Microbiology: the Catalase test

Using the catalase test to see if the enzyme is present as a virulence factor in a bacteria.

[@paulnagami501]

Thanks! I'm showing this to students as a medically-relevant example of an enzyme assay!

[@MedicalLabLadyGill]

Thank you for your support!

[@8v8i]

this is how microbiology should be taught.

[@MuhammadHaris-rj8ku]

Thnkx , will definitly help in tommorow practical exam, 😊

[@laurasookhai7802]

Thanks for breaking this down!!

[@MedicalLabLadyGill]

You're welcome!

[@karimmohaseb]

Hello, How to prepare the hydrogen peroxide for catalase test? I mean what percent is the Hydrogen Peroxide?
Thanks

[@MedicalLabLadyGill]

I am using an over the counter standard 3% Hydrogen peroxide here.

[@ivanbombana7282]

Catalase is just one enzyme or a group of enzymes? Thanks 😁

[@MedicalLabLadyGill]

Catalase is one enzyme produce by cells that are exposed to oxygen to combat against oxidative stress on the cell.

[@mustafapisirici9969]

thank you. i would like to ask a question about tiny bubbles. if any tiny bubble appears on the slide, is it catalase positive or catalase negative?

[@MedicalLabLadyGill]

Bubbles are positive. They bubbles may become larger if you move the bacteria around after the H202 is introduced.

[@lucilaalvarez4095]

Thank you 🙏

[@MedicalLabLadyGill]

Thank you for your support! Blessings!

[@user-nr2oy4hh7w]

Good

[@user-mg8tu6jb8b]

It's very excellent way to explain thank you ❤️

[@MedicalLabLadyGill]

Glad to help! Thank you for your support!

[@gabrielb.desouza8632]

If the bacteria has catalase, does it mean it is aerobic ou anaerobic?

[@MedicalLabLadyGill]

Great question! It could be either. Facultatitive anaerobes and aerobic bacteria can be catalase positive.

[@vjiglesias5815]

So in other words, the strep gets destroyed by the hydrogen peroxide as where catalase in staph breaks down the hydrogen peroxide??

[@MedicalLabLadyGill]

That is correct.

[@rianesyafierah]

does c.diphteria also show the exact same reaction as s.aureus too?

[@rishikasharma9326]

Thanks 😊

[@MedicalLabLadyGill]

Thank you for your support!

[@maram6382]

Thanks 💜

[@awosusitemitope2232]

Thanks 🙏

[@user-fz2gg6po6n]

Thank you

[@MedicalLabLadyGill]

Thank you for your support!

[@saikatpatra4614]

Thank you ❤️❤️

[@MedicalLabLadyGill]

Absolutely!

[@adilaitsalm4354]

thanks

[@MedicalLabLadyGill]

Thank you for your support!

[@skasmow]

I am 29 and have white hairs from almost 1,5 year now. If i will do this test and i have catalase that means my white hairs are not due to genetics but other factors?

[@MedicalLabLadyGill]

I am not sure where that conclusion originated but it is innacurate.

[@skasmow]

​@@MedicalLabLadyGill in some people catalase stop working due to genetics..thats why we are getting white hairs

[@MedicalLabLadyGill]

This is a test for bacterial cell analysis. I have not heard of the human catalase test. If that is something you're looking for, this is not that test.

[@skasmow]

@@MedicalLabLadyGill οk thanks! yes their is a test for catalase test for human

[@shadymedic]

Damn, this makes me thirsty for some soda...

[@Isla-bn3wk]

thank you so much I finally understand

[@MedicalLabLadyGill]

So glad! Thank you for your support!

[@elbertek5836]

ÇOK İİ

[@meganmaroky455]

Thank you! This is real helpful :) For God so loved the world He gave His one and only Son so that whoever believes in Him will not perish but have eternal life- John 3:16