Lecture 53 : Western Blotting Technique

Antibody interaction, SDS PAGE, Immuno blotting technique, PVDF membrane, western blotting, nitrocellulose, transfer buffer, bovine serum albumin, TBST buffer, primary antibody, secondary antibody, HRP enzyme

[@mamtadiwan1118]

Beautifully explained!👏

[@nikitaraorane1867]

Thoroughly explained with demonstration,
We expected the results also thank you

[@SasiSasi-cc5ys]

very informative and excellent explanation.pls demonstrate the actual results

[@joshuastephenkingsly]

Very nice explanation. Was expecting to see the result though😅.

[@rameshram-hm3jh]

Great explained

[@yoyaoo8267]

Excellent thanks for sharing

[@splendidscience7669]

so clear cut explanation. plj also make video on PCR techniq.

[@dhrumilkapatel9235]

Simply Impeccable

[@NARANGI113]

Very informative 👍

[@khadijabegum4333]

Very nice

[@mdzulfekarali9133]

extra ordinary thank u

[@suganyasivakumar6972]

Kindly let me know the significance of tris and glycine in transfer buffers .. as I know the glycine is a zwitter ion and at 8.3 ph , it will be negatively charged and( it helps in separating gel in sds to release the sds bound protein , which was previously stacked between cl and glycine at 6.8 ph . ) what significance it has in transfer buffer ..

[@Sambedana221]

To remove SDS from SDS bound protein

[@mridulasharma6171]

What is the cost of this biorad fast transfer turbo

[@bharathreddy9337]

Fantastic

[@kirtipatel8848]

thank you mam

[@rameshram-hm3jh]

Great explany

[@nitinsharma4307]

Nice👍

[@hilloldas78]

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