larger scale production but so simple for the small home farms. Thanks
Would have been great if you showed that 2 or 3 week later image of a successful jar at the very end.
yes that would have been a good idea. I have a video of very think growth from this technique i can show you.
@Somsouk Sourivong www.etsy.com/SporenSprout/listing/857369035/oyster-mushroom-liquid-culture-syringe?Copy&ListingManager&Share&.lmsm&share_time=1603999711267
@@themyceliumnetwork what?
@@themyceliumnetwork Well if you buy 7 it’s free shipping I think
@@themyceliumnetwork I see, I think I have free shipping for international turned off. I can turn it on for you
Don't mind me....Just gonna binge watch your channel while i keep prepping more seed spawn.
Thanx for this affordable L.C. video. Thank you for not including expensive ingredients. It's demoralizing to me when the video lecturers tell me to use costly ingedients or tools, like a Nasa space laser thermometer, or a calibrated P.H. meter that costs $200.00. You did great here.
That’s because they are trying to sell you their products as well , not just mushrooms or spawn etc , it’s another way for them to scalp more money from you the customer. 👍
Funny I realize I actually bought my initial spore syringes from this channel and didn’t even realize until now. They’ve worked great!
Thanks for the support! 🍄 🙏
@@SporenSprout after injecting the jars where do you put them to grow and what temp ?
@@strictlyundergroundhiphop5129 on a shelf in a room that is around 75 F
@@SporenSprout in the dark ?
Best tat ever!!
🤔
I admire your patience, the amount of already answered questions (particularly the marble confusion 😆) is staggering. Thank you for the information.
😂 Thank you for watching!
Wow.... this is a very good idea to have a plethora of any type of species on hand at any given time. Thanks for the knowledge.
I'm so glad to fall down this rabbit hole because of this video....
I have lots and lots of questions because I am in the first phase of broke boi Tek, about to move into the monotub. I subscribed for all!
Great Video. Turned my bad attitude into a good one. Best vid for LC.
Thanks for watching ! I’m glad it was able to brighten your mood, good luck my friend! 🍄
I’m a newbie at this, so thanks for the instruction…very clear and helpful! My questions: 1) Where/under what temperature and light conditions should I store the jars? 2) For how long can the jars be stored before going bad? 3) Can you use liquid culture (mycelium) from a syringe as well as the spores within a syringe? Thanks!
Thanks for watching! Store colonizing jars at 70-80 degrees light doesn’t matter but darkness is preferable , once full they will last for 6 months at room temp and can and should be refrigerated to force the mycelium to go dormant for longer than a year.
@@SporenSprout Awesome, thanks. How about “liquid culture” syringe vs “spore” syringe?
@@jeff042854 Liquid culture is just spores that have been germinated by introduction to sterile nutrient media. Spore syringes are made with only water to prevent them from germinating, they can be store for years before use.
@@jeff042854 Also a spore syringe contains many different strains of the species. A liquid culture syringe is one strain. So the genetic differences will be wide with spore syringe.
@@SporenSprout I did broke boi Tek about 2 weeks ago. I have vermiculite ready for the monotub with all the alcohol to get it ready.
I took 11 cc's and divided them between six bags. Some of the bags look like they're about done and some look like they're about halfway done. How do I know when to move the cake to the mono tub? Should I do each one as it's ready, or do I wait till they are all done? I'm going to be watching your videos because this is the first one that I've seen, but if you can answer my question I would be very much appreciative for it. They are already talking to me, I'm excited!
NOTE: If there is more than 4% sugar to water ratio the liquid can be come toxic to the mycelium/spores. It's better to use less than more if you decide to guesstimate.
Agar Is much easier to trust.
What's the minimum sugar concentration?
@@contambrah If the maximum is answered above, isn't the minimum, by default understood to be ZERO - but with no food = no growth. Anything greater than ZERO will allow for reproduction.
Also keep in mind that with less food available you will also be triggering rhizomorphic growth.
@@somethingforeveryone4206 isn't that what we are looking for ?
Really great information for a newbie like myself. Thank you for taking the time to pass on your wisdom. You have given me the confidence to give this a go.
👆🏻👆🏻👆🏻👆🏻 Hey look up the handle for good spores,grow kits ,shrooms etc
Hello, I love your straight forward teaching style. Thank you. Question: could I follow your spore print method and then directly inoculate jars as you have in the above video?
Thanks! Yes that would work perfect
Its best to use liquid culture as spores take up to a week or so to turn into mycelium and spores are also usually contaminated so its best to do a brf tek with the spores, get agar, take a piece of clean mycelium from either the middle of a cake or the middle of a mushroom or something and place it into the agar, once it turns to mycelium you can use that to create your own liquid culture, you have a much greater risk of contam and contam is very hard to see usually in liquid
I swear you can find absolutely ANY tutorial on YT !!!
CZcams is awesome!
@@SporenSprout Just added two items to cart and favourited your shop as well 👍
@@sptheartist1803 Thank you so much for your support!
Great work. Very sterile and described each step very easily to understand🤟
I love cheap DIY mycology! 🍄❤️
hey im back again i didnt even realize this was you i watched your channel since the beginning and didnt even know
Thanks for watching! 🙏
I will say that my current jar of liquid culture was made with no magnetic stirrer and no marble and the entire thing is evenly cloudy. I just spun the jar with my wrist at least 1x per day for the past 12 days.
Where do you store the jars when doing this process for 2-3 weeks? In a cool room with indirect sunlight? Then fridge to store long-term?
@@jasdipsagu3952 Yep. High in a closet, probably ~71 F.
I agree with the comment below. Great, straight forward style of teaching. Thank you for posting this
I appreciate that, thanks for watching!
Thanks alot man. I rarely comment but I feel you did a great job. No bullshit just information necessary. Do you feel the stirrer was worth the extra cash
Yes! Best thing iv ever bought for this hobby! I think it was like $30 on amazon. As others have commented the stirrer actually brings fresh oxygen into the mixture and speeds healthy growth!
@@SporenSprout great ! Thanks for the info.! I will get one since I'm about to give this a shot.
@@bradpotetz9844 sounds good! If you need any help lmk!
Q1. Can sugar syrup made out of crystallized sugar be used instead of corn syrup?
Q2. What can be be done if i don't have a magnetic stir? What can be done to instead of that process?
Love 💕 from India
Yes you can use any sugar source you have available. You can just put a glass marble inside the jar instead of magnet.
@@SporenSprout put marble and shake the jar to stir it?
@@nitinroy1331 don’t shake aggressive but yes
Super straightforward no fuss method. Thanks! Some newbie questions:
1. How do you know when the mycelium has grown fully in the jar and ready for use/storage?
2. Can you just throw them in the fridge after for storage? If not, what's the shelf life like before they go bad (and how do you know they've gone bad?)
3. How does the Karo light compare with other mixes with malt extract / dextrose?
1. It is not definitive, but most aggressive species will completely fill the liquid culture jar with mycelium. Once the jar is full of thick mycelium it should be stored in a cold room or in refrigerator. Some species like lions mane and shiitake will usually only grow half way in the liquid culture jar and are not as aggressive.
2. Storage at room temp is fine for up to 6 months, refrigerated or cold room shelf life is over 12 months. It’s hard to tell when it has gone bad, but a way to test it is to put a drop on agar plates. If it grows it is still viable. If the culture is very old it will be noticeably slower or yields will be smaller and smaller over time.
3. Honey is way better than karo in my experience. Karo produces more thin, weaker mycelium and honey produces very thick, aggressive mycelium. It is good to switch between honey and LME once every 1-2 years to change up food source.
Check out my updated video about honey liquid culture: czcams.com/video/JYl_UnYK_kU/video.html
How some species produce different color mycelium in liquid culture: czcams.com/video/9D2X4f7PncE/video.html
Do the syringes or the jars need to be refrigerated at any point, or can they be stored indefinitely at room temperature?
The European company i bought spore syringes from say refrigerate is best.
I'm from India
This vdo is very useful
Thanks
Baby bottle tek was a great easy 1. The vid got taken down long time ago... up t use what you want. I like organic honey. I prefer it over karo.. helps not to use too much!
This infinite food glitch is wild
Super helpful. Thanks for you r info! Could maybe make an injection port with high temperature gasket silicone too!
Yeah this is the bare bones way to do it.
Here’s the updated video !
czcams.com/video/JYl_UnYK_kU/video.html
I'm your 700th subscriber!
Thank u this knowledge i will be using it
Thanks for watching!
I saw a guy make a hole, then bead liquid high temp gasket goo over the hole to make a self sealing inoculation point. This could use that to improve it even more
Yes I made an updated video here czcams.com/video/FZPObmBQ3Ko/video.html
If you invert your lid and put it in a hot oven it will melt stuck to the ring. That makes it easy to remove when you inject spore solution.
You should've put aluminum foil ofer the jars in the pressure cooker
You are correct
Yeah.......duh. Everybody knows that.
INFECTED PUDDLE stfu, not everyone does...if you don't have anything positive or helpful to say why say it?
Awesome info man!!! I know you said these are for LC syringes, is it the same procedure for spore syringes? Or do the spores need to germinate first? Thanks!
Same procedure. The spores will germinate inside the culture media and become LC
If your talking about making your own spore syringe then no it's not the same. You will need a spore print, and you would not add the sugar to the water. There's videos here on CZcams that you can follow.
@JonnyQuest [*Newb here*] what does the "LC" mean? (Trying to learn all I need to know and not just listening to one person so I have more of a non-biased, non-judgemental amount of info thats swayed either way)
I appreciate your response in advance😉
@@99thmonkeyresonance66 I think it means live culture? I’m not entirely sure myself also new
@@kamadavana5821 yes it does. Thanks for answering me. There won't be TOO to much here on "EwwTweb", about "growing mushrooms" in DIFF/ALT ways- (mainly- bc 'they', NEED you- to "buy from" THEM. Same goes w/ the "Gardening"/"plant industry's". Tbh, its DE-spicable- IMO.)
You REALLY, FACTually, DO NOT-
"NEED to buy, all the 'special stuff'- affiliated w/their 'operations'."
There are MANY, many diff ways to go about "growing your OWN mushrooms"- whether for medicinal/food/fun purposes.
A good place to start is the Reddit group! "r/mycology"- I believe its called.
Hope this helped.
So the "nutrient broth" that mushroom syringe companies use to grow mycelium for the syringes is just watered down corn syrup?
Yes or light malt extract
@@SporenSprout bro its very hard to get corn syrup in my country? Any other alternative that i can use?
@@Dykiboop yes many. Try honey, it’s better than Karo. Also light malt extract powder is good.
Subbed 😤 love the info
Appreciate it brother!
Hi, can I use the new liquid culture to make more liquid cultures without senescence or fruiting problem I use the third generation to inoculate substrate? ( I want to use it on cordyceps ); thank's
Thanks for making this video. How long can such liquid culture last for? Can you store it in the closet or long does in the fridge?
At room temperature they will last up to 6 months. Store in refrigerator for 1-2 years. Thanks for watching and check out the updated version of this video here czcams.com/video/FZPObmBQ3Ko/video.html
This is some quality content thanks for the help brother
Cool tattoo btw chemical structure of psilocin if I'm correct?
I really appreciate that bro! i will continue to post the most helpful content i can!
@@lachieperrem1188 Thanks ! it is serotonin but psilocin would have been way better lol
Brilliant! Thanks,
Can you do a video showing how you would extract it into a syringe that you could sell or give to a friend?
Yes I can do that. I’ll start working on it ASAP. In the mean time, quick sneak preview would be to tilt the jar of colonized liquid culture and inject empty syringe and suck up the culture that way. The only problem with that is, if your mycelium isn’t very dense and full in the jar it will be hard to reach. If that happens, try using LME recipe l (I will be making a vid on that soon too). Or you can super sanitize your work area and also use a UV-C light to sterilize everything and then simply open the jar lid and suck the culture up and close the lid right after. Hope this helps.
@@SporenSprout yes this helps but I’m also trying to figure out what syringes you purchase for this exactly like you order the syringes, extract the liquid culture and the replace the syringe with a stopper under a flow hood and then send a sterile packaged syringe to a buyer along with the stoppered liquid culture correct? Also when is your LC considered colonized enough to start extracting it? I have a weeks worth of lions mane LC that I stir everyday. When can I start extracting it? Should I wait a few more weeks of mycelium accumulation? And also how do I prevent it from forming hyphal knots?
@@HoneyandMyco order empty sterile 10 ml syringes from Medvet international (they come with needle attached) and a separate order of 18 gauge sterile needles, and syringe caps. Open the syringe package near flow hood or in SAB and suck the culture up, take needle off and replace with syringe cap. Now the syringe is ready to be labeled and packaged along with one sterile needle. That is all the friend or customer would need. One capped syringe of culture along with one sterile packaged needle. There is no set maturity to the Liquid Cultures. As soon as there is enough to fill the syringe with a decent amount it is good to go. Usually takes me about 1-2 weeks for adequate growth . Use a magnetic stirrer to insure they mycelium can be broken up and distributed for faster growth and also fresh air sucked in my vortex it creates.
@@SporenSprout thanks that helps a ton... I’m on medvet right now, there’s several types of 18g needles of varying thickness. Do you have a link to the specific ones you use?
No problem! Type in SYRINGE,10CC 18 X 1, LL, 100/BOX, EXEL in the search bar
Great clear instructions. You rock. Thanks again Ricky 💃🏼🕺🏼🎸🐈⬛😎✌️🍄
Very good guy!!
Thanks for sharing this vid; very helpful!
Richard File-Muriel no problem! I will have a lot more videos coming soon!
Amazing videos homie! New sub
very informative, thanks bro
Thanks for watching 🙏
Hi thanks for the video. I couldn't find my question in all the other comments, here it is: when storing the jars in the fridge do you put the jars in something like a sealable plastic bag to protect them from any nasties in the fridge, or do you just put the jars in the fridge as they are? Thanks
In a perfect world, you would have a dedicated mini fridge just for culture but to answer your question, I don’t use a plastic bag and I don’t know that anyone else does. If the liquid culture is super thick and full like it’s supposed to be, there is less room for contam also.
Love the video!
Thanks for watching!
Hi my friend I was just watching you on CZcams and I will say I was very impressed by your knowledge and willing to share with us. My son has been growing mushrooms for me to eat because of the research he has been doing about the health benefits of a few different types.... I have a question. We are in the process of making the jar myzilyume. We are in Utah so it's extremely cold. For two to three months his been trying to get some to grow. My question is how long will the myzilyume last in the jar before it's not good to grow anything. We want to be ready for when the weather changes and it's warm enough to grow them. We were trying for a long time and finally I told him to go ahead and keep the room really warm and let's see what happens. Two weeks later we were enjoying some kind mushrooms lol thank you
Thanks for the kind words! 🙏 mycelium In liquid culture stored at room temp will last around 6 months to a year and if you refrigerate the liquid culture it will last longer than a year.
Good Vid, clear precise informative presentation. Very helpful.
How do you recommend storing the new LC? Thanks
Is the tape you are using plastic or paper? Thanks for making this so clear!!
Paper tape is best: amzn.to/4awG7cm
Check out my other videos for more tutorials! This was only the first video I ever made.
That’s awesome. What do you think your contamination rate is for the jars ?
@@FishLubber Maybe 1 in 1000 jars and only from self error like accidentally reusing a needle or a failed injection port or tape becoming wet.
Great content😍
Thanks brother!
Quick question and I'm sorry if this has been asked: could I use a simple (sugar) syrup instead? I don't have corn syrup on hand but I do have sugar, water, and a pot. So I figure I'll ask!
Honey. Pure honey
Yes just use honey
Sugar will work
@@SporenSprout brother, do i substitute honey and corn syrup in a 1 to 1 ratio ?
@@crazygandalfwithweed1180 Yes
Hi thanks for this video! You said we could use a marble too. Does that mean we need the stirrer machine or would we just shake the jar with the marble in it?
You wouldn’t need the stirring machine if your using a marble. Just tilting the jar back and forth is good enough for the marble to break up the mycelium that will clump at the bottom.
@@SporenSprout what if it's a magnetic marble and oblong?
@@thersten Yes, just be careful not to shatter the jar.
Got a stir machine for less than $30. Its worth it. Make sure to put the stir bar in before sterilizing, but sanitize the bars first. I think they were the source of contam the first time.
Genius! Thanks. 🙂
Do I store it in the dark and at what temperature is best?
Awesome!
I appreciate if you answer my questions?
1. So after you do this how long do you need to let them sit and in what conditions until you can draw up the culture into new syringes?
2. How long can you keep the culture syringes and do they need to be in the fridge?
3. In order to grow fruit, will you then inject the same way you'd use spore syringes?
1. 1-2 weeks growth in dark 75-80 degrees.
2. 6 months room temp, much longer if refrigerated.
3.Yes, same as spores
@@SporenSprout Thank you
Fantastic video, thanks.
Huge fan and customer. One thing... you are using gas heat and alot of people have electric. Gas heats up faster. What would be the time in the pc for electric?
The time starts after it’s heated up to 15 psi so it would be the same time. I usually do 25-30 minutes at 15 psi.
@@SporenSprout ok. I just did mine. It did turn the solution yellow but did not crystalize. I added the mycelium to it. It just looks like i am collecting pee at the moment
@@SporenSprout one more question. Can the stir be ever other day or maybe 2 days?
I have a wild idea and I'm looking for some mushroom fanatics to tell me if (and how) it can be done. I really want to have lots of fly agaric in my garden. I'm looking for a way to make this happen. Should I try it with the method of this video? And what kinds of substrate should I use? 🍄 they are soooo gorgeous and magical! Thanks a lot fellow mushroom lovers 🍄🍄🍄🍄🍄
Amanita muscaria forms symbiotic relationships with many trees, including pine, oak, spruce, fir, birch, and cedar. You would have to plant the mycelium in a forest not in a garden.
@@SporenSprout thank youu!!!
I have had two starter kits for over four months and they both would not sprout. They are both very well covered with mycelium
After that long I would assume they are dried out. You should submerge the blocks in water over night and then set the hydrated blocks inside a shotgun fruiting chamber or mushroom grow tent to fruit.
Awesome Subscribed !!!
Thanks for watching! 🙏
Ty for video!
i love this vid this is so understable, thank you for doing stuff like this and i have one question - can i do this also with copelandia cyanescens culture??
Thanks for watching! And yes you can !
Thank for the knowledge ⚡️😎
Thank you 💚
Just a heads up to everyone a uk pint is different to other countries pints the uk pint is bigger don’t know how much I just rember that when I worked in a pub
I'm looking for video on what you do next in the process.
Make some grain jars and inject the culture into the grain jar. I have a video on how to make rye grain jars and also corn.
@@SporenSprout I appreciate your response and will peep the vid you mentioned.
When I did this, rust formed on the inside of the lid and dripped down into my jar.
Rust is not harmful to the mycelium but It is very ugly, you can switch to plastic lids instead of metal.
Great video man!
One question: after all that, what's the best way to "duplicate" the LC? Should I take a few CC from the jar and pour them in other LC jars? How many times could I do that?
Yes you can put a few ml in more LC media or you can put a few drops on a bunch of agar plates to multiply that way. There is no definitive answer to your question, it can be done many times until it stops performing well from old age, some species degrade quicker like cordyceps.
@@SporenSprout Thank you very much 🙏
Really great viedo, very nice and clear.
If I dont have one of these magnetic stirrers can I stir it up by swivelling the jar around with the marble inside?
Yes that will work
I could.not find marbles. Are stones ok to use?
Thank you for your response!
Ohhh you sell your own syringes. That’s cool. Ill be ordering some syringes then from ur Etsy
Sounds good! I have a wide variety of species available right now.
I wish you could of showed us the accumulation and collecting process?!
I have plenty of other videos showing the process
Mind directing me to one in particular? With a title?
@@josephrikers1111 Try this one:
czcams.com/video/EARNzNHAzf0/video.html
This one:
czcams.com/video/JYl_UnYK_kU/video.html
This one is good too:
czcams.com/video/MAcTxk085oM/video.html
What kind of micropore tape do you use because the one I used melted and came off during sterilization
It’s 3M paper/fabric micropore tape.
amzn.to/3Krhenj
@@SporenSprout thank you!!
Am I safe in assuming that, once your jar is colonized, you can then use THAT jar culture to make more jars of culture for a theoretically unlimited supply of liquid culture out of a single $20(ish) dollar syringe?
Yes that’s basically how it works
do you have a video on a good method to create a grow kit?
czcams.com/video/t55I21cyWYc/video.html
Awesome thank you sir, random question Is growing gray dove Oyster mushrooms the same process as growing lion's mane mushrooms?
Yes I would grow them in the same environment
@@SydThaRager yes
Can it be done with spors
Yes
@@SporenSprout thanks 😊 🙏
You will have mushrooms forever but you need to start your own generation(s)
The reason why you magnetic stir is there is no oxygen in the sterile liquid culture so stirring right after inoculation introduces oxygen to the culture as well as breaking up the mycelium to encourage growth. Great video!
hmmmm
How do fish survive in a lake without magnetic stirrers?
@@georgekatsinis5224 I would assume that wake, water falls, cascading rivers all help to provide oxygen as well as aquatic plant species, occasional erosion from cliffs or banks calves and this would also introduce oxygen.
@@SporenSprout I was skeptical about there being less oxygen in boiled water till I googled it and read up on tea related articles. It would make sense that when the magnetic stir bar is spinning the vortex it’s also pulling fresh air in the Jar introducing oxygen bubbles just like blowing throw a straw in a cup of water. I know when I spoke of it to other growers we all kinda had our hands in there air 😂 I’ve seen people not use any fresh air exchange and there LC was fine so adding the filter patch for fresh air exchange could benefit the final results. I know people who do use fresh air exchange for LC and people who don’t do fresh air exchange. It’s really up to the grower and what works best.
@@surefiremushroomsmicrogreens Awesome info! that makes sense!
Whats the next move after they germinate?
Watch the updated method here: czcams.com/video/JYl_UnYK_kU/video.html
Once you have the liquid culture you can do this:
czcams.com/video/qtVMbJf60Pk/video.html
my jars look really dense and cloudy with mycelium at the bottom only. is this normal or is it supposed to fill the whole jar? i stir every day and whenever i check, it all settles at the bottom.
Yeah that’s normal
Hello! Would there be any way to possibly spare a few mushroom caps and mushroom nutrients put them into a blender and use that mixture to grow more mushrooms?
Only if you are inoculating some wood chips outside in the forest or something. Look up the term “slurry” for growing mushrooms.
where can i get the liquid mecelom to buy any link
Sporensprout.com
I got mine from👇🏼👇🏼👇🏼
hes on instagram¿
Silly question but I’m a newbie … once you got your jars filled with liquid culture can I just fill empty syringe with the sweet goodness and just add to a substrate and get mushrooms … thanks for the video too !!!
Yes that’s basically how it works. Check my other videos and you will understand quick
100/4 ratio of sugars vs. water for those of you not familiar with the metric system.
@RUBEN_SHROOMS ON INSTAGRAM SALES QUALITY STUFFS What the fuck are you on? I don't ship anything.
At the end of your video! When you said! Do this for about at least once a day! How many times do you recommend daily? For those 2-3weeks intel the whole jar becomes Liquid Culture
At least once per day for as long as your using it
@@SporenSprout Radom question can I use organic golden light blue agave nectar rather then karo Syrup?
@@Paintingtube I don’t see why not. Maybe they will love it!
So why do i need an agar plate?
I could simply buy a LC syringe from the lab and make unlimitided amounts of LC. And if my LC contaminates i could rebuy a new syringe for 15$
@@spicypunch6029 Yeah that’s correct
@@SporenSprout so i could skip agar plates. But If i mess up and all My LC contaminats i have to Rebuy a syringe.
Probably very frustrating when all you jars are contamineted bc your LC is not Clean:/
@@spicypunch6029 Yes it’s best to make a couple agar plates as backups and for testing
how long do they last once you make the syringe??
Great video man! Is it possible to use honey instead of corn syrup? Like you do with agar recipes?
Thanks for watching! Yeah I use honey for all my liquid cultures currently.
@@SporenSprout thank you for responding so quick brother. I’m going to try this tomorrow, this changes the game for me. Mush appreciated
@@thegreenroomorganic No problem! Good luck with all of your experiments! 🍄 🧫
I like this video
Most liquid cultures are made from mycelium inoculated liquids such as your water and syrup solution , but spores from mushrooms I believe take much longer to develop into growing mycelium in a culture if I’m correct or put onto Petri dishes to grow first , how effective is spores alone from the mushroom on its own being injected into a grain to grow I have not seen anyone do this .
Amazing tutorial, man. I just tried this for the first time using my pressure canner and it ended up slightly yellow (caramelized) but not too much. Should i even bother inoculating it or just try again with a shorter cook time?
I think you should start over. I prefer using a steam canner for 2.5 hours. But you can do 15 psi for 15 minutes in your pressure cooker and it should be ok
@@SporenSprout Awesome thanks! I did 15 psi for an hour while I was sterilizing some grain jars but i realize now that they should be done separately. I think the two jars i overcooked cost about 14 cents in ingredients so its really not much of a loss. I appreciate the info and thanks again.
@@gabgerrard2045 No problem! Happens to everybody 😂
@@SporenSprout my sea level has canning in my area at 10psi. At what temperature should this pressure for?
@@Happyhippy70 at sea level should do same but 10PSI dangerous to go 15 at this level
Hey brother thanks again for the content. I have an extra LC jar of Cordyceps but I’m not ready to use it right now. I’ve been told cordy LC doesn’t store well but I was hoping to hear your thoughts on how I can make this LC last a few more weeks.
The best thing to do is keep it in a cold room. It will be ok there for 1-2 months.
@@SporenSprout thanks!
How do you store these? Thank you for all the great content! :)
Should be good at room temp for a few months, for longer I’d put it in refrigeration.
@@SporenSprout what's the max storage time in the fridge?
@@Kstorrs77 should be good for over a year
@@SporenSprout awesome thank you!
Amazing video. Does it work for every mushroom?
Yes
@@SporenSprout Cool, should I keep it in fridge?
@@jorgehabash8690 After it’s grown enough you can put it in the refrigerator
In the end, aren't you just diluting the one syringe? Thereby potentially getting low results from your diluted batches or needing to spend more time building up the culture from one?
No, the mycelium replicates.