Chromatography 101: An Introduction to Size Exclusion Chromatography
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- čas přidán 6. 07. 2024
- For more information, visit www.bio-rad.com/yt/31/ngc.
Jim Maher presents an introduction to size exclusion chromatography using Bio-Rad's chromatography instruments, columns, and media to demonstrate the technique. Jim covers the basic principles of size exclusion chromatography and touches on applications for the separation of proteins and other biomolecules using a variety of size exclusion media.
Bio-Rad's Successful Chromatography Webinar series provides a great introduction to the different chromatography methods available for research applications.
Most medium pressure chromatography columns work with Bio-Rad's NGC preparative chromatography system. The NGC is designed to rapidly automate the purification of biomolecules. The flexible, modular, and economical design makes the NGC systems the instruments of choice for method development and scale-up.
NGC systems are available in six preplumbed, factory-tested configurations with two different flow rates. Each preconfigured system can be further customized and upgraded by adding valves, detectors, or pumps to meet specific application needs. Any system can be configured for operation at either high or low flow rate by simply changing the pump head on the system pump module. With the NGC system, a single hardware platform can be modified as needed to suit changing applications and scales.
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this would be a lot better if you were not using a toaster for a mic, still good info though so thanks.
A special thanks from Brazil! Obrigado!
Thanx for the upload
thank you very much
Good video, but the sound quality is so low that it becomes difficult to hear what is being said.
Does the factors affecting resolution for group separation affect mass spectrometer resolution. For example improved chromatography resolution leads to improved Mass spectrometer resolution?
***** And that my point BIORAD, I want to couple a HPLC SEC with ESI MASS spectrometer. I need to be able to desalt proteins online - so from SEC straight into the Mass spec. I'm basically trying to make them both compatible. maybe im asking the wrong question. i wanted to know how i would do this.
***** Hey BIODRAD. Thanks for you help. My project is well underway and I have managed to couple my system yayaya. I was so confused. Acutally the SEC column is used to improve S/N in MS spectra. Lets hope an A+ comes my way for my masters. ;)
WHY IS IT NECESSARY TO EQUILIBRATE THE COLUMN PRIOR TO SEPARATION
You want to make sure you have the proper buffer before you load your sample, otherwise your protein may not bind when you need it to or may bind when you don't want it to.