Concentrating Proteins Using Amicon Centrifugal Filters

[@CopperKettle]

Thank you, it was interesting to see it in real life. I was translating a text mentioning such filters.

[@th3s3raph]

thank you very much. i am using it to concentrate VLPs. this was very helpful.

[@4Getf00l]

Thank you for the demonstration. I was completely confused.
However, I have a question, is it necessary to dilute the protein in the initial step ? or the existing protein solution can be used as is?

[@natassiacorrea7249]

Thanks!

[@tartanhandbag]

Do The Right Thing

[@bann3t]

Is this sterile

[@shirazi2007]

The manual says that they're not sterile. I'm not sure if we can autoclave them as the maximum temperature for the membrane is 40 C.