The Basics of Lentivirus Production/Packaging: Protocol, Tips, and more!

Want to package recombinant lentiviruses? What packaging cell line should you use? How do you achieve higher titers? How do you harvest your lentiviruses after they’ve been packaged?
Lentiviruses are a key tool in today’s field of biology as they provide a reliable way to achieve stable over-expression of a gene in your cells of interest. However, many scientists are confused about how to produce these genetic engineering marvels in their own labs.
In this video, we'll outline the steps/protocol for packaging lentiviruses, including:
• Considerations before you start [1:02]
• Basic packaging protocol [01:33]
• Storing your lentiviruses [4:19]
• Infection test experiment [4:56]
• Additional tips and tricks for lentivirus production
After watching this video, you’ll be well on your way to becoming a lentivirus production champion!
[4:05] Note: An error in pronunciation. It is a 0.45 µm (micron) filter (not micromolar) that is recommended for use.
You can read more about how to package lentiviruses and get a hard copy of the protocol here:
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Background music credit: "Back To Portland" by TrackTribe

[@rayzhang928]

abm u r the goat for making these videos. thank u

[@zaimin5103]

I can hardly understand without Chinese explanation,but i enjoy watching it.

[@luisdiel2144]

Is there a paper containing the steps and theoretical background to this system one could use for citation and you can share?

[@shirasagi9390]

great video, clear and simple. has the ''how to use your lentivirus'' video been made yet ? I cannot find the link

[@abmgood]

Hello there,
Thanks for the comment! Unfortunately, the "How to use your lentivirus" video has been delayed but we are working hard to release it soon! The next video will be "How to titer your lentivirus". Please stay tuned! :)

[@shirasagi9390]

@@abmgood thank you so much for your reply ! I am looking forward to your next video :D

[@the_encephalonaire]

Please can you make a detailed video on zinc finger system procedure & practical steps.

[@guoliangzhang1088]

It's a good description. Thank you!

[@abmgood]

Hi Guoliang, thanks for watching and leaving a comment - glad you enjoyed our video! Please let us know if you have any questions and we'd be glad to help!

[@jsingh265]

Thanks

[@tanishakumari2958]

Nice

[@ceylinzeybek3874]

can you share a paper that use this protocol?

[@senthilkumarkalyanasundara4028]

Great

[@kaylacomplains247]

hi can you please tell me which medium have you used for the subculture? i have a project about this and i am confused :(

[@abmgood]

Hello Kayla,
Please contact us at [e-mail] with a bit more details about your project. We will be more than happy to help you! :)

[@ellenstemm8079]

Are these avaliable in Australia

[@abmgood]

Hello Ellen,
Our Australian distributor will be happy to assist you further: www.abmgood.com/distributors/

[@UnderpantsMan123]

Great but I think you meant 0.45micron or micrometer not micromolar.

[@abmgood]

Hello,
Thank you so much for making our video clearer!

[@sam2theammyk9]

Micromolar filter 😂

[@eugenemazimpaka1241]

Thanks

[@abmgood]

Thanks for watching! :)