Microbiome Informatics: OTUs vs. ASVs | Zymo Research

In this video, we review the basic concepts of OTU and ASV-based methods of targeted microbiome sequencing resolution. Topics will include a basic introduction to targeted microbiome sequencing, the methods and logic behind both OTU and ASV generation, and the advantages and disadvantages of each method for 16S and other targeted sequencing studies.
Read more about OTUs vs ASVs, including the advantages and disadvantages of each when dealing with difficult sample types here:
www.zymoresearch.com/blogs/bl...
To find out more about how we can assist in your microbiome studies, please check out: www.zymoresearch.com/pages/mi... and www.zymoresearch.com/pages/zy...
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[@zunjuu]

Thanks, thats a brilliant video. The last point made me smile. How many times have we all read "OTU grouping" autocorrected to "OUT grouping".

[@hpremathilake]

Like the concluding remarks :D

[@ktburger659]

Great video, thanks so much

[@soniavargas7947]

this was an excellent video, thank you so much! The autocorrect made me laugh, as this has victimized me in the past.

[@adeelmanzoor9801]

I think the instructor should slow down as speedy talk make understanding such technical topics more difficult.

[@loxix100loxix100]

how do we know if the sequence is or isn’t conserved?

[@kiawendy745]

fantastic video, thanksss

[@ZymoResearch]

Glad you liked it!

[@Crazy09starkillor]

If i were to use ASV, do I still need to run DADA2 or amplicon denoise before hand?

[@couchpatata6835]

When you run your sequences using DADA2 (I used the DADA2 plugin in QIIME2), your output are ASVs.

[@pankajchand6761]

Why does the speaker need to speak so fast when explaining a technical topic?

[@edodanilyan]

Well, you can slow it down, there's a feature to do that in CZcams