How to interpret results of Microbiome analysis | alpha beta diversity
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- čas přidán 15. 04. 2023
- #interpretation #microbiome #diversity
In this video, I have shown how we can interpret the microbiome analysis results. I have explained OTU (Oppertional taxonomic unit), alpha diversity, beta diversity, PCA and PCoA, and NMDS analysis.
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This was really informative respected Sir. Love from Japan.
Thanks for message, glad it its helpful
Thanks for message, glad it its helpful
Dear sir, Could you please tell me how you get or design the "sample information sheet". Could you please guide me about the previous procedure of microbiome analysis. I am working on the related to this. Looking for you reply.
Thank you so much .
Thanks saba
Last month I got results from company. I was worried how I can interpret it and put these results in my thesis. Your video help me a lot to understand the basics of this technique. Moreover, I would like to request you to record a detailed video on NMDS analysis. Thank you
Glad if it’s helpful to you. Sure, this topic is already listed down my list. Stay tuned
Talented Pakistani Researchers all over the world 🌍! Thanks for spreading knowledge!
Glad you like it
does the company also mention the kind of sequencing they used like 16s rna sequencing or gun shot sequencing and what about the bioinformatics analysis they use can you please explain?
Yes they also mention kind of sequencing they used
thank you brother.I am new to this so if you could help me with some queries i would be grateful.After we collect sample then do we have to tell lab about the sequencing we want to do or they do it on their own?And also i saw bioinformatics method being used do they perform the bioinformatics analysis also?
U made my day ❤😊tnx sir
Glad you find it helpful
Very informative but could you please make more detailed video about Shannon's analysis ?
Sure i will record soon
Hello Dr. Asif,
Please I have a question about how to arrange the samples appearance on X-axis, however I did in the sample information section, but I need to modify the graph in order to samples, any help please?
Hi can you be specific which graph?
Hello thanks for this video, please did you make follow-up videos for this like you said in the end?
Sure can you clarify what specifically you want me to address?
@@asifmolbio Hello, came to comment again didn't know you replied earlier. I need help on how to calculate Shannon, Simpson, and Chao 1 and how to create a Venn diagram. my result has a little time duration before it's deleted.
Nice video. One question, I want to register on the Majorbio page, but I have problems registering my phone number. I'm from Peru and I tried my country's code but it appears to be incorrect. I hope you can solve my problem, thank you very much.
Login should be created by company you can write email and request then to register for you
@@asifmolbio Oh, thank you so much.
I have a Chinese version but didn't find English version ..any solution
Use vpn, open in chrome and translate with mouse left click. In video, i have done the same
What is the difference between +ve control and -ve control?
In sequencing experiments, positive and negative controls are used to ensure the accuracy and reliability of the results:
1. **Positive control:** This is a sample or substance that is expected to give a positive result. It is used to verify that the experimental conditions are suitable for the detection method and that the system is working as expected. For sequencing experiments, a positive control might be a known DNA sample with a specific sequence that should be detected by the sequencing method.
2. **Negative control:** This is a sample or substance that should not give a positive result. It is used to detect any contamination or background signal that could lead to false positive results. In sequencing experiments, a negative control might be a sample without any DNA, or with a DNA sequence that is not expected to be detected.
In summary, positive controls are used to validate the experimental method, while negative controls are used to detect and prevent false positive results.
It is telling malformed phone number? Please tell me how to sign up
This can be only generated by company.
Can we add our personal data to this website?
No have to upload by company only
Is this tool free for microbiome data analysis for chinese only? It is not accepting contact no while registering. Please suggest the solution
In this video, i did not discuss any tool probably you are taking about SR tool. it accepts emails
@@asifmolbio I am asking about majorbio tool. When I am registering on it requires chinese contact no shows error
Majorbio tool needs official login created by company.
Plz make video that once excel sheet is download from majorbio then how to clean data and calculat relative abundance at differe level like phylum or genus please
just I'm struck with same thing. Is any solution for this.
AoA sir, can I have your wechat please? I’m doing the same work and need some assistance please