Size Exclusion Chromatography | Lab Procedure

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  • čas přidán 24. 10. 2018
  • #BaaYo
    Gel filtration chromatography (also known as size exclusion chromatography, molecular sieve chromatography, or gel permeation chromatography) is a chromatographic method in which molecules in solution are separated by their size, and in some cases molecular weight.
    In gel filtration chromatography, the stationary phase is comprised of porous beads packed into a column. The mobile phase is the running buffer or other solvent. Sample components partition between the stationary and mobile phases based on their size-based accessibility to the pores of the matrix beads.
    The smaller the size of the molecule or particle, the greater access it has to the pores of the matrix (or the accessible stationary phase), and the slower it moves through the column. Very large particles, which are completely excluded from the pores, elute first from the column at the void volume.
    The separated components are visualized as a plot of the volume of the mobile phase eluted through the column versus the detector signal. This plot, called a chromatogram, shows the location of the individual peaks and the quality of resolution of these peaks.The location of the peaks, their heights, and widths convey a great deal of information about the sample components as well as the column.
    In this video we have discussed complete laboratory procedure of size exclusion chromatography and also, how to calculate KD (distribution coefficient) of a particular component from chromatogram (Graph) and how KD valve can be use to determine the molecular weights of the sample components.
    Please watch this video for more details.
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Komentáře • 210

  • @BaaYo
    @BaaYo  Před 9 měsíci

    To access notes and practice questions, please join our Telegram channel t.me/baayo_official

  • @Scholars_Speaks
    @Scholars_Speaks Před 3 lety +15

    You deserve millions of likes.....best conceptual teacher ever. Much love and respect from Pakistan😍😍😍😍😍😍😍

  • @marva7991
    @marva7991 Před 2 lety +1

    Thank you so much such a brief yet descriptive video. Loved learning about it. I used to find this topic boring but not now :)

  • @cookiebank6909
    @cookiebank6909 Před rokem +1

    Mam u are really great ,i understand all chromatography techniques one day before exam . Amazing love from Pakistan today ❤❤❤

  • @razorr1920
    @razorr1920 Před 4 lety +12

    Just wanted to say thanks and congratulate you on the good work you have put through. I am a Flight engineer by profession, but love to learn. I found your presentation of the topic very elaborate and accurate. Will be following you...😊

    • @BaaYo
      @BaaYo  Před 4 lety +3

      Thanks a lot 😊, your feedback is really motivating 😊🙏🏻

  • @devaguptapuchayasri8842

    Ma'am thank you very much for the brief explanation... I'm now able to learn different Chromatographic techniques easily by watching your videos..

  • @sidraghu1c
    @sidraghu1c Před 2 lety +5

    Mam your way of teaching is marvelous.
    You are really helping alot to make our concept crisp clear
    Waiting for more videos
    Thanks alot mam

  • @ajimshaikh8779
    @ajimshaikh8779 Před 4 lety +1

    Thanks ma'am way of explaining is very good can easily understand...

  • @VijayPatel-cr8pq
    @VijayPatel-cr8pq Před 2 lety +2

    Very nice maam...🙏

  • @pragatimishra786
    @pragatimishra786 Před 2 lety

    Easily explained concept ...
    Thank you Ma'am.

  • @anchalkoundal745
    @anchalkoundal745 Před 5 lety +11

    Mam thank you so much for your all videos on types of chromatography. Because of these videos I am able to learn chromatography and their types very easily ...thank you for your help 😊😊 .

    • @BaaYo
      @BaaYo  Před 5 lety +3

      Thank You very much Anchal 😊😊, really like you comment.

  • @manjulasatishmanjulasatish85

    Thank you so much mam u r the saviour!! explanation is mind blowing ❤

  • @mdaabidhussain7039
    @mdaabidhussain7039 Před 4 lety

    Ma'am aapka smjhane ka tareeka bhut accha hai.. Thank you ma'am 😊♥️

  • @19deepikayadav14
    @19deepikayadav14 Před rokem

    Very nicely explained mam
    Tysm sab samjha.

  • @siraj2343
    @siraj2343 Před 4 lety +7

    we need a lecture on Sedimentation basic principles of centrifugation.. and maam ur videos are so good and helpful for us .

  • @priyalgupta9380
    @priyalgupta9380 Před 2 lety

    Thank you so much man

  • @hayaahmad8133
    @hayaahmad8133 Před rokem

    Whole topic was explained well!
    Love from PAKISTAN...
    KEEP IT UP 👍

  • @jaydeepdumadiya2464
    @jaydeepdumadiya2464 Před 3 lety

    Thank you ma'am ...
    GOD BLESS YOU

  • @muhammadumer4759
    @muhammadumer4759 Před 3 lety

    Mam ,i really appreciate u for sharing such valuable information .

  • @chinmayeethakur5223
    @chinmayeethakur5223 Před rokem

    So clear and concise ♥️

  • @shikamarunara2827
    @shikamarunara2827 Před rokem

    Wish my teachers could teach like u

  • @radheradhe1371
    @radheradhe1371 Před 2 lety +1

    Mam iam loving ❤️❤️ your teching way

  • @musicstudio554
    @musicstudio554 Před 3 lety

    I Learn main basic concept about technique in your lecture mam thank u so much mam for very informative easy lecture

  • @nuzhatkanoje3075
    @nuzhatkanoje3075 Před 2 lety

    It's very good video, help in my studies and my dought get cleared my watching these videos

  • @sktek370
    @sktek370 Před 4 lety

    Thanking you mam for these videos 🙏

  • @pritipandey6083
    @pritipandey6083 Před 3 měsíci

    Thank you so much ma'am ❤

  • @akhileshverma9978
    @akhileshverma9978 Před 3 lety

    Thank you Maam you made it soo easy 👍

  • @zainabqureshi9178
    @zainabqureshi9178 Před rokem

    You're Brilliant

  • @rohitdhiman1931
    @rohitdhiman1931 Před 2 lety

    Thank you so much for clear my concept

  • @DpkTamta157
    @DpkTamta157 Před 3 lety

    Thanku mam ap bahut achha explaine krte ho.

  • @YogendraYadav_16
    @YogendraYadav_16 Před 6 měsíci

    Thank You

  • @musicstudio554
    @musicstudio554 Před 3 lety

    Excellent

  • @nazianoreen9192
    @nazianoreen9192 Před rokem

    ❤❤❤❤outstanding teacher

  • @ajayprakash4307
    @ajayprakash4307 Před 2 lety

    Thankuuuú Ma'm your Explanation Technique is So Good and Incredible 👌👌😍😍

  • @AmandeepKaur-cy8ds
    @AmandeepKaur-cy8ds Před 3 lety

    It was well explained in a very simple manner 👍👍

  • @nasirchauhan3622
    @nasirchauhan3622 Před 3 lety

    You are a brilliant teacher Mam and cute also you are beautibrillinty teachers

  • @monica6899
    @monica6899 Před 2 lety +1

    Thank you soo much mam now I understand chromatography easily thank you once again for your efforts 🖤✨ Love from Nagpur . ❤️

  • @niharikakashyap5649
    @niharikakashyap5649 Před 2 lety

    Thank you so much mem this video has helped me a lot 🙏🙏🙏

  • @quickbiology5159
    @quickbiology5159 Před rokem

    Mam you are amazing ❤️

  • @shubhamgade3164
    @shubhamgade3164 Před rokem

    Mam your videos are very easy to understand And your explanation is very nice thank u mam

  • @anupriyamedhi2729
    @anupriyamedhi2729 Před 4 lety +4

    Thank you so much maam.😊

  • @saurabhshrivastva4641
    @saurabhshrivastva4641 Před 3 lety

    ma'am Bahut achha samjhati hain ap.........Dhanyavad👌👌

  • @liyaquatali755
    @liyaquatali755 Před 4 lety

    Thanks

  • @mohammadshadab5494
    @mohammadshadab5494 Před 4 lety

    thanks

  • @suraj.rathod4670
    @suraj.rathod4670 Před 2 lety

    Good explanation

  • @sunilmaahi
    @sunilmaahi Před 5 lety +1

    every video is superb with quality... thnx

    • @BaaYo
      @BaaYo  Před 5 lety

      Thank You Sunil 🙏🏻😊

  • @diadaksh7149
    @diadaksh7149 Před rokem

    Thanku very much mam

  • @pradeepkumarsingh742
    @pradeepkumarsingh742 Před 4 lety +1

    very nice mam

  • @bingeb3543
    @bingeb3543 Před 4 lety

    U r superbly good

  • @neerajacw
    @neerajacw Před 5 lety

    Thanks for sharing

  • @chiragpatel9248
    @chiragpatel9248 Před 2 lety

    Superb..👌👌👌👌

  • @aysha840
    @aysha840 Před 4 lety +1

    Assalam o alikum Mam very impressed by your teaching method. 💓🙋

  • @ayeshajabeen2654
    @ayeshajabeen2654 Před 3 lety

    Superb explanation mam.

  • @madhumitanishad9462
    @madhumitanishad9462 Před 4 lety

    Your voice and your teaching techniques is very good maim👌👌👏

  • @laraibzia8905
    @laraibzia8905 Před 2 lety

    you are best

  • @aabanalawade1
    @aabanalawade1 Před 3 lety

    Thank u Mam.😊

  • @shaikayeshasiddiqi3895

    Tqs to u mam to clear al doubts about these topics
    Happy teachers day💐mam

  • @shanu9094
    @shanu9094 Před 5 lety +5

    Fantastic mam
    I saw your all vedios based upon chromatography and I just loved the way u described every single point of topic very clearly 😊😊😊😊

    • @BaaYo
      @BaaYo  Před 5 lety

      Thank You Shanu 😊, I am glad that my videos could help you with these topics

    • @shanu9094
      @shanu9094 Před 5 lety +1

      @@BaaYo mam I don't like those vedio which based on electrophoresis on you tube plzzz upload your vedios on the topic electrophoresis

    • @BaaYo
      @BaaYo  Před 5 lety

      its live now : czcams.com/video/citMv0hvfyc/video.html hope it will met with your expectation

  • @waqarch8333
    @waqarch8333 Před 3 lety

    Well explained

  • @priyatabassum1190
    @priyatabassum1190 Před 5 lety

    really helpful

  • @sehrishkanwal2070
    @sehrishkanwal2070 Před 3 lety

    thank u so much mam

  • @dipesh7921
    @dipesh7921 Před 5 lety

    mam apke to diwane ho gye

  • @hazratullahkhan4661
    @hazratullahkhan4661 Před rokem

    Beautiful lecture with beautiful face ❤❤

  • @darshakeditz9591
    @darshakeditz9591 Před 2 lety

    Mind blowing di👌👌

  • @ramchandrasuthar2848
    @ramchandrasuthar2848 Před 5 lety +1

    Great analysis

    • @BaaYo
      @BaaYo  Před 5 lety

      Thanks Ramchandra 😊

  • @navnathdhage632
    @navnathdhage632 Před 5 lety +2

    mam ur teaching is very clear and very nice speaking and also they are very helpfull

    • @BaaYo
      @BaaYo  Před 5 lety +1

      Thank You Navath 🙏🏻😊

  • @yogeshanjansinger2872
    @yogeshanjansinger2872 Před 4 lety

    Very very Thanku mam 😊😊

  • @pushprajpatel1217
    @pushprajpatel1217 Před 5 lety

    Great mam

  • @loardsofcooking4537
    @loardsofcooking4537 Před 5 lety

    Bht acha

  • @narendraverma1017
    @narendraverma1017 Před 2 lety

    Awesome explanation and nice presentation madam

  • @hks9899
    @hks9899 Před 3 lety

    Thanks mam🙏

  • @suhalah
    @suhalah Před rokem

    Dynamic light spectroscopy would be very helpful

  • @shivaramsmjadhav3298
    @shivaramsmjadhav3298 Před 4 lety

    U have not discussed about the type of detector is used in gel filtration/permeability chromatography or size exclusion C.

  • @kainatdarwaish3979
    @kainatdarwaish3979 Před 5 lety

    Mam plz give me answer of my this question not realated to chromatography ...
    In what way is electrodless discharge lamp better than hollow cathode lamp

  • @saritasharma6557
    @saritasharma6557 Před rokem

    Nice

  • @priyankavhatkar8070
    @priyankavhatkar8070 Před 2 lety

    The Way u teach is too good..Perfection💯. Thank you.

    • @BaaYo
      @BaaYo  Před 2 lety +1

      It's my pleasure

    • @ajayprakash4307
      @ajayprakash4307 Před 2 lety

      Hii
      Priyanka where r u from
      You have and notes of MSC CHEMISTRY 4th Sem?

  • @razorr1920
    @razorr1920 Před 4 lety

    Just a small question. How does the detector detect the elute sample?

  • @ImranAhmad-df3mi
    @ImranAhmad-df3mi Před 5 lety

    Nyc explanation mam

  • @Together_Stronger
    @Together_Stronger Před 5 lety

    Thank you mam 😊

  • @abidhussain2484
    @abidhussain2484 Před 5 lety +1

    mam i like your every lecture... thanks for teach us

    • @BaaYo
      @BaaYo  Před 5 lety +1

      Thanks abid..Please share this video with your friends also.

  • @sahirahmad7091
    @sahirahmad7091 Před 3 lety

    Mam i like ur lectures so much.ur way of teaching is very interesting.
    Mam please make some videos on basics of centrifugation.

  • @learnwitheaglestar
    @learnwitheaglestar Před rokem

    Very informative and easy understanding

  • @justinfaiz1186
    @justinfaiz1186 Před 4 lety

    Aslamu alaikum
    Mam your way of teaching is outstanding. MashaAllah simpler and easier but please include difinitions in your lectures for notes .it will be your kindness .hope you act on it thnx

  • @mridul7820
    @mridul7820 Před 5 lety +1

    Thank you Aarti Mam👍👍👍👍👍

    • @BaaYo
      @BaaYo  Před 5 lety

      Thanks Mridul 😊

  • @faizakahloon7961
    @faizakahloon7961 Před 5 lety

    v good

  • @SafdarHussain-cn3fr
    @SafdarHussain-cn3fr Před 3 lety

    thnks alot mam

  • @POOJACHAUHAN-le9sg
    @POOJACHAUHAN-le9sg Před 2 lety +1

    Hello mam your video clear my concept and very useful for pharmacy profession .I request you to please make details video on hplc
    And handling of hplc and it's software please I request depth of my heart

  • @sammrayounas2681
    @sammrayounas2681 Před 3 lety

    After the separation of large size molecules which component separate first intermediate or small size component of the sample...???
    If intermediate eluted after large size molecules then why intermediate eluted first not small size reason???

  • @SURAJSINGH-np9nm
    @SURAJSINGH-np9nm Před 5 lety

    Thank you mam

  • @catchascatchcan8994
    @catchascatchcan8994 Před 4 lety

    Some says gel filtration is a type of size exclusion chromatography and some says it is another name of it. Which one is correct?

  • @mrniravpatel9591
    @mrniravpatel9591 Před 5 lety +31

    I learn main basic principles about chromatography in your video
    I am not learn this kind of basic thing in my 2 years carrier in industries

    • @BaaYo
      @BaaYo  Před 5 lety +1

      🙏🏻🙏🏻 thanks Nirav.

    • @Ankitsingh-qf6el
      @Ankitsingh-qf6el Před 2 lety +2

      Bhai kaun si industry m kaam kr rahe the😂😂

    • @jhalendraverma7415
      @jhalendraverma7415 Před 2 lety +1

      @@Ankitsingh-qf6el ab to 5 saal ho gya Hoga😂

  • @AdityaBalchanne
    @AdityaBalchanne Před 7 měsíci

    Mam how to check methan gas by simple chromatography, becoz hamare clg me gas chromatography nhi hai lekin muje bio gas me se methan gas ko analyse krna hai to kaise kru

  • @NiranjanKathavate
    @NiranjanKathavate Před 10 měsíci

    thankyou maam i am loving chromatography

    • @BaaYo
      @BaaYo  Před 10 měsíci +1

      Thank you

  • @sadiquenirban7299
    @sadiquenirban7299 Před 5 lety +1

    Osm mam😍😍

  • @syed-wd8ib
    @syed-wd8ib Před 3 lety

    how can we know about the total volume which is use for elution?
    Is it when we get zero concentration at the last? Kindly reply

  • @rajdeepnegi6526
    @rajdeepnegi6526 Před 5 lety

    Mam what is void volume and dead volume in chromatography pls tell about..

  • @arpitgoel2481
    @arpitgoel2481 Před 5 lety

    What is basic principle of size exclusion chromatography??
    Please explain!

  • @premaadhikarii
    @premaadhikarii Před 2 lety

    Mam gel chromatography be esko he khte hai kya

  • @bhatusuryawanshi1089
    @bhatusuryawanshi1089 Před 3 lety

    Hi mam, your video on size exclusive chromatography is very good. I learn about the importance principle of GPC. Mam can you plz explian about types of detector used in GPC and their working .

  • @start5256
    @start5256 Před 5 lety

    good explanation mam..can u explain with examples of elution profiles of different chromatograms?