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My pleasure 😊

Sorry,please read 0.5 second inplace of 0.8.

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Thank you

Awesome! Thank you!

Almost any graph plotting software should do. If you don’t have access to a good package, you can draw the graph by hand on a piece of paper and read off the EC50.

@@professorGpharm is excel accurate?

What i meant is that, do we use this technique when we already know that the ligand is specific to the receptor of interest so our objective is just measuring the affinity? Because i thought that this method is to prove that a new found/ new synthesized ligand is specific to a receptor or not? Sorry i don't know how to articulate my thoughts

I think you are asking about selectivity for different receptors, rather than specificity of binding. Binding assays only measure affinity with the receptor under study. To investigate selectivity for a receptor, you would repeat the assay with other receptors to measure the drug’s affinity there.

​@@professorGpharmthank you so much for the response, ❤so just to be sure i got it, we do binding assays after proving the selectivity right?

Thank you.

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These are big topics. Do you have a particular area in mind?

Thanks for the sub! Pleased you liked it.

I’ve been too busy working to make videos, but part retired now and have some free time. More to come!

@@professorGpharm I discovered your videos studying for a civil service exam about 4 years ago, right after I finished my PhD. Now I'm a federal professor here in Brazil, partly because of the quality of your videos. Thank you so much!

Welcome 😊

Wow, thanks

Thank you, much appreciated. I aim to make things clear, but never sure if Successful.

Glad it helped!

thank you

I am not familiar enough with all the available books to recommend one that would be good for beginners. Perhaps someone else seeing this comment might like to suggest one.

@@professorGpharm thanks

I've had a bit more free time lately to make videos. Will try to post more regularly in future, but it depends on what else I have to get done.

@@professorGpharm best of luck 🤞

Considering the amount of time since your comment I assume you have solved this issue :). But for anyone reading, negative numbers for log(concentration) is fine. It just means you are using concentration values less than 1, since crudely speaking logarithm to base 10 tells you where the number “starts” relative to the decimal point. If you are uncomfortable with the negative numbers you can get the numbers to be all positive if you change the units for your concentration. For example, in this example you say you have concentrations of 0.01 and 100 - let’s say your units are in microMolar. Without changing then these values would become -2 and 2 after taking the log (base 10). You can then change these values to both be in the nM scale by multiplying by 1000. So you’d get 10nM and 100000nM. Now if you take the log of these values you would get 2 and 5 respectively. What is more important is to make sure you are consistent with the units, and know how to successfully convert back from the log(concentration) value to the actual concentration.