Introduction to Colorimeter || Demonstration of Colorimeter Practical || Beer Lambert's Law

Video on Colorimeter and Beer's Lamber's Law - This is video on introduction to colorimeter and HANDS on colorimeter which will be useful for 1st year MBBS as well as to DMLT students for the biochemistry practical.
Video contains
1. Introduction to Colorimeter - 01:00 minutes
2. Beer's Lambert's Law -1:25 minutes
3. Absorbannce and OD- 2:44 minutes
4. Parts of Colorimeter- 4:00 minutes
5. Operational procedure - 5:45 minutes
6. Different types of blank - 6:52 minutes
7. Calculation - 7:25minutes
8. HANDS ON COLORIMETER - 8:39 minutes
Colorimetry
In this technique , the concentration of compound is determined by measuring the intensity of color.
Colorimeter
They are simple instrument which measure the intensity of transmitted light through color solution
Beer’s law
When a parallel beam of monochromatic light is passed through a solution, the absorbance (A) of the solution is directly proportional to concentration (C) of the substance in solution
A = KC
K is a proportionality constant
Lambert’s Law
When a parallel beam of monochromatic light is passed through a solution, the absorbance (A) of the solution is directly proportional to length of the light path (l)
A=Kl

[@roshnibhatt2801]

Thankyou sir for giving all information regarding the colorimeter, now I am totally understood to the how the colorimeter work by two laws, Beer's and labert's law, Thankyou so much again sir👍

[@Biochemistrybasics]

Glad to hear that. Please spare a few minutes and do spread the word about the channel among your batchmates and friends

[@roshnibhatt2801]

Sure sir.

[@kalpanamaheshwari6796]

I admire your persistence and will to share Knowledge. Keep it up. Best wishes..😇

[@kumarvaibhav4937]

Sir, you are awesome.You explained every thing in very simple manner.

[@Biochemistrybasics]

Thanks dear. Please spare a few minutes and do spread the word about the channel among your batchmates and friends

[@SudikshaRawat]

Thank you sir for such a helpful video🙏🙏

[@Biochemistrybasics]

My pleasure dear

[@champadhali6746]

Thank you so much sir🙏

[@Biochemistrybasics]

Most welcome dear

[@shahhannan]

Sir, why is it important that the marking on the covet should coincide with marking on the colorimeter?

[@emory222]

Thanks a lot for this video!☆

[@Biochemistrybasics]

Most welcome

[@abhishekiswalkar3835]

Impressive sir 👌

[@Biochemistrybasics]

🙏😊

[@medstethnepal100]

Superb explanation sir..

[@Biochemistrybasics]

Thanks ranjan

[@nazminsaifi1485]

Superb sir , nicely explained

[@Biochemistrybasics]

Thanks nazmin

[@hazel8537]

Sir BSC nutrition and dietetics or bsc radiology which is best course

[@Biochemistrybasics]

BSc radio

[@hazel8537]

In bsc or MSc in radiology what to do next sir ....? Whether we open own daiognastic clinic or PhD which is better in future ??

[@Biochemistrybasics]

@@hazel8537 you can't open your own diagnostic center. Though you can work in it

[@hazel8537]

@@Biochemistrybasics Sir.....a bsc completed student has very low starting salary. So choose MSc . After completion of MSc what jobs are available sir.....?

[@emishaemi5356]

Thank you sir for you clear explanation and demonstration

[@Biochemistrybasics]

Most welcome dear

[@harshithakr4562]

Tqsm sir, it is very useful to us

[@Biochemistrybasics]

My pleasure harshitha. Please spare a few minutes and do spread the word about the channel among your batchmates and friends

[@asifiqbal1482]

Sir i greatly admire your hard work i want to request you kindly make colorimetrically determination of Bilirubin,alkaline phosphatase,uric acid,sgot,sgpt, practical videos. i am in great need plz sir🙏

[@Biochemistrybasics]

Sure. Will try my best

[@Amritasingh-uk6lf]

Sir thoda Hindi me bhi bta dijiye taki hme bhi kuch samjh me aa jaye please sir .
I request you sir 🙏🙏

[@ashupatnaik8809]

Sir good afternoon.sir I have doubt. That I am using Beacon brand colorimeter for biochemistry test.doubt is that.my colorimeter OD range is 2.00 to -0.31. I 1st gave blank and set zero,after remove blank the OD is 1.69.is it correct working my colorimeter.

[@Biochemistrybasics]

1st you have to set the zero and then you have to measure the od of blank

[@b_h_a_v_a_n_a]

Thanks a lot sir...😊

[@sonimahto677]

Thank you so much sir

[@Biochemistrybasics]

Most welcome dear

[@bhagyashreechaudhari588]

Thank you sir

[@Biochemistrybasics]

My pleasure bhagyashree. Please spare a few minutes and do spread the word about the channel among your batchmates and friends

[@AliReza-xk7if]

Sir,
Your descriptions is so so nice and helpful to understand clearly. Can you make a video of Blood glucose meter please ?
Md Ali Reza

[@Biochemistrybasics]

Thanks dear for the appreciation. Sure will do it

[@sakshigoyal6921]

Helpful video

[@Biochemistrybasics]

Thanks dear 🙏☺️

[@sakinakumre7508]

Thank you so much sir nice video 🥰🥰

[@Biochemistrybasics]

Thanks सकीना.

[@sakinakumre7508]

@@Biochemistrybasics you are welcome 😇sir

[@upendrasingamsetty7526]

good explanation thank you

[@Biochemistrybasics]

Thanks Upendra. Keep loving biochemistry and do spread the word about the channel wholeheartedly

[@upendrasingamsetty7526]

@@Biochemistrybasics sure sir thanks for response😄😁😊

[@singtothecreator2720]

Great sir

[@Biochemistrybasics]

😇

[@aasma_pathan]

Why we are using cuvett made up of glass in colorimeter and quartz in spectrophotometer

[@navitameena6832]

Egg,milk, analysis of blood , analysis of bile,HBA1c

[@navitameena6832]

Sir ji yeh practical bhi upload kijiye

[@rajdiagnosticcentre9191]

Sir pls mention zero settings through technical engineer

[@Biochemistrybasics]

Okay

[@vineets.h8784]

Thank you sir it helped me in university exam

[@Biochemistrybasics]

Wow. Glad to hear that

[@sree7834]

Thank you so much sir..

[@Biochemistrybasics]

My pleasure sreevidya. Please spare a few minutes and do spread the word about the channel among your batchmates and friends

[@sanskritidutta]

Thank you so much sir❤️

[@Biochemistrybasics]

My pleasure संस्कृति. Keep loving biochemistry and do spread the word about the channel 😊

[@bhagatanijayshree6284]

Your all video are helpful for us

[@Biochemistrybasics]

Glad to hear that jayshree

[@nehasuthar5500]

Very nice sir👍 sir can I add beer Lambert law in spectrophotometry in slides....?

[@Biochemistrybasics]

Yes you can add

[@nehasuthar5500]

@@Biochemistrybasics okay sir👍

[@zaramobeen6646]

thank you sir!!!

[@Biochemistrybasics]

Most welcome

[@shreerajpaudel8257]

plz make vdeo on Ion selective electrode

[@Biochemistrybasics]

Okay. Sure

[@ajayksaw5558]

Digital Colorimeter me test Kaise karte h sir Jee test karke procedure blood sugar Ka test

[@Biochemistrybasics]

Take the OD of blank standard and test and enter the value in formula and calculate the total amount of sugar present in the solution

[@asatident3555]

❤️❤️

[@Biochemistrybasics]

❤️

[@usmanakhtar3992]

Thanks

[@powerisme7551]

Why different colour filter is used as complementary?

[@Biochemistrybasics]

A complementary filter allows only the color of light that the solution absorbs most to pass through. By eliminating other wavelengths, the colorimeter focuses on the interaction between the light and the colored molecule.

[@mominsonu8986]

Thanks sir❤️

[@Biochemistrybasics]

❤️😊

[@kantimusic558]

Thinku sir

[@Biochemistrybasics]

Most welcome dear

[@Yash-uo6tz]

Sir why we set the reading at zero after inserting the cuvette?

[@Biochemistrybasics]

Zeroing a colorimeter before taking a reading is important because it allows for any background color or light to be accounted for and removed from the final measurement.
When a colorimeter is zeroed, it essentially establishes a baseline reading of the blank or clear solution, without any sample present. This baseline reading accounts for any color or light that is naturally present in the system, such as ambient light, impurities in the reagents, or any residual color from a previous sample. By zeroing the colorimeter, these potential sources of error are accounted for and eliminated from the final measurement.
Once the colorimeter is zeroed, the sample can then be added and its absorbance or transmittance can be accurately measured. This ensures that the final reading is a true representation of the color or concentration of the sample, without any interference from background color or light.

[@happyzone1396]

TQ U SIR

[@Biochemistrybasics]

My pleasure. Keep loving biochemistry and do spread the word about the channel

[@pushpapuree990]

Sir plz batadijiye kinetic test measurement konsa laboratory machine mea difficult hota hea

[@Biochemistrybasics]

In colorimeter it's measurement is difficult. Better do it in semi automated biochemistry analyzer

[@pushpapuree990]

@@Biochemistrybasics thanks alot sir 🙏🙏🙏🙏🙏🙏🙏🙏🙏🙏🙏

[@abhishekshyamgarg336]

Sir kya colorimeter me sare biochemistry test perform kiye ja skte hai Erba reagent ke sath.

[@Biochemistrybasics]

Yes. Almost all except tests which require multiple calibration

[@abhishekshyamgarg336]

@@Biochemistrybasics thanks sir ji

[@iftekarhossain8397]

I habe monozyme innova 1000 colorimeter. 520 filter me zero settings thikk se nhi ho rahahai

[@Biochemistrybasics]

Please do check with biomedical engineer

[@danmuanching83]

How much blank we keep in the tube....

[@Biochemistrybasics]

One ml

[@ridwansalifu6987]

Thank you

[@ridwansalifu6987]

I have a practical exam tomorrow and this video contained exactly what I needed

[@Biochemistrybasics]

@@ridwansalifu6987 Most welcome dear. All the very best for the exam

[@SyedaNishath007]

Sir please post video on principles of estimation of urea by different methods and standardisation and estimation of urea by dam method

[@Biochemistrybasics]

Yes syeda will do. Video on estimation of glucose by GOD POD is there. You can watch it.

[@navitameena6832]

More videos upload sir ji

[@Biochemistrybasics]

Please see "practical" playlist.... All video are there

[@navitameena6832]

Thankyou so much sir ji

[@Biochemistrybasics]

😇🙏

[@docactor3637]

Sir please post video on sgpt

[@Biochemistrybasics]

Sure. Will

[@docactor3637]

Thanku sir
Your video help in our practical viva🙏

[@Biochemistrybasics]

@@docactor3637 glad to hear that. Please spare a few minutes and do spread the word about the channel among your batchmates and friends

[@docactor3637]

Ofcourse sir

[@Adibafarha]

What is mobile phase and stationary phase in colorimrtry

[@Biochemistrybasics]

There is no such phase in colorimeter. It comes in chromatography

[@rohitkumarsangar1511]

In Kutch

[@navitameena6832]

Unknown compound p bhi bnaye

[@Biochemistrybasics]

Okay

[@navitameena6832]

Thankyou so much sir ji

[@phoenixmt8657]

Can I use Euro Diagnostic Biochemical Reagents in Colorimeter

[@Biochemistrybasics]

Yes you can

[@Biochemistrybasics]

But read kit insert before using it

[@phoenixmt8657]

Thank you sir..but I have another question regarding preparation of Working reagent especially in urea determination by berthelot method.Can you please explain

[@Biochemistrybasics]

@@phoenixmt8657 what's the confusion

[@phoenixmt8657]

I need only a small quantity preparation of working reagent to per patient test.From the kit insert,Working reagent must be prepared by adding all the enzyme reagent bottle(3ml) to Buffer reagent(60ml).and it is stable for 16 weeks.My testing load is very low.I need to prepare working reagent per patient.But I don't know the proportion of mixing.

[@rohitkumarsangar1511]

Sir ap Adani gaims.me padha te ho

[@Biochemistrybasics]

Yes.

[@Suresh_Jay]

plz work on audio quality

[@Biochemistrybasics]

Noted 😇

[@Dr.HareenKalyan]

B S T FULL FORM?
VOICE IS NOT SO CLEAR

[@sadhnachaudhary3146]

Sir pls aap procedure bta dijiye

[@Biochemistrybasics]

It's explain at the end of the video

[@amarSINGH-dd6uq]

Hii

[@Biochemistrybasics]

Hi

[@Ss-um1fq]

woh lab coat mat peheno sir warna aapke subscriber bhi gir jayenge

[@hussaintahir3383]

Thank you sir

[@Biochemistrybasics]

Most welcome dear