How to Tissue Culture a Pothos from Start to Finish (Epipremnum Pinnatum)
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- čas přidán 16. 03. 2023
- Although I used TDZ (Thidiazuron) in this video, I no longer use that plant growth regulator because it can have adverse effects on human health. Always be really careful when handling plant growth regulators.
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(DISCLAIMER: PPM is a biocide, so it can harm anything biological - including you and the environment. Although it may help reduce contamination, use with caution. Always dispose of expired chemicals correctly at a hazardous waste drop off and never pour them down the drain, as these chemicals can pose a major threat to our water systems and aquatic organisms.)
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Fantastic. I love your videos. Keep them coming
Thai constellation video coming right up!
This is so cool. I just assumed TC was professional lab level expensive and equally complicated. It still looks rather complicated. 😋 Good luck to you, and keep up the vids!
Thank you!
Hey what do you use as an alternative for TDZ now?
Very similar to mycology! I'm confused though, because i propogate pothos and most plants in soil or pumice with a really high success rate. For pothos its basically 100%
What protocol are you using? I’m about to attempt to tissue culture a golden pothos. I’d love to read it if you have a link.
I went back in my notes and I think I was loosely following this one: link.springer.com/article/10.1007/s11240-013-0319-x
What was the end result for this cebu blue? Did the protocol work?
Yea it worked, I got a bunch of shoots to form and some baby plants. I discarded the cultures eventually because I don't really want a bunch of baby cebu blue plants.
What’s the difference between tdz and bap? I see ppl using bap in media , you don’t use it for example philodendron ?
BAP and TDZ are both cytokinins, so both promote shoot formation. The protocol I was following for this experiment called for TDZ, but I have also seen BA used for pothos plants as well. Different cytokinins work better for different plants. TDZ is common for philodendrons as well.
Hello!! Your video is fantastic! I think I'll try to multiply photos in my house! Your videos help me a lot.
I bought BAP and NAA but I don't know if with your recipe I could substitute the TDZ for BAP, have you tried it? Do you think it could work? I hope I can tell you about it in the future! =) It confuses me how different PGRs are used for each plant, it's a lot of investment of money! At the moment, I only use BAP, although I'm starting... do you recommend any other cytokinin to be able to carry out the Araceae protocols?
I would love for you to make a video about philodendron, it's my favorite plant and I haven't managed to reproduce it in vitro yet! 🤣
You're great. Your videos are too! Thank you!!!
My two most recent videos are about philodendrons! 1.0mg/L of BAP works for philodendrons, no real reason to buy TDZ as well unless you wanted to compare the results! TDZ is great for creating a lot of callus tissue, but that's not necessarily what you always want :)
What kind of alcohol do you use in your alcohol burner?
90% isopropryl
I'm a newbie in tissue culture. I bought pure IBA powder and dissolved it in water using ethanol and then added it to my medium and autoclaved it but no matter how much I tried, I couldn't see any callus formation around the explants(leaves). My IBA concentration is 4mg/L. I read on Wikipedia that IBA is heat sensitive and will get evaporated by heat. Maybe autoclaving destroyed my IBA? Can u help me figure out what's the problem?
Would you mind sharing what type of plant you are tissue culturing? 4mg/L is a high concentration of any PGR. Are you adjusting the pH of the media before autoclaving? I've found some plants can be really sensitive to pH levels (both in tissue culture and mature adult plants)!
@@plantsinjars I'm tissue culturing persian walnut (juglans regia) and almond using their mature leaves as my explants. Unfortunately I don't have a PH meter :( my TC medium consists of 4.4gr/L MS (murashige and skoog)
30gr/L sugar
8gr/L agar
4mg/L IBA
2gr/L activated charcoal
I didn't use any kind of antibiotics or PPM
Any help would be appreciated :)
@@plantsinjars is having a ph meter essential for tissue culturing? If yes, can u make a video about how to adjust the ph of a TC medium and what type of ph meter is more suitable for tissue culturing at home? It would be enormously helpful for newbies like me :)
@@mahyarshokraeian I think the pH is definitely the issue then! Measuring the pH is essential. If you don't have a pH meter, you should see if you can get pH strips. They work just as well and you don't need to calibrate them. To increase the pH of the media, I add a few drops of hydroponic pH up at a time. If you live somewhere where you can't get hydroponic supplies, then you can also you add diluted HCl or NaOH solution to lower/raise the pH. I haven't tried persian walnut plants but most plants like a pH around 5.8 in vitro.
@@plantsinjars thanks for helping me :) 💗✨ I'll try adjusting my ph to 5.8 next time and see what happens. Ohh.. I had another question too... It's about PGRs such as IBA. Doesn't autoclaving destroy these PGRs? Aren't these PGRs heat-sensitive?
Think you meant to say Sodium Hypoclorite instead of Hydrocloride. Great videos though!