How to Germinate Seeds with Hydrogen Peroxide: A Step-by-Step Guide
Vložit
- čas přidán 1. 09. 2021
- Are you struggling to get your seeds to germinate? Hydrogen peroxide can be a great tool to help speed up the process and ensure successful germination. In this video, we'll walk you through the steps of using hydrogen peroxide to germinate your seeds. Get 10%OFF the tissue culture products you need using my code: www.plantcelltechnology.com/?...
First, we'll explain why hydrogen peroxide is beneficial for seed germination, and what it actually does to help the process along. Then, we'll show you how to prepare a hydrogen peroxide solution and soak your seeds in it before planting.
We'll also provide tips for choosing the right concentration of hydrogen peroxide, as well as how long to soak your seeds for optimal results. Finally, we'll discuss some common mistakes to avoid when using hydrogen peroxide for seed germination.
By the end of this video, you'll have all the information you need to successfully germinate your seeds using hydrogen peroxide. So grab your seeds, some hydrogen peroxide, and let's get started!
Don't forget to subscribe to our channel for hundreds of free educational plant tissue culture videos and be sure to mark your calendar for our upcoming Houseplants Tissue Culture Master Class with Francisco in Washington DC. This is a unique opportunity to learn from the experts and take your plant tissue culture skills to the next level. Join us and discover the amazing world of plant tissue culture today! plantcelltechnology.com/master-classes/
Follow us on social media!
INSTAGRAM: / plant.cell.technology
FACEBOOK: / plantcelltechnology
LINKEDIN: / plant-cell-technology-...
TIKTOK: / plantcelltech
TWITTER: twittter.com/plantcellnews
We are Plant Cell Technology, a leading plant tissue culture industry organization since 1995. We develop, produce, and market innovative solutions for global plant tissue culture operations and hobbyists. Our well-researched, certified, and trusted products keep your cultures healthy and contaminate-free.
rare house plants, tissue culture, master class, carnivorous plants, plant propagation, sustainable, growth, care tips, update, houseplant trends
#houseplants #rareplants #tissueculture #masterclass #carnivorousplants #propagation #sustainable #update #plantcare #plantgrowth
#PlantCellTechnology #TissueCultureMasterClass #TissueCulture #MicroPropagation #Plants #Labratory #Lab #TCLab #LiquidMediaCultures #MicroBiology #Cultures #LiquidMedia #TC #planttest #tissueculturetest #protocols #tissuecultureprotocols #coffeechat #tissueculturequestions #tissuecultureanswers #planttissueculture #plants #plant #propagation #learntissueculture #tissuecultureclass #tissuecultureeducation
#PlantCellTechnology #TissueCulture #Propagation #MicroPropagation #Plants #Labratory #Lab #TCLab #LabEquipment #LiquidMediaCultures #MicroBiology #Cultures #LiquidMedia #TC - Věda a technologie
Just tried this on seeds I've had trouble germinating and I have at least 40% germination on a variety of seeds in 2 days. Thank you!
Great to hear!
@@PlantCellTechnologyhallo wat is mixture for volume 10 hydrogen peroxide
That's awesome. I've never heard of this method
Awesome man.
Thank you! From Baudette Minnesota
Thank you! Very interesting and informative.
Great, you enjoyed it!
Great explanation, thank you. I’ll will try this method today.
Amazing. Go for it. Let me know how did it go.
Great info. Thanks for your efforts.
Thanks for your comment!
Beautiful 🙌
Thank you 👌🏿
I have a few difficult seeds. Will try. Thank you for such a detailed and logical flow with demonstrations.
Hey,
Thank you so much for your lovely feedback. Let me know how the process turned out for your seeds.
Happy culturing!!
I concur with ur sentiments here
thanks dude.
Hallo
A question
You mentioned : about 2 days soaking
Does that mean exactly 2days or longer
I want to use it for cactus seeds
Thanks in advance
Thanks so Dear friend
It is good we will deal with
Glad you found in helpful!
Hi do you place your seeds in the tube that has h202 in a shaded area or close to a window with some light ? Tks
thank you for all the info! i follow here and instagram!
Happy to hear that!
I will start my peppers seeds soon with paper towel method. Since I have some bit difficult ones, I looked for ways to speed thing up and thought about soak them in 3% H2O2 for about 12h...but now I will directly moisten the towel with aprox 0.3%. Thank you
Do you grow pepper plants in tissue culture? I'd love to get it to work. I've had one pepper seed survive so far.
@@youtubemusicandlyrics4677 I only start them im tissue. Its acctualy pretty simple: Place seed on a wet tissue or makeup pad. Put in a ziplock bag and let in a warm space. Exept for some very hard germinating ones I wouldnt recomend peroxide tbh...kinda fucked up with it last year
@@youtubemusicandlyrics4677 Theoretically, you can grow every plant in tissue culture.
Did if work?
Hello is this h202 stabilized?
Thanks
Can I do this with orchid seeds?
Can you use it to grow cactus?
Yo, and what agar recipe for such seed germination? Can I be successful with simple MEAgar like for mushrooms?
Nice
How long I should soak LICUALA palm SEED in hydrogen peroxide before potting them ?
Nice video, only thing I was left wondering is how much water/peroxide solution was added to the seeds at the start. Just enough to be moist or like a drop?
1 part every 9 part water
Just enough to cover the seeds
Do you use sterilized water, Distilled water?
I put 15 seeds in a cup of tap water and splashed a little peroxide in there, we'll see what happens
@@danuuuuuuuuuuuuuuu in his newer video he says 1 part peroxide to 19 parts water.
czcams.com/video/ErMyL8ag6go/video.htmlsi=lbCZHVFGZ6yQhB5V&t=44 It's very tough to get information when people who claim to have answers change their information. 9 parts vs. 19 parts is a HUGE discrepancy.
When you transfer the seeds to the germination cup do you drain the water and put only the seeds in or the water as well or just a little water and do you have any air holes or just close the cup.
Thank you for the video. I am trying some 13 year old seeds that I have had no luck with traditional ways.2008 oregon cup winner medicine woman and Mendocino purp. Hopefully i can get them going again!
after the seeds are germinated, I just transfer only the seeds to the culture medium to induce growth.
This is extremely awesome... I can use some assistance here, as I am having poor germination with some Camelina seeds... In fact, one project failed already, and this video just pumps new life going forward. Ques: can I drenched my growing medium in plants trays...say 128 cells instead of the seeds, trying to plant one acre, and I'm sure u can appreciate doing it as in the vid, it can get quite labourious...can you advise...thanks a mill.
Hi,
we have forwarded your question to our lab director Francisco. So, we will get back to you as soon as he responds.
Camelina germination viability drops significantly after a year. One of my grad students has a project on winter oil seeds, and camelina is one of the crops used in her project. We noticed that the germination is poor with older seeds.
We are also having issues with plants flowering too early or not making it through the winter.
thank you
Ok, pardon me if I miss any data in this video, can I ask you how long does the seeds take in H2O2 solution in sterilize step?
here we used h2o2 for the seedling growth. Not for the sterilization.
I want to do this method I just did a HP seed germination with 1 tablespoon of HP with 8 Oz water and 1/3teaspoon of sugar then planted in soil this is 6th da and only 1 is coming up planted 6.
I also just took 6 seeds an planted them in soil and the petri dish method which was basically just a wet paper towel in a container.
Natural has 2 already up and petri has3 up and 2 more trying.
These are tomatoe seeds I had high hopes for the HP.
I see your HP method is different where you throw them into tissue culture medium where do I get that from and do I need a sterile box?
Yes, you need to maintain complete sterile conditions to perform tissue culture techniques. You can get all the materials needed for the process here: www.plantcelltechnology.com/pct-store/
This is great, what is inside your plastic cup? Is that a gel?
Yes! Media with gel.
@@PlantCellTechnology you said it’s canola? Can I order this medium from somewhere or make it myself?
@@Dirtyshinobi Yes, in one cup it was canola! You can order the basic MS media and agar or gellan gum using this link www.plantcelltechnology.com/gelling-agents/. However, you will have to prepare the media mixture to grow explants by yourself.
We are looking fotissue culture cups made from plastic or moly ethelene sililar to what you used in your videw
You can purchase them from a local store. Just ensure they're autoclavable.
Very interesting!
What book about TC do you recommend?
Plants from test tubes is the only one
You can go for any of these:
1. Plant tissue culture: theory and practice
2. Plant cell and tissue culture-a tool in biotechnology: basics and application
3. Plant tissue culture: an introductory text
4. Plant tissue culture: techniques and experiments
I have that book that I got from my local library. Libraries can access books from all over the state that are in the library compatible networks. 👍 Free!! 😉😊
❤
What is in the little pastic cups besides the terminated seeds. What are the living in and making them grow?
From hydrogen peroxide, seeds are transferred to cups containing tissue culture media to support the growth of shoots of the plant.
Where can I get the clear gel to put the seeds in? Can I make it? What’s it called ?
Do you need agar or Gellan gum? If yes, then you can purchase using the link: www.plantcelltechnology.com/gelling-agents/
Should you transplant when there is roots only or do you wait until there is some leave in the tube?
When you see the radicle coming out of the seed, transfer it to the culture media. Because plants need other nutrients to develop organs.
When taking the germinated seeds from H2O2 and transplanting it to culture media -What does this culture media contain for seeds ? Just MS
Do you think this concentration 1/9 parts will work to sterilize Drosera seeds for in vitro germination?
For me using 0.3% worked for the plants that I tried to grow. But the concentration differs based on the type of the plant. So, you might want to check it once before starting the TC processes.
Placing germinated seeds into agar- what media does the agar contain? Is there MS in or plain agar. I want to use Full Ms media for my germinated seeds
It's MS media. Plants need nutrients to grow. So, placing them alone in agar won't do any benefit.
you think 1oz peroxide to 2 oz distilled water is not enough water? i noticed most people use that ratio. what is better with 1oz to 9 oz water ratio?
I have used 1:15 for years. I give the seeds a 30 minute soak, then plant them and dribble a little of the peroxide solution right into the planting hole. H2O2 turns to H2O rather quickly, so there is no concern about continual soaking in H2O2.
What is way to do plumeria plant to tc!!
You can find the protocol for tissue culture of any plant by googling "tissue culture of plumeria plant" or any other plant you want.
Did you try this on nepenthes seeds? not sure which way to steralize these seen a few methods 👍
No. currently, didn't try on that plant.
Do u can germinate venus flytrap or other seeds in it, 0.3 hydrogen peroxide use directly or diluted? Plant cell technology
Can you germinate maple seeds, cedar seeds etc using this method. They need cold stratification - so can I soak them in the 0.03% H2O2, leave them in the small tubes and cold stratify them in the fridge?
I would separate the steps
any luck? i got maple and cedar seeds as well.
i had the maple seeds in the fridge for a week on a damp paper towel and they started to grow mold. i guess i didn't ventilate the bag good enough.
they're now soaking in peroxide. i separated some before hand to try different methods and those ones are okay but still not growing.
What culture tissue media are u using and is it pre made
It's not premade. Francisco developed it in his lab for the plant he was working on.
What's that gel substance in the cups?
It's a tissue culture media containing agar.
@@PlantCellTechnology thanks, just learning about this from your other videos
@@sidekick3rida Glad, we're able to help you in your tissue culture journey. If there's anything that you can't find in our videos, check our blogs too which has a vast range of tissue culture content on various topics.
Here's the link in case you're interested to check it out: www.plantcelltechnology.com/blog/
Can I use this on Lavender seeds? Last summer, all my lavender seeds did not germinate but all my other seeds germinated within 1- 2 weeks.
We didn't give it a try on Lavender seeds. But, maybe you can work with a few of them to see how it goes.
What concentration of hydrogen peroxide to use? How much dilution should be made?
#1 a. 1-Part of Hydrogen peroxide solution (10 milliliters or 3%) #2 b. 19-Parts of Sterile water (190 milliliters) 3# c. 1-Percent of Sugar or Molasses Blackstrap unsulphured. Hope this helps, and Remember You've Got This!
Have you tried this on Citrus?, specifically C-22
N. Not yet.
@@PlantCellTechnology I'll start some protocols and let you know
@@rsigrowers3587 Wonderful!!
Can this be used on seeds that are very old? I recently found a collection of seeds that I though I had lost during a move to a new home over 10 years ago, and would really like to save some of these seeds.
We didn't give it a try on such seeds yet. But, maybe you can work with a few of them to see how it goes. It only requires sugar, H202, and water.
@@PlantCellTechnology Will do. I can send some of the extras to you for an experimental video if you'd like. These seeds are from 2012, so they are quite old.
hello, thanks for this experience. I have H2o2 at 12% , how many ml of H2o2 for 1 liter of water please ?!
you have nicola tesla on your profile picture and you cant do simple math? :D give it a try at least, it's not that hard... your final solution is supposed to be 1% h2o2
C1V1=C2V2
Could you place these directly into a bioreactor system rather than agar media?
No. Seeds need solid support to breath, grow, and develop in the environment.
@@PlantCellTechnology thank you for the information, be awesome if you could though lol
@@jasonwalters7896, Of course, Jason! Let us know if you have any other queries. Happy to help!
Is it good for pepper seeds too?
We didn't give it a try on Pepper seeds. But, maybe you can work with a few of them to see how it goes. It only requires H2O2, water, and sugar.
@@PlantCellTechnology I'll try nextime thanks
Sure! Do let us know how it goes for you! @@kimimashintv3285
@@PlantCellTechnology I tried it today I'll update you about the progress
Great news!!@@kimimashintv3285
for how long i have to soak seeds in the h2o2 solution before plant it on a media?
Until you see the radicle growing out of it.
@Rick Sanchez C-137 Hey, I am forwarding your questions to Francisco. I will let you know the answer as soon as he responds. Thanks for your patience.
@Rick Sanchez C-137 Thank you so much, Rick. We are trying our best to bring excellent TC content, product, and services to our culturists. Thanks for being an integral part of this community.
@Rick Sanchez C-137 That's amazing. Happy culturing!
Is this method advisable for berry seeds
Need to confirm this with Francisco?
Hi, how much hydrogen peroxide do you add to water ? thanks
You need to use 1 part hydrogen peroxide and 9 part water.
how we should transfer seedlings from tube to media without getting contaminated ?
You need to do it under the laminar flow hood. And ensure everything is sterile.
So once seeds root, place them into a rooting media?
Yes, when you see the radicle coming out of seed, transfer them to culture media for their organ development.
@@PlantCellTechnology thank you
Just clarifying......the solution you made is 9 parts water and 1 part hydrogen peroxide ?
Yes, correct!
Hi fraind I Like your jod
I needed blue berry seeds garminet
You can try this method to germinate the seeds.
IDK y..but there's no audio on this video
Hi Pinkie,
The audio is perfectly fine and working from my end and for other listeners as well.
I think there might be a technical issue from your side. Check if you aren't on mute or your device is not causing the issue.
Try putting the seeds near a South Pole magnetic field 😉 What also seems to work well is putting the seeds in to structured water.
There's no such thing as structured water. It's a marketing scam.
🙋🏾♂️is that regular peroxide so that’s like a cap full to a cup of water
Can you reframe your questions?
So to summarize what you did: You soaked the seeds directly in a 0.3% hydrogen peroxide solution until you see the radicle?
Yes. And then transferred it to tissue culture cups containing TC media.
@@PlantCellTechnology Actually, I grow hydroponically, specifically kratky. The media remains to be just fertilizer and water. Once I have a root, I'm good. I have this test running now actually with three variables: 0.3% peroxide alone and with scarification, and just water all on a 83F heating mat in open light.
@@golfshoe9321 Oh! sounds amazing. Let us know how did your experiment go.
@@PlantCellTechnology Long beans typically take 4-6 days. With 0.3% and scratcing the surface at 83F, it took less than 18 hrs to form the radicle!
@@golfshoe9321 Thanks Marlon for sharing this info with us.
Can i germinate rhizome using this method?
We've tested it for seed germination only. Not sure about rhizome.
Can I buy some media from you?
You can purchase MS media from the PCT store. Use this link: www.plantcelltechnology.com/murashige-skoog-medium/
@@PlantCellTechnology thank you sir. Is this a rooting media? Isn't there a multiplication media as well?
@@thembrelectric This is to germinate seeds.
You say 1 part peroxide to 9 parts water, but in your newer video you said 1 part peroxide to 19 parts water.
czcams.com/video/ErMyL8ag6go/video.htmlsi=lbCZHVFGZ6yQhB5V&t=44 9 vs 19 is a very large discrepancy.
No, it's 19:1 parts only!
Not to germinate, but to sterilize. I know they need a fungus or media
What the heck
Better to use food grade Hydrogen Peroxide, doesn't contain stabilizers
That's one amazing piece of advice you have given Edward. Will definitely look into this one. Thanks!! :)
That is what I used today for my cyclamen seeds. Funny coincidence. I just have this type of H2O2 at home. I will let them soak for 24 hours. I think all of them are dead. But I will try.
Why when nature been doing ok for a millions of years Just more chemicals People will wake up ne day But like the heating planet We left it too long .
Its physical properties are very similar to those of water, except that it is denser. No one is forcing you to use H2O2, keep using water if you want. Some of us though like to try new things and discover new ways of germinating seeds.
Can I use this on blueberry seed??
We didn't give it a try on Blueberry seeds. But, maybe you can work with a few of them to see how it goes. It only requires H2O2, water, and sugar.