thank you so much. Is there standard to use silica gel in column, I mean if my sample a little bit like (0.01 gram), how much of silica gel use here? in contrast, if I have a lot of samples such as (1 gram or more), here, how much of silica gel have to use? I mean is there ratio from silica gel to sample to use in column, please tell me
thank you. If I used wet packing of silica gel to column (slurry), here, can I use any solvent for silica gel until reached slurried, or same mobile phase or must use only hexane.
Firstly, excellent demonstration. Can I please ask, why did you add a second layer of sand above the silica layer? When I perform chromatography, I never add a second layer. I just swirl my sample around the inner surface. Will it be advantageous to add a second layer of sand? Thank you
@@channychan3558 Wouldnt that be when you load sample first and then add the sand on top? I have seen a vid of someone demonstrating that. I dont know whether to load the sample before the sand or after the sand. Also should we load the sample through pipette by making a ring via pippetting along and around the glass of the column or dropping it straight down the center to form the band. I ask since I've seen it done here on CZcams both ways. Just would like to know which is the common or preferred way.
If I have solid sample containing components, can I use any solvent to dissolve it? Or I have to use only the same mobile phase as solvent to dissolved sample? Another case, if must use same mobile phase, but my sample can not dissolve, here, please I need solution to this problem. can I use any solvent? thank you alot
A year late... But You need to figure it out by trial and error, through literation, or educated guess. Your goal is to get your material dissolved but separated during the column/elution. If you use the extraction solvent, most of the time you will get terrible separation. To figure out what eluent combination is best, try to do Thin Layer Chromatography tests in a series of polarity (example: from 10:1 hex:EA to 1:2 ratio) and see the separations. Use the solvent with the best separation and Rf of your target compounds
Hi, I have a question. I want to Pack column with silica. Which solvent is better for packing of column with silica. Is water is ok? because in mobile phase i will use ethyl acetate and methanol.
True, and I'm not advocating for gloves in 100% of situations. Here though, she is being an example for students to follow. As a TA, I can tell you that students need reminding to wear gloves
Because it's FUCKING USELESS! Since the moment when I started the video I knew that there would have been some lab safety freak commenting about the gloves.
No reason to wear gloves for every single thing we do in the lab. If I am mixing up sugar solutions, I am not wearing gloves. For most things gloves are not necessary. Use your brain, do a little research before working with a chemical. Don't just automatically put ppe on if there isn't a a good reason to.
Have you guys ever worked with methylene chloride? It can do some damage to skin, and although you can wash it off and be fine, it is very uncomfortable for even brief periods of time.
Camera person didn't know what meniscus was 🤣
These are great videos. Very useful. Thanks!
Thank you for your lovely presentation.
thank you so much. Is there standard to use silica gel in column, I mean if my sample a little bit like (0.01 gram), how much of silica gel use here?
in contrast, if I have a lot of samples such as (1 gram or more), here, how much of silica gel have to use?
I mean is there ratio from silica gel to sample to use in column, please tell me
thank you. If I used wet packing of silica gel to column (slurry),
here, can I use any solvent for silica gel until reached slurried, or same mobile phase or must use only hexane.
And i use the buffer solution to seperate my desired protein insted of solvents..??
Firstly, excellent demonstration. Can I please ask, why did you add a second layer of sand above the silica layer? When I perform chromatography, I never add a second layer. I just swirl my sample around the inner surface. Will it be advantageous to add a second layer of sand?
Thank you
That way you avoid accidentally disturbing the band of sample when you add the solvents to the column to actually start the separation.
@@channychan3558
Wouldnt that be when you load sample first and then add the sand on top? I have seen a vid of someone demonstrating that. I dont know whether to load the sample before the sand or after the sand. Also should we load the sample through pipette by making a ring via pippetting along and around the glass of the column or dropping it straight down the center to form the band. I ask since I've seen it done here on CZcams both ways. Just would like to know which is the common or preferred way.
How to separate the mixture of sample and solvent in reservoir
If I have solid sample containing components,
can I use any solvent to dissolve it?
Or I have to use only the same mobile phase as solvent to dissolved sample?
Another case, if must use same mobile phase, but my sample can not dissolve, here, please I need solution to this problem. can I use any solvent? thank you alot
I don't know if it's the music, but that was INTENSE!
imagine hearing it on 1.5 speed
Why does the operator show the stopcock and not the meniscus?!
What solvent do I use for the mobile phase? Is it going to be thesame solvent I used in extracting my plant material?
A year late... But You need to figure it out by trial and error, through literation, or educated guess. Your goal is to get your material dissolved but separated during the column/elution.
If you use the extraction solvent, most of the time you will get terrible separation. To figure out what eluent combination is best, try to do Thin Layer Chromatography tests in a series of polarity (example: from 10:1 hex:EA to 1:2 ratio) and see the separations. Use the solvent with the best separation and Rf of your target compounds
Hi how to make slurry of sample for loading a column
Hi, I have a question. I want to Pack column with silica. Which solvent is better for packing of column with silica. Is water is ok? because in mobile phase i will use ethyl acetate and methanol.
Always use the solvent you intend to elute with.
Water? Not unless you are eluting with water..
Thank you so much!But the camera wasn't doing a good job on it
Mam how can we separate very close spots?? I'm facing problem during the separation
Try to read about IATROSCAN DEVICE MK6 HAVE A LOOK AND ENJOY IT IS automated machine
Wow, would be interesting how is now the lab. Without hunger games effects...
Is silica gel and silica powder are same..?
Yea
Why music with study
Gloves?!?!
What is the sample??
@@dumbeau hi
Why isn't she wearing gloves?
TauridBorn 99%of the time, gloves aren’t really necessary in school labs. Usually not dealing with anything too hazardous.
True, and I'm not advocating for gloves in 100% of situations. Here though, she is being an example for students to follow. As a TA, I can tell you that students need reminding to wear gloves
Because it's FUCKING USELESS!
Since the moment when I started the video I knew that there would have been some lab safety freak commenting about the gloves.
No reason to wear gloves for every single thing we do in the lab. If I am mixing up sugar solutions, I am not wearing gloves. For most things gloves are not necessary. Use your brain, do a little research before working with a chemical. Don't just automatically put ppe on if there isn't a a good reason to.
Have you guys ever worked with methylene chloride? It can do some damage to skin, and although you can wash it off and be fine, it is very uncomfortable for even brief periods of time.
sfsu brought me here
where ıs your gloves? It is not safe sorry .
no gloves…?
Please make can you explain in hindi
Cmleddme lmanedre
Stopcock
Wear gloves when doing chemistry please!