- 57
- 5 291 281
Applied Biological Materials - abm
Canada
Registrace 22. 11. 2014
Subscribe to our channel for educational videos covering essential topics in cell biology and life sciences. From practical "How-to" videos for those ready to get started in the lab to videos introducing basic principles of important new scientific techniques. Send us your requests by posting a comment!
Applied Biological Materials (abm) provides quality research reagents and custom services at incredible prices. We have an extensive product range to make abm your one-stop supplier for all your research needs: PCR, RT-PCR, qPCR, antibodies, siRNA, miRNA, CRISPR, stable cell lines, immortalized cell lines, growth factors, and various viral expression systems (adenovirus, lentivirus, AAV). Conducting research shouldn’t cost you a fortune! abm is here to help!
→ Our company website: www.abmgood.com/
→ Our FREE learning resources: info.abmgood.com/learning-resources
#SciComm #CellBiology
Applied Biological Materials (abm) provides quality research reagents and custom services at incredible prices. We have an extensive product range to make abm your one-stop supplier for all your research needs: PCR, RT-PCR, qPCR, antibodies, siRNA, miRNA, CRISPR, stable cell lines, immortalized cell lines, growth factors, and various viral expression systems (adenovirus, lentivirus, AAV). Conducting research shouldn’t cost you a fortune! abm is here to help!
→ Our company website: www.abmgood.com/
→ Our FREE learning resources: info.abmgood.com/learning-resources
#SciComm #CellBiology
Introduction to Cell Transfection: Part 2
Welcome to "Introduction to Cell Transfection: Part 2" video! Are you ready to dive deeper into the world of transfection? Let's explore what transfection is, its application, methods to perform transfection, and go over a quick guideline for your consideration when preparing for your experiments.
Here are some helpful time stamps for our Transfection Methods video:
0:17 Calcium Phosphate Transfection
0:42 Simple Guide in Protocol (Calcium Phosphate Transfection)
2:08 Advantages and Disadvantages (Calcium Phosphate Transfection)
2:54 Lipid - Mediated Transfection
3:30 Simple Guide in Protocol (Lipid - Mediated Transfection)
4:47 Advantages and Disadvantages (Lipid - Mediated Transfection)
5:36 Physical Transfection
6:16 Simple Guide in Protocol (Physical Transfection)
7:29 Advantages and Disadvantages (Physical Transfection)
8:34 Summary
8:58 Thank You!
Featured Product Links:
Susfectin™ (Cat. No. G4000): www.abmgood.com/susfectin-transfection-reagent.html
DNAfectin™ Plus (Cat. No.G2500): www.abmgood.com/dnafectin-plus-transfection-reagent-g2500.html
Connect with us on our social media pages for fun educational materials introducing both practical and theoretical concepts in cell biology/life sciences:
➜ Facebook: appliedbiological.materials/
➜ Twitter: abm_good
➜ LinkedIn: www.linkedin.com/company/applied-biological-material
➜ Instagram: abm_good
➜ Blog: info.abmgood.com/blog
Here are some helpful time stamps for our Transfection Methods video:
0:17 Calcium Phosphate Transfection
0:42 Simple Guide in Protocol (Calcium Phosphate Transfection)
2:08 Advantages and Disadvantages (Calcium Phosphate Transfection)
2:54 Lipid - Mediated Transfection
3:30 Simple Guide in Protocol (Lipid - Mediated Transfection)
4:47 Advantages and Disadvantages (Lipid - Mediated Transfection)
5:36 Physical Transfection
6:16 Simple Guide in Protocol (Physical Transfection)
7:29 Advantages and Disadvantages (Physical Transfection)
8:34 Summary
8:58 Thank You!
Featured Product Links:
Susfectin™ (Cat. No. G4000): www.abmgood.com/susfectin-transfection-reagent.html
DNAfectin™ Plus (Cat. No.G2500): www.abmgood.com/dnafectin-plus-transfection-reagent-g2500.html
Connect with us on our social media pages for fun educational materials introducing both practical and theoretical concepts in cell biology/life sciences:
➜ Facebook: appliedbiological.materials/
➜ Twitter: abm_good
➜ LinkedIn: www.linkedin.com/company/applied-biological-material
➜ Instagram: abm_good
➜ Blog: info.abmgood.com/blog
zhlédnutí: 1 343
Video
Introduction to Cell Transfection: Part 1
zhlédnutí 3,1KPřed 8 měsíci
Today we will be exploring the cell culture technique, Transfection. In this video, we will help you learn what transfection is, its application, ways to perform transfection, and go over a quick guideline for your consideration when preparing for your experiments! Here are some helpful time stamps for our Transfection video: 0:28 Primer Length 1:31 Applications 2:54 Consideration - What do you...
Primer Design: Important Considerations and Tips for Good Primer Design
zhlédnutí 19KPřed rokem
Running a PCR experiment and need to design primers? We all have been there. Primer design is essential for successful PCR, but where do you start? In this video, we’ll go through important considerations and tips for good primer design! Here are some helpful time stamps for our Primer Design video: 0:41 Primer Length 1:06 Considerations for Melting Temperature (Tm) and Annealing Temperature (T...
High-titer Adenovirus and Titration
zhlédnutí 1,6KPřed rokem
Recombinant Adenoviruses are wonderful tools used by the scientists for high efficiency, large gene sequence transfers into almost any cell type! In this video, we'll help you how to perform high-titer viral amplification with adherent or suspension HEK 293 cells, virus purification using iodixanol or Cesium chloride density gradient ultracentrifugation, and virus titration by end-point dilutio...
Cell Culture Contamination and Quality Control
zhlédnutí 15KPřed 2 lety
You're in the middle of preparing your cell cultures for an experiment but wait a minute - is that contamination? If so, who's responsible? Cell culture contamination is a pain but if it's not dealt with it can become an even bigger headache! In this video, we'll help you recognize the different calling cards left behind by the most notorious cell culture criminals: bacterial contamination, fun...
Adenovirus Production: How to Clone, Package, and Harvest Adenovirus for Your Seed Stocks
zhlédnutí 8KPřed 2 lety
Adenoviruses are non-integrating and can deliver large transgenes (up to 6 kb) to a broad range of host cells (both dividing and non-dividing) with high efficiency, providing excellent yields of recombinant protein. In this video, we'll explain how to package second generation adenoviruses and generate high titer seed stocks! Here are some helpful time stamps for our adenovirus packaging video:...
abmVacations+ Rewards Program
zhlédnutí 377KPřed 2 lety
Take a break from labwork - join our abmVacations Program and go on your dream vacation! How does the program work? • Earn points for every dollar spent on any Applied Biological Materials (abm) purchases of life sciences reagents or services on our online store: www.abmgood.com/ What can I get with my points? • Exchange your points for: →Trip for 2 to Las Vegas →Trip for 2 to Cancun →Trip for ...
Lentivirus Transduction Protocol: Infecting your target cells
zhlédnutí 40KPřed 3 lety
So you've packaged and harvested your lentiviruses and you're ready to infect your target cells! But, what lentivirus transduction protocol should you use? Depending on whether you're infecting adherent or suspension cells or difficult-to-transduce cell types, we have different infection recommendations for you! In this video, we'll explain: → How to perform a lentiviral transduction experiment...
Lentivirus Titration: How to Determine Viral Titer
zhlédnutí 37KPřed 3 lety
So, you've harvested your lentiviruses and are ready to infect your cells! Hold on a minute - do you know how many lentiviral particles you have? It's time to calculate your lentivirus titer! In this fourth video of our lentivirus series we'll show you various methods for determining viral titer. This video covers: • Functional vs. Physical Titer • How to determine functional titer: FACS, Colon...
The Basics of Lentivirus Production/Packaging: Protocol, Tips, and more!
zhlédnutí 64KPřed 4 lety
Want to package recombinant lentiviruses? What packaging cell line should you use? How do you achieve higher titers? How do you harvest your lentiviruses after they’ve been packaged? Lentiviruses are a key tool in today’s field of biology as they provide a reliable way to achieve stable over-expression of a gene in your cells of interest. However, many scientists are confused about how to produ...
Protocol Walkthrough: GenomeCoV19 Detection Kit (Cat. No. G628)
zhlédnutí 1,5KPřed 4 lety
For more information about the GenomeCoV19 Detection kit, please visit: ➜ www.abmgood.com/genomecov19-detection-kit.html abm's GenomeCoV19 Detection Kit (Cat. No. G628) is an RT-qPCR Rapid Detection Kit for detecting SARS-CoV-2 from naso/oropharyngeal swabs in only 2 hours. Kit features: ✔️Highly specific for the RdRP and N target markers recommended by WHO and US CDC ✔️Ready-to-use reaction ki...
Cell Immortalization: How to Immortalize Cells
zhlédnutí 52KPřed 4 lety
Don't let your cells die before the end of your project! In this video, learn how to develop an immortalized cell line from primary cells. Read our blog post for more information on cell line immortalization: ➜ info.abmgood.com/cell-immortalization Timestamps for topics covered in this video: [0:29] What are immortalized cells? [1:19] How do you generate immortalized cells? [3:47] Cell line qua...
Multiplicity of Infection (MOI): What is it and how do I calculate it?
zhlédnutí 63KPřed 4 lety
Multiplicity of Infection (MOI): What is it and how do I calculate it?
Gene Silencing Methods: CRISPR vs. TALENs vs. RNAi
zhlédnutí 173KPřed 4 lety
Gene Silencing Methods: CRISPR vs. TALENs vs. RNAi
Getting Started with Whole Genome Sequencing - #ResearchersAtWork Webinar Series
zhlédnutí 66KPřed 4 lety
Getting Started with Whole Genome Sequencing - #ResearchersAtWork Webinar Series
Conditional gene expression using the Cre Lox FLEx vector switch!
zhlédnutí 134KPřed 5 lety
Conditional gene expression using the Cre Lox FLEx vector switch!
5) CRISPR Cas9 - Screening and Validation Strategies
zhlédnutí 48KPřed 5 lety
5) CRISPR Cas9 - Screening and Validation Strategies
One-Step Multi-fragment Cloning with the Pro Ligation-free Cloning Kit
zhlédnutí 2,5KPřed 5 lety
One-Step Multi-fragment Cloning with the Pro Ligation-free Cloning Kit
Gene Silencing Methods: CRISPR vs TALENs vs. RNAi
zhlédnutí 43KPřed 6 lety
Gene Silencing Methods: CRISPR vs TALENs vs. RNAi
The Cumate Inducible Gene Expression System
zhlédnutí 14KPřed 6 lety
The Cumate Inducible Gene Expression System
How to perform a CRISPR Knockin Experiment
zhlédnutí 97KPřed 7 lety
How to perform a CRISPR Knockin Experiment
Create a sanctuary for scientific discovery
zhlédnutí 1,1KPřed 7 lety
Create a sanctuary for scientific discovery
How to perform a CRISPR Knockout Experiment
zhlédnutí 98KPřed 7 lety
How to perform a CRISPR Knockout Experiment
abm - Celebrating Innovation Through Science
zhlédnutí 3KPřed 7 lety
abm - Celebrating Innovation Through Science
The Basics of the Recombinant Lentivirus System
zhlédnutí 183KPřed 7 lety
The Basics of the Recombinant Lentivirus System
4) CRISPR Cas9 - Gene Regulation with dCas9
zhlédnutí 70KPřed 7 lety
4) CRISPR Cas9 - Gene Regulation with dCas9
How a mini CRISPR Cas9 system is revolutionizing gene therapy!
zhlédnutí 6KPřed 7 lety
How a mini CRISPR Cas9 system is revolutionizing gene therapy!
CRISPR Cas9 vs. Cpf1: 5 ways the Cpf1 nuclease outsnips Cas9
zhlédnutí 23KPřed 8 lety
CRISPR Cas9 vs. Cpf1: 5 ways the Cpf1 nuclease outsnips Cas9
5) Next Generation Sequencing (NGS) - Applications of Whole Genome Sequencing
zhlédnutí 52KPřed 8 lety
5) Next Generation Sequencing (NGS) - Applications of Whole Genome Sequencing
How to extract genomic DNA from Plants - Plant Ex-Amp PCR Kit
zhlédnutí 6KPřed 8 lety
How to extract genomic DNA from Plants - Plant Ex-Amp PCR Kit
Really short n sweet
tysm!!
Tell me bout genomic technique at least there will work...
🙏🏻
Humans hijacking a virus hijacking a virus hijacking a human cell. Wow
Thank you for the feedback! We'll try to adjust the speed for new videos!
Hi! What secondary annealing means?
hairpin and selfdimer are secoundry annealing
Very helpful! Greetings from Brazil.
We're glad to hear that!
Very good guide.
We're glad to hear that!
I have created arms PCR primer; might I perhaps email it for revision?
Yes of course! Please email your inquiry to technical@abmgood.com, and our tech team will look into it! :D
@@abmgood thank you
PAM is located opposite strand compared to the strand where guide binds.
Fantastic, brief and awesome video, I was trying to understand the Crispr A system, it was so nicely explained here. Thanks a lot!
We're glad that it helps!
❤❤❤
Such a cute little video with lots of information. Tell me what happens to the experiments? Where do these mature “cells” go? The CRISPR-Cas9 creations?
We are glad that it helps! We will cryopreservation the cells after screening and QC. Please see this video for more about CRISPR-Cas9! czcams.com/video/1aJxXWkE3Ek/video.html
Pq no hay elado n la cafeteria de la USJ? kobhñp noug b
great
Thanks!
I was wondering why you included the PGK-Puro within the AAVS homology arms? If the CMV promoter is constitutive then can't we just select based on red flourescence?
Also because this way our knock-in only contains the GOI. My understanding is that this is necessary because we wouldn't want to transfect other selectable markers if we were using this for something commercial, like in agriculture.
This is the best I have seen so far on RNA-seq... Thank you so much, sensei.
Glad it was helpful!
Thank you for the wonderful visual+explanation! 15:53 May I know does the fragmentation occur before cDNA synthesis or after? I look through the protocol from Illumina they fragmented the RNA before cDNA synthesis
❤
link error: 404 not found
Thank u❤
You're welcome 😊
Even though this video was 8 years ago, it still seems great. I love it. Thanks a lot
Excellent work. Thanks so much for sharing.
No problem! Thank you for watching:D
You left out the all important part everyone wants to know. The kit costs $360 USD.
I made cell immortalization part of my morning routine and was able to cut my coffee consumption almost in half!
Who, if anyone, monitors and regulates CRISPR usage? What's to stop this tech falling into the wrong hands?
Amazing video thanks.. please reduce the speed of audio.
The background music is a noise.
❤❤❤❤❤❤
Can this technique be used to generate knock-in mice?
What is the use of ColE1? does that the origin of replication for the replication of plasmid inside the mammalian cells?
A brilliant, clear and comprehensive video!
Thank you!
fantastic video
Hello, Can you answer this question that if non-engineered lentivirus don't have psi gene for packing then what gene they use to do that ?
Additionally the repression/activation protiens can be engineered to bind to an extention of the guide RNA's so that multiple reactions can take place simultainously. The dCas9 shown here would only be good for one reaction at a time, where all genes targets are activated or repressed but cant do both for different sets of gene targets. RNA binding components are able to do that though and is also an option.
Very well explained introduction to Adenoviruses as vectors.
Micromolar filter 😂
I taught a neighbor boy how to make an electroporator from an AM FM radio powered by 9 volt batteries.
that is great thank you!
What is meant by Tm of product?...what is meant by word product?????
melting temperature
THis is my first clear understanding to PCR Am very impressed Thanks a bunch
very explanatory, thank you
Thank you so much for clarifying such a hard topic in a simple language. It was very helpful.
Glad it was helpful!
Tq so much for this informative video
abm u r the goat for making these videos. thank u
Nice explanation. Is it safe to consider Infection unit in this video is same as PFU(plaque forming units)?
❤
Great explanation ❤
😊