How to use Pierce Protein Concentrators for 5-20 mL sample volumes

5-20 mL Pierce Protein Concentrators. Learn how to concentrate, desalt or buffer exchange your protein solution using Pierce Protein Concentrators (PES) for sample volumes from 5-20 mL.

[@user-en1rm4lb3v]

Can we use this multiple times for same sample. Means I have to concentrate same protein with same buffer after 1 month then can I store this? If yes then how we store it?

[@thermofisher]

Hello User-en1rm4lb3v , Thanks for your question. Can you please reach out to our technical support team at thermofisher.com/askaquestion. They would be the best team to assist. Thank you!

[@sci_djw7670]

How much volume would I get back from 10 ml of cell culture supernatant using the 3kDa cutoff?

[@thermofisher]

Hello, Thank you for your question. Please contact our technical support team at thermofisher.com/askaquestion for additional information. Thank you!

[@MrABHILEK]

Depending on your desire.U have to stop accordingly

[@yinzewu5340]

What is the " printed sample window"? For fixed angle rotors, it's membrane facing outwards or membrane parallel to the radius? Thanks.

[@petroswes5413]

I had the same problem. czcams.com/video/L2U9ev85vgs/video.html This video might help you.

[@shaolinma1273]

Great video. How many times for buffer exchange is sufficient? Thanks

[@thermofisher]

Thank you for your question. Please contact our technical support team at thermofisher.com/askaquestion so they can assist. Thank you.

[@dennisgitaunjuguna8610]

How many times these concentrators can they be
be re used

[@thermofisher]

Hi Dennis. Thanks for your question.
If for example, you had 35 mL of your sample and wanted to spin down all of it, you are welcome to spin down smaller volumes until all 35 mL of your sample is spun down.
However, if you are referring to re-using the concentrator for different sample preps, unfortunately, these concentrators are one sample per use.
For any additional questions, please contact thermofisher.com/askaquestion. Thank you

[@annasebastian2504]

Is it called thin filters??

[@thermofisher]

Thank you for your question. Please contact our technical support team at thermofisher.com/askaquestion for additional information. Thank you!

[@MrABHILEK]

No. it’s molecular weight cut off membrane