How to Make Agar Plates for Growing Mushrooms
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- čas přidán 21. 05. 2017
- In this video I show you my process for making sterilized nutrient rich agar plates for growing mushroom cultures.
Get the full article here: learn.freshcap.com/growing/ag...
Learn More About Growing Mushrooms: learn.freshcap.com/growing/
Parafilm: amzn.to/2LMh91Q
Agar Powder: amzn.to/38f1SOz
Agar Recipe for Malt Extract Agar:
For every 1000 ml water add:
20 g Agar Powder
20 g Barley Malt Extract
2 g Nutritional Yeast
Sterilize for 45 minutes at 15 PSI. Pour into plate in a sterile environment. - Jak na to + styl
Thanks for watching! Get the full article here: learn.freshcap.com/growing/agar-agar-so-nice-they-named-it-twice-5-steps-to-pouring-perfect-plates/ Have FUN growing mushrooms :)
Hey whats the best temperature to store your agar and syringes in your lab fridge
I have an exam tomorrow on Mushroom cultivation technology and I can't explain how helpful your videos are proving to me. Your explanations and physical demonstration are so easy to understand. My book is really annoying and I was having a hard time preparing for tomorrow so thank you for such amazing content. And writing this currently, I have already watched 10 of your videos.
Best editing and fastest presentation of information I've ever seen on CZcams. You are a true artist, Tony.
"Trifles make perfection, and perfection is no trifle. Genius is eternal patience." - Michelangelo Buonarroti
Wow thanks so much for the kind words!
I personally think this is second best to Mark Keith's tequnique. He just missed a couple of important notes and tips. Agar solidifies at around 120°f and pouring as cool as you can reduces condensation. I'm also not partial to how he handles the dishes after they have cooled. The insides can be assumed sterile but to really go the extra mile you could place them in a sterile container before shutting the flow hood off. You likely aren't going to pressure cook a tote to put your petri dishes in, and sanitizing a tote is second best. So where do you find a sterile container suitable? The bag they came in is sterile, so if you roll it up and place it under your rack, once your plates are set you can put them right back in the bag, then you dont need to waste the parafilm and dont risk drying out your media. Is this technique straightforward and to the point? Yes. It's very professionally done in both style and production. Please take this as constructive criticism, I'm not bashing at all.
Mason Lundy great, great tips. Thank you so much!
@@FreshCapMushrooms I was wondering if you would be able to do a video on how to spec out and make a 24x24 flow hood and maybe a parts list. I know you did a video on making a 24x48 flow hood but I just think that's to big and expensive for the stage that I am at right now. Side note, I live your videos, the first one I seen was the bucket tek on and that just got me so interested in growing mushrooms. Thank you again for all the info and videos you do. Keep up the amazing work. I don't know if you coild maybe email me the instructions if you don't want to make a video. I'm finding so much information on CZcams and it is kind of overwhelming and I don't know who to believe and follow hence why I'm asking you I feel that the knowledge that you have in the videos that you have done so far have been an amazing help to me so far
I like how the dog looks through the window. Very calm.
dude, where have you been all my life? i've been learning mycology for a while now and had so many simple yet significant questions that all other online sources have failed to answer until now. thank you and keep up the good work! i'm so happy to find someone willing and capable to include the attention to detail that is so necessary to succeeding in this field. i will be recommending your videos to all my noob myco peers, and hopefully before too long i will have developed the skills and confidence to share my own wisdom via quality video tutorials like these. respect :)
How does someone get into mycology? Sounds interesting
Multisanchez.. have an interest for starters.
Bill Palmer of course, of course ^^'
ممكن لو سمحت تعطيني بعض النصائح انا ماعم أنجح ابدا
@@sanamahmod8610 ask the right questions
You are so wonderfully informative. By teaching the ignorant like myself, you are changing lives. It takes a community to save the world, a great start.
Best tutorial I have seen.
Accidentally had my playback at 1.5 he was very up beat
My playback usually sits at x2.5 I got things to do, places to go, people to see. :P
heyou yea definitely I keep my playback at the max. Watch 4 videos in the time of 1 😂😂
😂
@@joshualowe79 what???? Duh..u uped my info intake by 4. Lol wow. I'd have a doctorate by now
This comment is too funny
Great video, very informative, quick and to the point. No wasted banter and you covered everything! Thanks
I have learned so much from you! Please don't stop teaching!
You produce the best condensed videos of lots of great information and cool little tips that made my life easier. Thanks
Thank you Professor🙏
Dude, your channel is awesome. Thanks for sharing so much valuable information.
Thank you for the great information! I've been using your agar recipe to grow oyster mushrooms and have had very good results. Also, I love your dog in the background...very stoic!!
Great job on the clip..
Very informative and clearly presented I will be trying soon. I need to make a flow hood .
Thank you for this video. I’m very new to all of this and videos like yours make the whole process less daunting.
Great video Tony. Very helpful and professional. We really enjoy your Lions Mane and Cordycepts supplement powers.
Thank you, Tony, for another extremely helpful tutorial!
Great video! Precise and great presentation. Just a damn solid video!
Your videos are the best out here, thanks!
Tony is the best guy, when it comes to disseminating Mycology Knowledge! ❤️
coolest job done tony loved your script best defined and executed.
Such clear instructions.Thank you!
Thanks so much for sharing your knowledge!!! I can’t wait 🤩
Great Video! As a passionate Home Brewer I can tell you that you‘ll find all the materials in good brew stores. Including petri dishes Agar agar and off course the rye which is used in the mason jars. These materials are also used for yeast cultivation.
Thanks for that info! Home brewing is something I'm learning about as well as mycology.
Gracias, buen vídeo claro, práctico y educativo.
I like your process. Thank you for sharing.
Man I love your videos. You give so much important information in a quick and easy to understand way. I like watching your videos as much or more than I enjoyed Roger Rabbits cultivation class videos that I had to pay for!
Awesome! Glad you like the videos :) RR's videos were gold- pretty sure he made those before CZcams was really a thing :)
roger rabbit can suck it (hence the lower case r's) I have always found him so arrogant in the forums.
Thanks, you couldn't have explained that any better. I wish more people videos would just get down to basics.
I learned so much . Thanks a lot for your valuable time. Thanks for Sharing your knowledge.
Excellent info! Thank you
Great technique! Getting good at handling plates is like getting good at shuffling cards.
Your the greatest👍🏻 always spot on explanations for us to try out our luck at a great hobby or possibly a business
AMAZING VIDEO THANK YOU!!
By far the best video on mushrooms👍
Another great video. 🙏🍄💖
I liked the video because i like your stacking while pouring technique seems like it will help my arms a lot. Also folding the parafilm is a good idea makes it a little stronger so doesn't rip if you pull too hard
Beautiful video! I wish i had a laminar flowhood like that one!!
still air box, from a plastic tub works well too
That's knowledge I'll take it. Thankyou
Awesome videos, thanks for sharing.
Thank you, that is very clear explanation. 😊
materials necessary:
barley malt extract -- brewery supply
nutritional yeast -- a nice grocery store
petridishes -- in example, pack of 25, online or lab supply
perifilm (to seal the petridishes), online or lab supply
agar agar - science lab type supply stores
scale for grams, one liter + measuring cup,
a bottle with good cap - 750 ml recomd. (to fit inside of a pressure cooker)
small funnel, polystuffing (for bottle cap)
pressure cooker
other typical lab stuff you already have -- scissors, gloves, masks, alcohol, and hepi-filter or alt.
Malt Extract Agar Recipe, for every one LITER of warm water:
20g Malt Extract 20g Agar Powder 2g Nutrional Yeast
useful for :
making clones, developing from spores, transfer culutures, making spawn, storing/ maintaining a culture bank
thank you so much this was so helpful
Great video! Subbed!
Short sweet and best information !! Thanks
Few questions
1) After inoculations, how to incubate these agar plates? Do this really require BOD incubator? Or any cost saving ideas?
2) How many times can we subculture these primary culture? Does passaging multiple times affect productivity?
Looking forward for your experienced guidance
Great video..great presentation
Love the background music
I used a pressure cooker for the first time today. I also made agar dishes and innoculated them for thr first time. First grow ever actually, hoping it goes well but if not thats why i chose to learn the agar! Thanks !
How are your grows going? I'm just starting agar work rn.
@markgarry7866 by now amazing man. Currently I have 3 LC's I work with TAT, PE, and enigma. It's my first time with it haha today marks 20 days since fc and it's going hard!! I highly recommend a flow hood if you can afford 450$. Lab rat hoodz has AMAZING hoods for the price, but they take like 4 months to reach your doorstep after ordering. 2 man operation build on order... but hey, keep up with the agar. I use to do spores, but ITW has amazing LC. I grew that out and cloned what came from that. All in all tho, my grows are fucking fantastic now... stick with it, try things... if you ever struggle with trich just add 50g lime to bucket tek boom no more trich
Thank you for this! keep it up dude!
Thanks for watching! :)
FreshCap Mushrooms waiting for new videos 😊
thanks for this video , lots of information
Happy you liked it :)
NICE WORK!
Excellent video.
I recommend using a canning jar, not a whiskey bottle. If you must use a bottle, choose one that has rounded corners at the bottom. If it has an abrupt transition from the walls to the bottom, it is much more likely that the bottom will break off the bottle when heating or cooling. An abrupt change in the thickness of the glass could also be a problem. The ideal container for heating or chilling is either borosilicate glass or spherical in shape or both. (Chemists use round-bottomed flasks.) The closer your bottle is to this ideal, the less likely you'll lose your bottle and contents in the pressure canner.
BTW, pressure canners are moderately common at garage sales and flea markets. I've bought two this was and passed up many more. Not all of them are as large as Tony's seems to be, but I've never seen one that couldn't handle seven 1-quart jars.
Hola podias subtitular, es tan interesate lo que mostras de los hongos. Yo cultivo desde hace 20 años. Pero siempre miro video para aprender mas. Gracias.saludos desde argentina!
I would like to learn this to save money and have as a hobby. The whole process is super cool
Great information Tony. How long will the agar keep before it’s not useable?
Very good and clear explanation, more video please!! ;) Thanks for making this one! ;)
Awesome, I'm glad you found it useful! I will be working on many more videos in the near future.
Awesome!
Your videos are the best on youtube for mushroom growing. Im sorry to point out that u didnt mention putting water in the pressure cooker. Ive never used a pressure cooker and almost started mine without water. I went back to the part in the video where you put the bottle in the pressure cooker and saw water in the bottom. I love your videos and pray for more content from you, im just worried that a newby may not add water to his cooker... love your work.
It's like instant noodles, just add water.
It's all so easy when you get beyond the difficulty.
the music on point as usual
Haha thanks!
Parafilm is the coolest material on earth. I used to LOVE playing with that shit when I used to work at a microbiology lab.
this is cool work thanks
Glad you like it thanks for watching!
Your videos are soooo good
Tony you're awesome; I just subscribed! Very clear and well-paced. 👍🏻
Question, can you use a different medium than agar?
For instance, gelatin, or carageenan?
NO. You're better off using the agar. Gelatin is an animal base product (collagen) and has a thin consistency unlike the plant-based agar. Also, it requires refrigeration to stay firm. so it's not a good replacement. I also heard that it can dry up very easily causing it to fail. I would think that mushrooms prefer the plant-based medium anyways.
Hey man love your work. Both your videos and the very well organized blog. I am trying to make agar plates for ultimately home grown yummy mushrooms using coffee grounds. I would study your content. Also I would love to know what wordpress theme (or what tool) did you use for the blog. I love the side index that moves to scroll.
TaThank you from thousands of miles away for the tutorials
Thanks, I was looking for some pointers on how to apply the parafim tactfully.
For brewers out there, 20g of dry malt extract per liter is about 1.8°P or 1.007 S.G.
Basically, you could take your final runnings and water it down to 1.8°P or 1.007 S.G. just add the 20g of agar agar and nutritional yeast, and your good.
Thank you
I have that same Tupperware funnel lol don't know why that makes me so happy lol
you rock you are my sensei
Nice thank you
Such a great video. Can the actual plates be cleaned out and reused later for fresh agar?
Great videos! Just wondering if you ever plan on doing a liquid culture video?:-)
Thanks for the awesome vids!
I am struggling to find malt extract, I have collected everything else but that. Ive been looking all over. Please tell me if and what substitute I could use as a replacement ?
Nice! I´m gonna buy some whisky just to try this in a couple of days after I´m done with the whisky and with the hangover. XD
Hello Tony . I like your videos ! Very informative !
Can you plz provide the model # for that fan on the 24x24 flow cab ? Thank you !
Thanks bro 😎
Hey Toby, I just love your vids, can you put the agar in the petri dishes and then sterilise the petri dish??
great!
One thing I use is the grain water from simmering is used to make my agar plates instead of plain water
This is really really well presented.
Ryan Prince thanks!
A whiskey bottle? Tell me you're from Alberta without telling me you're from Alberta...
Love it!
in our lab we would autoclave the agar in the dish. that is much easier and contamination is minimized than pouring into dishes. just be sure to use glass dishes.
Great!
great video!! very informative..hey how did you build the laminar airflow
Thanks! I have a video on the flow hood, over on my channel, or you can just google "freshcap laminar flow" and it should bring up my page on the subject. Hope that helps!
Awesome video, question: where i live the only barley extract i can find is in liquid form- dark color- looks like maple syrup. Would that do the trick or does it have to be powder? Cheers
Amazing content but I have been getting contaminations do I need to sterile the paralfim I do it in still air box
This is the best explanation ever seen Toney. I was wondering what I can use to substitute the barley malt extract and the agar?
Hey Nora, sorry for the late reply! You could can get agar from some grocery stores, and may be able to use potato starch or other sugars as a substitute for the barley malt extract.
I really learn lot's from you . Just setup a small laboratory for spawn. Is that bacteriological aga agar suitable for oyester PDA ? Plz suggest .
🎯 Key Takeaways for quick navigation:
00:02 🍄 *Агарные плашки - важный элемент в процессе выращивания грибов, их можно использовать для клонирования, выращивания культур грибов и многого другого.*
01:13 📋 *Рецепт агарных плашек: на 1 литр воды добавьте 20 грамм агара, 20 граммов солода и 2 грамма пивных дрожжей.*
02:19 🧪 *Агарную смесь необходимо стерилизовать под давлением перед заливкой в плашки.*
03:21 🍶 *Плашки следует использовать только после охлаждения агара и соблюдения стерильности.*
04:25 🧼 *Перед заливкой плашек следует подготовить стерильную рабочую область и стеклоочистительными средствами очистить их.*
06:11 🎥 *Завершите процесс, запечатав плашки парафильмом для защиты от контаминации.*
07:18 🍽️ *Готовые агарные плашки - полезное средство для выращивания грибов и проведения микологических экспериментов.*
Made with HARPA AI
Hey Tony, perf video! I was just wondering, the fact that you are drying your petri dishes with closed lids doesn't lead to increased humidity in the petri dishes and thus sometimes smeared cultures? Wouldn't it be better to leave the petri dishes in front of the flow hood like you do now, but with half open lids? I guess they should still remain sterile in such way, right?
Woah woah man, I know a sprain Morgan bottle when I see it
whiskey bottle.... love it!!!!
Thank you Tony for all the mycoinfo you’re sharing. I have some dowl inoculated shiitake logs that are slowly flushing for their first time. I have access to an industrial autoclave and lfh as well as YM (yeast/mold) nutrient agar.
Is this media suffient for growing shiitake culture? What type of streak or inoculation method do you prefer when transferring from spore print to plate?
To update, i picked my shiitake cap a bit too soon and didn’t get a good spore print. I had good success cloning from a cap and now have 4 quarts of healthy grain spawn and agar plates. My plan is to use the grain spawn to inoculate 500 dowels for logs this spring and start some fresh jars of grain spawn as well. I also have isolated a wild oyster (yellow?). It’s amazing how much faster the mycelium grows compared to shiitake. I’m going to inoculate substrate blocks this weekend and hopefully have indoor oysters this winter. Thank you Tony and Teegan for all your helpful videos and blogs!
How long do the agar dishes last? Do you put the tape on them after they agar has solitified or before?
nice, very straight forward but I have a question, has to be barley malt? are there equally good alternatives? and I have agar agar not from science store, works same way? thanks
how do you clean the bottle afterwords ? after the residual in the bottle solidifies...?
What kind of flow hood do you have? And how did you install it?
Did you make your own flow hood? If so, any plans that you can share?
does anybody know if i can use liquid malt extract instead of powder?