Microscopy: Setting Up Koehler Illumination (Ron Vale)

Learn more: www.ibiology.org/talks/set-up...
Koehler illumination is the preferred method for obtaining even illumination of the specimen and is a critical alignment procedure in microscopy. This lab goes through a step-by-step procedure of aligning the lamp and condenser to achieve Koehler illumination.

[@vajihehjafari9682]

thanx,it helped me a lot

[@saliyagurusinghe7148]

Thank you!!! Life saver

[@eleonoralugara6753]

Hello, Thank you for these videos, they are really well made!
I have a question. In my setup I have two objective (10x and 60x). I use both of them everyday for two different type of experiments (Local field potential and patch clamp). Is it possible to have optimal Koehler illumination for both of them at the same time or is it better that I adjust the K.I. at 60x (when the shape and resolution of my cells are critical)?
Thank you in advance!

[@victortn99]

As far as I know, Koehler alligment cant be performed at higher than 10x magnifications

[@themonk3460]

It could be great to show light path animation also to show how things are changing, same time representing the different part of microscope so one could see how light path changing when we are changing something in the microscope that way it could be more comprehensive learning rather just memorizing how things would be changing in the light path specially for someone who don’t remember or have in depth knowledge of the instrument but could memorize by these extra animations in half window that way learning could be more in depth.

[@noorchawla3968]

really helpful..:)

[@lotharmayring6063]

Wieso muss denn beim Koehlern der Kondensor zentriert werden. Seine optisch Achse sollte doch in jedem Fall
mit der optischen Achse des Objektives uebereinstimmen und nicht mehr veraendert werden. Wohl aber
sollte die Leuchtfeldblende zentrierbar sein.
Das bedingt auch immer eine zentrierbare Punktlichtquelle.

[@Moveon10001]

Everything is upside down! I'm used to a regular PLM.

[@dionisiochavez9570]

The explanation was good. But it would have been better if the procedure was demonstrated on a 'basic' microscope.

[@donsimons9810]

you all have no clue who just gave you a lesson with this microscope. Oblivious, whining, excuse-making children and the problem with science! Besides, why wouldn't you want to see how it's done with a microscope you might actually use in modern times?