DNA Transformation into Bacteria

Anne Wagner of the University of Pennsylvania describes the transformation of E. coli with DNA coding for the green fluorescent protein (GFP) and expression of GFP driven by addition of IPTG to the growth media.

[@Boubavisham]

I am about to do a bacterial transformation for the first time - this presentation is very helpful. Thanks!

[@luwang2697]

Thanks a lot! This video is quite helpful! Especially I appreciate the cute illustrations :D

[@siddharthbania4583]

thank you and a wonderful video. hope that we get more videos. quite helpful

[@Drn00by]

Great video! I will be doing this myself shortly, and having no clue whatsoever - this video was quite enlightening. :)

[@gorgon825]

Brilliant video and speaker. Thank you. - AP Biology student.

[@guidaliad]

Amazing video!!! Thank you for clarifying things.

[@TheMedicalBoss]

Nice Video, Thanks Anne

[@YablonskaOksanaSevama]

Thanks for very interesting work!

[@martinbakht2214]

well done!

[@maleshamabotja6196]

great video,really helped

[@dangviet8758]

thank a lot ^^

[@DrAfeefaCh]

Informative

[@hosseinjamali3930]

Thanks for your helpful video :)

[@SocialTalkswithMeta]

this video is amazing. thnx for uploading it

[@annierichards7367]

Thank you for your video.

[@JayRileyArgue]

Great job.

[@lakshmi3002]

I could find that LAF was not used to inoculate/ plate the E.coli cells.

[@sannydavidlumayno9078]

thanks for this video! i'll be performing bacterial transformation later, now I clearly understand what I will be going to do.. :D

[@DanielAleksanderJensen]

What would happen if you took human DNA and inserted it in a microorganisms's nucleolus???

[@DevOpsSkills]

Thank for the good presentation. You are so beautiful.

[@FootyShorts510]

This dna implemented is plasmid dna right?

[@ravithakur4278]

small volume means how much???

[@Raberuji]

could also name this "superbug, an how to" ;D