Introduction to SMRT Sequencing
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- čas přidán 4. 12. 2011
- A brief animated introduction to Pacific Biosciences' Single Molecule, Real-Time (SMRT) Sequencing, including the SMRT Cell and zero mode waveguide (ZMW).
Note: NO AUDIO in this animation. Explanatory text is included.
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Sound quality is GREAT!
😂😂😂😂
Imagine you are an international student paying 5,000 dollars for this course to learn in university and they are sent to off to watch an advertisement video from a tech company, a silent comedy film, no less.
while the fees you mention are quite high for where I'm from, I see their point in sensing you to this video. it's a fast way to grasp a concept you will only use as a tool later on and don't need to understand from the ground up.
..unless u study engineering and are supposed to build such machine in which case these 2 minutes wouldn't cut it xD
but for molecBio it is enough
ps: i was sent here too
10^-21 liters is a single zeptoliter, not 20 zeptoliters.
Jonger Chua: the way I understand it is that the size of the chamber is such that the illuminating light pulse only passes far enough into the wave guide to excite the markers which then emit their frequency out the guide at the top to the detector. If the illuminating pulse were to continue through the waveguide the detector would essentially be "blinded". This is how they achieve the high signal to noise needed for accurate recording.
clever boy
the explanation of the wavelight at the beginning is just to tell that the light penetrates very shortly in the guide, just enough to eccitate the released fluorophore in order to be detected, since it needs to be eccitated by uv light to be detected, otherwise you could see all possible background light given by sopntaneous reaction or whatever
Nice video. Clear explanations and animations.
Could you help me explain what happen at the other end? Can you somehow fix two polymerase at the bottom? Do both forward and reverse synthesis happen at the same time? Thanks.
Great Video. Im studying biotechnology and this helped me a lot in genomes.
sameeeeee
Amazing.
Great Video. Im studying biotechnology and this helped me a lot in genomes.
I don't really understand 0:23. mind explaining?
any one understand how excaly hesy choose or isolate a single dna fragment or template of desire ?
and how will they put a genome for example or the excome by which each fragment will be in 1 zmw
No audio?
i aslo can not hear the voice