Column Chromatography | MIT Digital Lab Techniques Manual

Column Chromatography
It takes considerable practice to master the art of "running a column". This video will get you started, with tips on picking appropriate conditions, packing and running a column, monitoring separation, and even making a micro-column from a pipet.
Created by Dr. Sarah Tabacco and Aaeyesha Siddiqui
View the complete resource at: ocw.mit.edu/OcwWeb/resources/R...
License: Creative Commons BY-NC-SA
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The experiments described in these materials are potentially hazardous and require a high level of safety training, special facilities and equipment, and supervision by appropriate individuals. You bear the sole responsibility, liability, and risk for the implementation of such safety procedures and measures. MIT shall have no responsibility, liability, or risk for the content or implementation of any of the material presented. Legal Notice - ocw.mit.edu/OcwWeb/web/terms/t...

[@shahub5]

this video quality just blows my mind

[@XiangxiMeng]

This one is canonical, and it is the first ever Flash Chromat video I have ever watched, and I review it every time I want to synthesis something.

[@jackie_126]

It's 2020 now, and this video is superb! Now I can make my own silica column.

[@ToanLe-xc2vn]

greatest video about column chromatography I've ever watched.

[@shaminisomasundaram]

it is very detailed
it is awesome
the video quality is just awesome

[@MsSydneyd]

Thanks so much! OChem is kicking my ass...

[@user-yp5my4uu6s]

I love the introduction music.

[@rahmadinta3315]

Thank you ❤️

[@karlbergen6826]

These instructions are for organic chemicals but this can also be done on inorganic chemicals but your solvents will be much more polar, probably water or water with a little acid or base.
Some elements have properties so similar they are had to separate, a classic example being lanthanides. In fact lanthanides sold commercially are often mixtures because of practicality.
Fractional crystallization of bromates was one of the methods used historically to separate lanthanides. What was thought to be one element resolved into two or more. Practical use was almost entirety with mixtures.
Then it was discovered that they could be separated with column chromatography. This was eventually done on a large scale.

[@salehchem4376]

Thank you so much. Is there standard to use silica gel in column, I mean if my sample a little bit like (0.01 gram), how much of silica gel use here?
in contrast, if I have a lot of samples such as (1 gram or more), here, how much of silica gel have to use?
I mean is there ratio from silica gel to sample to use in column, please tell me. thank u in advance

[@salehchem4376]

happy new year Dr. please I need your help .If I used wet packing of silica gel to column (slurry),
here, can I use any solvent for silica gel until reached slurried, or must use only hexane. thank you

[@rahmadinta3315]

Wowww amazingg

[@cello9877]

Claude Debussy's Arabesque no. 1 is a plus to this video :))

[@gerpis]

awesome video! i am happy that my taxes go to support programs like this one.

[@NAZIMUDDIN-px4rm]

good

[@MRFACJJJ]

I liked the video

[@tinellebutcher82]

u want personal and

[@IlmiMulhid]

IGNOU BSCBCH 😅