lecture 25 : Protein Purification by Size Exclusion Chromatography (SEC)

FPLC, size exclusion chromatography, peristaltic pump, gravity separation, UV detector, SDS PAGE gel

[@amiraakilah8950]

i ve been for 3 months in laboratory and i could not ever understand it until you explained it that was .. many many thanks , keep on

[@palvoliuto6059]

that was a fabulous video!!!!! clear, precise and detailed. thank you so much!!!!!!

[@ravikiran780]

Thanks a ton for making this video and a great job at explaining the process thoroughly!

[@khalilelbadri5371]

Thank you for such amazing lecture.

[@dhruvkumarsoni5344]

Very nicely explained. Thanks! Best wishes.

[@devikasharma2406]

I loved this video....I have an interview at my workplace in Sydney please pray for me.

[@JeanStorM]

Thanks so much! helpful and detailed video for beginner.

[@prudencephodisomashile4909]

This is awesome, thank you so much for such detailed explanation.

[@adronung1892]

When you are talking about the olden days that is exactly what I do now. However, I syphon buffer onto the column so do I don't have to manually add buffer to the column, and I automatically collect fractions with a spin collector. I used Bradford assay to find the bluest fractions where the protein is present, and I check the A280 of those fractions.

[@LucasRegmi]

Great

[@SteSews]

Hello, I do have a probleme with my Akta system, the UV sensor doesn't work, I can't obtain a graph with UV variation. I do see the UV variation in the little screen wich is on the machine, but there is nothing on the graph so it's very hard to know where my samples are...Does anyone have an idea on how I can fix this please let me know ! thanks

[@shawnaguheen4270]

Thanks so much!!

[@diponsaha5357]

Thank you Dada

[@tamerwali4598]

Great, which you prefer this sys or NGC and why?

[@syed-wd8ib]

How machine knows about the total volume used in elution?

[@loveforbioinfo]

Nice video but too many repetitions which increases video time