Isolating Plasmid DNA
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- čas přidán 29. 08. 2024
- The ins and outs of plasmid DNA isolation. This video explains the how and why of each step of a plasmid DNA miniprep. Produced for Colorado College BY131 classes.
CAST
Narrator: Phoebe Lostroh
Biologist: Nguyen Nguyen
CREW
Director: Katie Wolfson
Writers: Katie Wolfson & Nguyen Nguyen
Camera/Lighting: Zach Wolfson
Camera: Meredith Mantik
Animation: Katie Wolfson
Music: Kevin MacLeod (www.incompetech.com)
Special Thanks: CC Biology Dept, CC English/Film Dept, CC IT Dept, Sarah Withee, Zach Wolfson, Ralph Bertrand, Terri Holzen, Nancy Huang, Darrell Killian, Phoebe Lostroh, & Mark Wilson
This is fantastic!! In 10 minutes, you've managed to explain something that my professor hasn't been able to do in an entire semester. Thank you.
"...remember to balance the centrifuge!" #1 message from this video! Great job everyone! I love the triumphant music and narration at the end - the plasmid DNA is isolated!
Amazing! I usually spend at least 72 hours of research to understand the purpose of every step explained here because of my failing professors and TAs.
Still relevant till today, thank you all.
This video definitely needs thumbs up... easy and straightforward steps...
You are too awesome Katie. The process is very clearly and enthusiastically explained - great animations too! Thank you for this it has been very helpful.
Johanna Amunjela Thank you so much! I am glad the video was helpful and you enjoyed it.
+Katie Wolfson amazing, they should include a link to this video on the QIAGEN kit :)
This is by far the best lab video I've ever watched! Usually all the vids I find are really boring. Thank you for making it interesting
What an exquisite way to explain the process of plasmid DNA extraction from a bacteria, the best I have seen so far.
the music really gives the forefront of human ability a different vibe. A+
I was totally lost while rushing through this procedure in lab. This video explained everything so clearly! Thank you!
I think this is great educational video out there. It shows simple process at first, and then goes over actual process explaining what is going on in detail. I find this really helpful.
A fun and educational video describing the steps of mini-prep in perfect detail. Thank you so much for this wonderful resource!
I really must stop and say that this video is so amazing, and I love it! I just would like to be more prepared to perform this in the lab for the first time, and this really has helped me to visualize the procedures! Thank you very much for your superb video!!!
THANK YOU FOR HELPING ME UNDERSTAND; your clarity and simple animations really helped me make sense of the whole practical aspect :'D
This was one of the most helpful videos I've watched on CZcams. Thank you so much!
I was searching this video all years long, extracting is mastered now. I DO NOT WANT TO SEE OTHER DISTURBING VIDEOS.
great video! couldn't have made it any clearer than this. had to leave a comment 'cause you guys did a great job!
Nice job on the video. The explanation was clear and concise. I would drop the music though. It was distracting.
by far the best on miniprep outline, explanation. This was an excellent video.
Honestly the only video that explained it good, thanks!
This video is very nicely done! I get the principle behind the technique now. Thank you for sharing this great video!
I have been searching the whole net for the for the rationale behind each steps and most of them are just steps without the theory behind. This is a great video. Helped me a lot. thanks!
This is a nice refresher. I haven't done a mini prep in years. 😅
Great video Katie...you've put in a lot of time for it. Excellent resource for undergrad class. Much appreciated.
You're welcome! Glad you like it.
Very good video. Easy to understand and informative! 🌟
That was so great! I study Pharmacy in Scotland and we're doing this on Monday and writing a report on it at some point so that was very helpful, thanks! :)
Thank u! U r a lifesaver! I'm a research tech nd was freaking out but I'm now sure I'll do it right tomorrow!
probably the best mol bio technique video I've seen. well done!
I actually enjoyed watching this video. So cute and informational!!!
ABSOLUTELY SO HELPFUL! Thank you so much for this!
Amazing. You are so awesome! I wish every teacher are like this.
Get confused with a miniprep protocol. Thanks for the understandable introduction!
wow, I just took over a project that involves plasmid purification, and this video is a perfect prep for that.
THANK YOU, you just saved my academic career
This video is super helpful! can you make one on the western blotting technique?
I like this thorough explanation step by step in this lab. Good video. Thanks
Super easy to understand when you put this way!!! Thanks
so nice and clear voice, amazing , explain the technique in a simple way...thanks katie
Thank you, it helps on understanding more detailed.❤
Great video . More videos like this that have techniques that we can put on a resume would be good.
A Life-Saver Video! so sad that my English is not good enough to explain how thankful I am :(
We are planning to produce another video after the holidays. Please let us know if there is anything in particular that you would like to see!
It all makes sense now. At long last.
thank you! this is the exact mini prep kit we use in the lab !
Dang, CC's raking in them views! Great video
Absolutely the best !
KATIE, ILYSM
This video was bloody amazing xoxo
Great video - excellent description and the cartoon visuals are just wonderful! Thank you :)
Omg! Finally everything makes sense.
I just started doing this in my lab! Super helpful. Thanks so much (':
this was fun, yes fun. plus i learned a lot without even trying. wow. thank you. this was so helpful.
Millions thanks to this video!!! You made my day
Thank you so much. this really help me with my lab report.
U'r the best ... going to get an A for my lab report.
This is the greatest thing ever. THANK YOU!
It was an amazing video. Thank you so much Katie!
@MrMelbournemarsupial Thank you! Glad you like it.
Really owesome video. You are a lifesaver. thanks a lot.
Thanks for the video, just about to do my first miniprep today and a feel alot more prepared for it :)
Pretty damn good quality video for it being 11 years old wtf
Great explanation! I really enjoyed your video, congrats! Kind regards from Barcelona.
Absolutely amazing
this is awesome. Keep it up Katie
Perfect explanation!!!!!
Top notch videos Katie thanks so much!
It's easy and clearly defined Thank you.
This alleviates so much stress cause they gave us a 4 minutes video which didn't even show half of this
outstanding video please make other videos, like RNA protein purification.
thank you for this!!! this is a very clear instructional video! hurrah!
thanks a lot Katie, it was very helpful.
a very nicely explained video
thanks for the wonderful concise video, explanation was so helpful in my lab report :)
Hey, Thank you very much for this clip. It is a great video explanation and very useful !!
Super helpful video! Thank you so much!
Great video - more please
Thx alot for this tutorial....
thank you so so so so much!
Thank you so much for this! This is such a great video, the instructions are clear and thorough! Could you please do one on Western Blot as well?
Thanks! We appreciate it.
This is perfect.
Excellent Job..
thank you verry much.
This is so esay to learn .Thanks a lot
Great video!
Omg great scheme to explain the action of silica. :o Can I use it ?
MrJejayologue Yes! Please feel free to use it for education. Glad you like it!
Thank you so much! My school be like : read the manual...
Great job! is very clearly!!! thnks!
Sorry but I didn't understand this: For example, potassium acetate lowers the pH. Why does this only renaturate the plasmid DNA while the chromosomal DNA falls off?
thank you for such a beautiful descriptions........
thanx dear teacher
very clear
excellent video
REALLY THANKS. Just a question. How quickly should we work? how much allowable time between the steps is? can I do this procedures for 20 samples?
You just saved me a whole lot of trouble!
Thank you Katie.. very simple and innovative demonstration.. can i use this video to explain the protocol to my students...?
You are amazing... Dankeschön
fantastic video .. thanks
Good video, however, the function of acetic acid was not explained. I was looking for it. Please reply in comment both the function of potassium acetate and acetic acid. Thank you
amazing and very helpful
You guys are awesome