Performing Column Chromatography
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- čas přidán 9. 03. 2023
- We just learned about thin layer chromatography, but a much more industrially relevant technique is column chromatography. This is how we will separate large quantities of compounds in a mixture by virtue of their differing polarities. It follows the same principles as TLC, but we are pushing compounds down a column of silica gel rather than up a TLC plate. Let's check it out!
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I was shown around the department at the start of my biology Ph.D. first thing I did in that laboratory was accidentally knock over someone’s column chromatography experiment.
It is ok friend we are all human
Prof Dave warned you to use TLC. Lab gear has feelings and needs tender loving care.
Oooooh bad!
пиздец
Oooo Noooo
Thank you! I am so happy that you made this playlist. I am looking forward all the spectroscopies and mass spec. I love to see all the lab procedures through theese videos and their applications in real life analyses. Thank you Professor Dave ❤
Oh my god. I am currently a PhD student in ochem and I didn’t actually learn what a column is (my undergrad was not quite on the level of most institutions). My current research lab has an auto column but I’m also just very early in my research so I’ve only used it once and don’t quite understand the theory if it. This is my first time seeing the theory and it truly reminds me so much of why I love chemistry. So interesting!
It’s because of you and a few other chemistry channels I follow but I do actually get home ads for lab equipment pretty often
Thank you for showcasing the basics of this technique and ending by displaying the latest advancement in this field :)
Interesting video. I used to run flame ionization chromatographs to analyze hydrocarbon gas samples.
One point to add, please dont manipulate silica without a respirator, its a inhalation hazard, especially doing the wet technique.
Class 11th experiment teacher said do it your self in lab now I understand this❤.
Thanku
Hello teacher, how are you?
Could you explain in detail, if possible, how to select the best column based on silica density? Thanks for the video
Good stuff. You should do a video on GC!
good one❤
Fun stuff!
May I know the type of silica gel used in this video, please? Thanks!
شكرا لك
Thanku 😊
Thermofisher sponsorship? Let’s go
Solid loading team
I usually prefer to add sand before I load the sample. It is less likely to disturb the silica gel level with sand on top.
Thanks very much for a good example of column chromatography. Can you tell a concentration of methyl red and bromocresol green in your sample? Or suggest a concentration. I need this for laboratory practice for students.
Awesome
I’ve done 100s , possibly 1000s of columns, but nothing beats doing cryogenic columns over 24-48 hours with sensitive precious metal compounds.
lysergic acid is much more fun
that's cute, when do you discuss HPLC?
it's my favorite.
Could you please explain why the green compound eluated first if its more polar and had to stay longer on the polar silica gel?
Good
Thank you. your videos are very useful and interesting. just please speak in lower speed. so that people like me that English is their second language will understand better. Thank you Professor.
Start a paleontology series
Lamont taught me that the first channel you turn on in the morning ought to be your occupation!
thank you very much. quick question: the solvent that is used to dissolve the compounds (6:59), is it same as the eluent?
So you could do that. keep in mind that you really want to use a solvent where your sample is fully dissolved in as little of solvent as possible.Most of the time it doesnt really matter if you use some other solvent here, as its getting diluted so fast in the column that it doesnt really matter.
@@Noldor97 thanks a lot.
URGENT question, 25 mL of solvent was added to what mass of the silica gel powder?
Ohh... Column Chomatogaphy... The fear of every person which does synthesis...
what do you mean? ding columns is great:D
If bromocresol green is more polar then it must be slower than methyl red but in this practical why the green colour moves faster than red????
Sometimes you are Badass, sometimes Dick, you are non other but professor David.
may I use pieces of this video for a youtube training seminar? I will of course credit you and fischer. as well as link your channels.
Yeah if you're just playing the video on a CZcams player of course
@@ProfessorDaveExplains thank you!
Please, please, don’t just turn the tap on any burette with one hand! You can put a lot of strain on the thin tubing joint between the tube and the stopcock. If it’s just a little stiff you can break the stopcock off spilling solvent everywhere let alone the risk of glass fragments…
And yes, that’s from experience…
The problem is that producers of beads like thermifischer unfortunately don't say what is inside the slurry of the beads and that makes sometimes problems. It's extremely expensive too
Of course they say what is in it. Why would you think that people don't know the composition of silica gel?
Boy you can't get no higher than this!
Please what is the need for the sand?
Okay so: sand at the bottom to prevent silica from getting in your samples that you collect.
Sand at the top to prevent to disrupt your silica when you are readding your eluent
What kind of sand?
normal clean seasand. you can buy that at every chemical supplier
When working with dry silica do it under the hood!!
I loved teaching uv because of trig applications and anti racist history examples both.
First
Two very unpopular technical facts in science.
Dave are you ever going to get more educated or will you remain a high school teacher for your career?
Um, I'm not a high school teacher, I'm a science communicator, and no, I'm done with school.
Hahahaha
Crap