How to calculate Km and Vmax values - Lineweaver Burk plot in Excel

This video explains about How to calculate Km and Vmax values - Lineweaver Burk plot in Excel.
Km and Vmax value calculation in excel from the enzyme kinetic data. Michealis menton constant (Km) can be accurately calculated from the lineweaver burk plot in excel.
Enzyme Kinetics: Lineweaver Burk Plot Explanation:
• Enzyme Kinetics : Line...
Related Videos:
---------------------------------------------------------------------------------------------------------------
Plotting Bacterial Growth Curve: • Plotting Bacterial Gro...
Colony Forming Units: • CFU: Colony Forming Un...
Serial Dilution Methods: • Serial Dilution Method...
Copy Number Calculation for qPCR: • Copy Number Calculatio...
How to analyze Primer Sequence or Oligonucleotide sequence designed for PCR / qPCR: • How to analyze Primer ...
How to generate qPCR standard curve in excel and calculate PCR efficiency: • How to generate qPCR s...
Real Time qPCR optimization, Calculating PCR Efficiency: • Real Time qPCR optimi...
Taqman Assay Vs SYBR Green Assay: • Real Time PCR Assays- ...
Resolving poor PCR efficiency: • Resolving Poor PCR ass...
Calculating molecular weight of Unknown protein • How to calculate molec...
Designing qPCR Primers
• Primer designing for r...
Oligonucleotide Preparation & Purification • Oligonucleotide Prepar...
How to check Oligo Concentration
• How to check the Oligo...

[@tamaradifc7566]

Thank you. We pay thousands in the college and my bio teacher does not even bother to explain anything properly. Thank you 🙏

[@manny_k2988]

Absolute lifesaver. Super straight forward video, thank god I didn't have to sit through a 20min video explaining 20 billion different derivations of the the MM equation

[@mlawliet6151]

You’re a lifesaver! I didn’t get how to do it _at all_ until I watched the video. Your tutorials are a gem 💎

[@allexis5803]

Best video of this topic I have found, I really couldn't understand this topic but you made it so much simpler to understand for my report! Thankyou, subscribed!!

[@amandaouk8962]

i have been finding an example of how to properly do it. Very straightforward and exactly what i was looking for!

[@abbiepalmer4547]

lifesaving video!! thank you so much

[@isaiasv9925]

man thank you soooooooo much this was so helpful needed to do this for a final exam and it explained what I needed to do so well thank you sir, may you have great days ahead of you

[@BioResource]

Glad it was helpful. Consider subscribing for more such videos. Thank you

[@user-bu6tk1mq7j]

absolutely brilliant video, thank you so much for putting this up! It was so easy to understand and literally saved me hours of work!

[@alphazenturbo]

Very straight forward. I needed to a L-B for Pchem and this was extremely helpful for making the graph in Excel.

[@BioResource]

Thank you. Consider subscribing.

[@montserrathermosillo780]

Thank you so much, your video saved 4 biology students today. Greetings from Mexico :)

[@BioResource]

Glad it was helpful. Consider subscribing for more such videos. Thank you

[@blabiblabiblabi]

Thank you so much. I did an experiment to determine Km. Now i know how do calculate it

[@mr.legend368]

Thanks brother....
I've seen lots of videos about problem but only ur video clear doubt problem

[@daniellerochagiro3476]

Thank you so much!!! This video just save my semester. GOD bless you!

[@BioResource]

Glad it was helpful. Consider subscribing for more such videos. Thank you

[@mojzarea]

Oh, Thank you so much, you life saver man

[@JJH2004]

THIS IS EXACTLY WHAT I NEEDED, THANK YOU SO MUCH!!!!!

[@BioResource]

Thank you. Consider subscribing.

[@LucasJensenOfficial]

Very well explained video. Thank you!

[@BioResource]

Thank you.

[@beehyve]

Explained it so well thank you so much!!

[@rosemaryjude26]

Thank you for this video. It was straight forward and detailed. You have earned a subscriber😃

[@kelvineivo]

Thank youuuuuuuu. You got no idea how much this video helped me .

[@BioResource]

Glad it helped!

[@dentedtester36]

Thanks so much, makes much more sense now!

[@michellemandelstam8633]

Thank you so much!!!

[@mariebrassart9868]

Very clear and helpful, thank you ! 😊😊😊

[@BioResource]

Glad it was helpful!

[@hewyin6130]

thank you!!! this saved me

[@axediepied6993]

Amazing work, I understood perfectly.

[@BioResource]

Great!. Consider subscribing for more such videos. Thank you

[@Not-Nur]

perfect video, thank you

[@daniel98carvalho]

Great video! Thank you very much!

[@BioResource]

Glad you liked it!

[@anaa7595]

THank you for making this video! IT was so helpful!!

[@BioResource]

Glad it was helpful. Consider subscribing for more such videos. Thank you

[@rawyld]

Thank you so very much.

[@katsuno5916]

what a life saver, explained it very well

[@BioResource]

Thank you. Consider subscribing.

[@fizakhalid8537]

thank you! this was so useful😀

[@aishwaryaramkumar1221]

Soo helpful honestly a lifesaver for my last minute lab report

[@BioResource]

Glad it was helpful. Consider subscribing for more such videos. Thank you

[@gud_soup6991]

Thank you brother, much appreciated 🙏

[@BioResource]

Thank you. Consider subscribing.

[@manonmclellan7304]

This video saved my biochemistry report thankyou!!!!

[@BioResource]

Thank you. Consider subscribing.

[@jl2584]

really appreciate your work!

[@BioResource]

Thank you. Consider subscribing 😊

[@samanthac323]

This was so helpful. Thank you!

[@BioResource]

Glad it was helpful.

[@GrayGhost_CV-6]

Thx sir, this so helpful for me

[@user-nt2jf7oc2i]

Saved my life man 🙌🏼🌷🌷

[@BioResource]

Glad it was helpful.

[@aqsaqureshi3833]

Mine too

[@Koeliaify]

The explanation is really very helpful

[@BioResource]

Thank you. Consider subscribing.

[@concurseiroentropy4483]

Very nice!

[@wei2183]

very useful ,thanks a lot 非常有用 謝謝你

[@katecherieandreallo8874]

So helpful ! Thankyou!!

[@BioResource]

Thank you. Consider subscribing 😊

[@maxw5900]

Thank you, Good Sir!

[@BioResource]

Welcome!

[@captainlux409]

Best explained video, thank you sir!

[@BioResource]

Thank you. Consider subscribing.

[@rspeks9461]

OMG thank you so much!!!

[@rspeks9461]

Got me through my chemistry assignment!

[@nikitarashinkar1897]

Thank you 😊

[@DeStIiNix99]

Thank u sir 🔥

[@kaoutar8656]

you are amazing

[@khadijahahmed1970]

Thanks mate

[@rakshamukhia7970]

Sir, I do not understand but Iam gettingthe equation plotted in the graph with a ngative sign. where iam I flawed? y=10.192x-0.3494

[@danielhuelgas5355]

Thanks a lot!

[@BioResource]

Welcome!

[@justflow7769]

thank you so so so so much !🥰

[@BioResource]

Thank you. Consider subscribing.

[@songulates7833]

Thank you so much but i didn't understand , why is backward=0.5 periods for forecast?

[@berkayadem7197]

thank you very much

[@BioResource]

Thanks. Consider subscribing 😊

[@zaidmaniar2899]

how did you get the initial velocities/product formed ?

[@usmanabdulkareem7699]

Nice

[@malleshgampa1956]

Substrate concentration we have to take in mM?

[@brianbridges8124]

hello, why do you bring the line to - 0.5, im wondering how u know what vmax is?

[@aelafalzabede6064]

4.3002 how i can result this?? i dont know 😢

[@zaysiz]

Sometimes, I get Lineweaver-Burk equation as y=ax-b. While 1/Km is possitive, 1/Vmax is negative. Is it problem? Why is it negative?

[@misbahhusolli]

I found difficult when use this plot for bacteria fermentation kinetic, where the curve of 1/s and 1/u are not the same as the literature. the line of the curve is downward, Y = -ax + b, but the R square = 0,9 kind of. what if the result of this plot is downward curve, is it still can be use as a data to calculate Km?

[@ximena5063]

No

[@zzziii-qr5io]

Why choose backward 0.5?????, can someone explain

[@foreverninteen8580]

What is the unit of substrate concentration [S] here?

[@solomonabioye1516]

Thanks for the video but I still need help pls

[@deepaksshanawad488]

How to calculate product formed values?

[@hajrakhalil6737]

Ak numerical solve krvna ta

[@handlelector]

2:15