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Setting Up a Temporary Immersion Bioreactor System (Episode 1)

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  • čas přidán 30. 12. 2020
  • Temporary immersion bioreactors are used to grow plants faster than traditional tissue culture. In this series of videoes, we are experimenting using different plants to observe which ones are better to grow in temporary immersion bioreactors or traditional tissue culture.
    We are Plant Cell Technology. We create innovative solutions for commercial and research plant tissue culture laboratories and growers.
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Komentáře • 51

  • @FA-ft9sq
    @FA-ft9sq Před 3 lety +2

    Subscribed and commenting to say that yes, please do the diy bioreactors. Im eager to try making them myself.

  • @andreacarreno4290
    @andreacarreno4290 Před 3 měsíci

    Hi Francisco, could you please explain with more details the connection of the solenoid in the system? Thanks

  • @danieljheelan5256
    @danieljheelan5256 Před rokem

    Hi Francisco,
    Here you have only one solenoid valve.
    When it activated and with what pump.
    Also, why there are three inputs/outputs and not only 2.

  • @lacsacochagrowers8137
    @lacsacochagrowers8137 Před 3 lety

    Hi there, very interesting, please where Ican get the supplies? Thanks

  • @dejkho695
    @dejkho695 Před 3 lety

    List component part of tibs reactor plase?

  • @Krammer1628
    @Krammer1628 Před 3 lety

    Thanks for the video! Do you have available the list of parts necessary to build the bio reactor?

  • @danieljackson4266
    @danieljackson4266 Před 2 lety

    Did you do a video on building TIB? I am trying to figure out how to do this myself now using bottles that I can recycle.

    • @PlantCellTechnology
      @PlantCellTechnology  Před 2 lety

      Designing your own TIB can be a messy, labor-intensive, and costly process. Why don't you give a try to Biocoupler TIB?

  • @ctm75
    @ctm75 Před rokem

    Thank for the awesome videos! May I ask what brand those racks are? They look really nice!

    • @PlantCellTechnology
      @PlantCellTechnology  Před rokem +1

      Need to ask Francisco about these. Will let you know when he responds. Thanks for your patience.

    • @ctm75
      @ctm75 Před rokem

      @@PlantCellTechnology Thank you so much! Loving your videos!

    • @PlantCellTechnology
      @PlantCellTechnology  Před rokem

      @@ctm75 Thanks! I've already sent your question to Francisco. I'll let you know as soon as he responds.

  • @lowatsl6387
    @lowatsl6387 Před 3 lety +1

    Can you give information about lighting you use? Kelvin,wattage flouracent or led? Which one you suggest?

    • @PlantCellTechnology
      @PlantCellTechnology  Před 3 lety +2

      TC plants don't require a lot of light. I'm using cheap LED shop lights from Amazon. 20 Watts, 2,200l lm, 6500 K. www.amazon.com/Barrina-Integrated-Fixture-Utility-Electric/dp/B07DNPL2VC/ref=sxts_sxwds-bia-wc-nc-drs1_0?crid=JT6G94GLYJ6M&cv_ct_cx=barrina+led+shop+light&dchild=1&keywords=barrina+led+shop+light&pd_rd_i=B07DNPL2VC&pd_rd_r=d1c1b2b1-1c46-4159-9055-5017881ee3e7&pd_rd_w=gReaX&pd_rd_wg=UpUbh&pf_rd_p=a64002b9-9c26-4361-b8a1-b0f5a4835670&pf_rd_r=JAJ760YXDVS24VQ590QV&psc=1&qid=1609779334&sprefix=barrina%2Caps%2C200&sr=1-1-38d0a374-3318-4625-ad92-b6761a63ecf6

  • @davebayles9695
    @davebayles9695 Před 3 lety +2

    Hi my friend! I am new to following your videos and I think that you are excellent I like your professionalism. I have a request and I’m hoping you can help. In California Cannabis is legal. It would be great if you could go over a solution for growing the stems and leaves. And also a solution for growing the roots. This would be nice when you have limited space for making clones from mothers. The other option is possibly doing the video as hemp it seems that the mixtures are the same. I have to mixing designs and would be happy to send them to you if you’d like to do the video.

    • @PlantCellTechnology
      @PlantCellTechnology  Před 3 lety +1

      I'll make a video on Cannabis/hemp tissue culture in the future. It will take a while as it is not legal in Tennessee, and the permits to grow hemp may also take some time.

    • @davebayles9695
      @davebayles9695 Před 3 lety

      How about the mixtures using A tomato plant as the explant rather than temp for canvas?

  • @HSCLeo
    @HSCLeo Před 3 lety +2

    Where can I purchase these bioreactors from? Thank you! Great video has been so helpful!

  • @salisali180
    @salisali180 Před rokem

    Please sir, I have research about this reactor. Please help me by giving me enough information about it

    • @PlantCellTechnology
      @PlantCellTechnology  Před rokem +1

      We have a better solution than this reactor, which is Biocoupler-the simplest temporary immersion bioreactor. If you want to try, check out this link: www.plantcelltechnology.com/biocoupler-tm-glass-set/

  • @dejkho695
    @dejkho695 Před 3 lety

    How buy air fillter at where ?

  • @jeipondaelevens1196
    @jeipondaelevens1196 Před 3 lety

    Is there any courses available for this to learn from you?

  • @harishs2181
    @harishs2181 Před rokem

    Can we propagate rose in TIB?

    • @PlantCellTechnology
      @PlantCellTechnology  Před rokem

      TIB is mainly used for the multiplication of plants. So, you can establish it in tissue culture and multiply them in hundreds and thousands in the TIB system.

    • @harishs2181
      @harishs2181 Před rokem

      @@PlantCellTechnology
      where to buy this technology and protocols

    • @PlantCellTechnology
      @PlantCellTechnology  Před rokem

      @@harishs2181 You can find some protocols over the internet. We also have blogs containing TC protocols of some plants, taken from a research paper. Here's the link: www.plantcelltechnology.com/blog/

  • @kelseystarr3564
    @kelseystarr3564 Před 4 měsíci

    Where can someone buy these kits for the TIBS? I can't seem to find any like this..

    • @PlantCellTechnology
      @PlantCellTechnology  Před 4 měsíci

      Francisco purchased some items from a local store and some online. However, if you are interested in a temporary immersion bioreactor, why don't you give it a try to Biocoupler? It's the simplest TIB, taking less space in your lab, and is simple to operate. Check it out here: www.plantcelltechnology.com/biocoupler-glass-set/?_ga=2.225713242.1379241722.1711331365-654510305.1688485895

    • @kelseystarr3564
      @kelseystarr3564 Před 4 měsíci

      @@PlantCellTechnologyI’m certainly considering it. I just need to gather more information first. I need to better understand how they work, so I’m in information collecting phase! I am only in the lab 3 days a week, so I guess I’m a little concerned about the manual nature of the biocoupler and if the explants suffer or dry out with too much time. But like I said, I still have much to learn how these really work.

    • @dragonsimon1
      @dragonsimon1 Před 4 měsíci

      @@PlantCellTechnology I would consider the biocuplers you guys sell but they're very costly at over 27 usd per. Very convenient but hoping the cost per unit will be reduced in the future.

    • @PlantCellTechnology
      @PlantCellTechnology  Před 3 měsíci +1

      @@dragonsimon1 We totally understand your limitation. We often have giveaways and sales on our products, so never miss that, as it will be extremely useful for you. Do you follow us on Instagram? Generally, we post such news on Insta, Facebook, and Linkedin.

    • @dragonsimon1
      @dragonsimon1 Před 3 měsíci

      @@PlantCellTechnology yep been following you guys since the beginning! Hoping the next sale is soon 😁

  • @erwansan
    @erwansan Před 2 lety

    Are you selling bioreactor container?

    • @PlantCellTechnology
      @PlantCellTechnology  Před 2 lety

      We have a Biocoupler (TM) temporary immersion reactor, which is one of the simplest TIB available so far.
      If you are looking for that. check out this link: www.plantcelltechnology.com/biocoupler-tm/?_ga=2.150518710.1635719926.1648361640-137788240.1591809399

  • @tallybeaverman9462
    @tallybeaverman9462 Před 3 lety

    What does a Bio Reactor do?

    • @PlantCellTechnology
      @PlantCellTechnology  Před 3 lety +1

      A bio reactor is a key component of tissue culture, supporting the growth of plants in its environment.

    • @PlantCellTechnology
      @PlantCellTechnology  Před 3 lety

      Most plants grow faster in a temporary immersion bioreactor than in traditional tissue culture. Up to 10x faster with some plants. Also, plants in temporary immersion bioreactors are easier to acclimate to soil as then are constantly breathing due to the oxygen exchange.

  • @tulsiramdagur9580
    @tulsiramdagur9580 Před rokem

    एक बायोरीएक्टर पर्चेस करना है।
    क्या कीमत है

    • @PlantCellTechnology
      @PlantCellTechnology  Před rokem

      We don't have the traditional bioreactor systems, however, we do have the simplest temporary immersion bioreactor )Biocoupler) used at the multiplication and rooting stage of tissue culture. If interested, learn more about its features and price here: www.plantcelltechnology.com/pct-store/

  • @danieljheelan5256
    @danieljheelan5256 Před 2 lety

    Hi Francisco,
    I am interested in getting the list of parts to built my own Bioreactor.
    Please update me.
    Message sent on Messenger.
    Thanks
    Daniel