I couldn't be me more thankful to you for these videos. Amazing. I have been studying a paper for a PhD interview and I couldn't understand a thing. But thanks to your videos, now I do. Thank you again. Keep up the good Work, Please.
Regarding the step 3 of transgenesis, the procedure through which the transgene is introduced is called TRANSFECTION. Transformation is done autonomously by micro-organisms ( as demonstrated by the Griffith experiment). Nice video and well done.
Transgenic insertion of a plasmid containing the mouse gene of interest may be performed to generate an overexpression model, but this has an unknown number of gene insertions. For a single-copy insertion overexpression model, we can perform PiggyBac-on-BAC transgenesis.
in the 3rd step of transgenesis you insert gene of intrest with vector (plasmid) , why cant we just directly inject naked gene into the male pronucleus
I couldn't be me more thankful to you for these videos. Amazing. I have been studying a paper for a PhD interview and I couldn't understand a thing. But thanks to your videos, now I do. Thank you again. Keep up the good Work, Please.
Great Explanation! Thanks for the effort!
how is proteins like primase and polymerase are created/?
Very well made Explainty! It's a shame your Channel and Videos don't get as much attention as they deserve.
Keep going, your videos are great.
Regarding the step 3 of transgenesis, the procedure through which the transgene is introduced is called TRANSFECTION. Transformation is done autonomously by micro-organisms ( as demonstrated by the Griffith experiment).
Nice video and well done.
Did you test it on yourself ??
Thanku.for giving such a valuable content....👍
Very Informative and brief. Can you please give details to prepare Foster mother by inducing progestron harmon. Timings, dose and other details.
Thank you, for your nice explanation!
@@Cyagen please don't I will have to report you
Thank you❤️😊
From Sri Lanka 🇱🇰😍
Thank u so much for explaining and the animation
Are you volunteering?? It's just an injection 🤣🤣🤣🤣
@@zabranjenakojasko6833 IDK what u mean they rlly explained it good
it sounds like a complicated selective breeding...
plasmids occur in prokaryotes. can recombinant plasmids be inserted into mice?
Yes
Could i use this technique to over-express a gene already in a mouse? If not, what other methods can i use for this?
someone over-expressed my balls genes; they are HUGE
Transgenic insertion of a plasmid containing the mouse gene of interest may be performed to generate an overexpression model, but this has an unknown number of gene insertions. For a single-copy insertion overexpression model, we can perform PiggyBac-on-BAC transgenesis.
in the 3rd step of transgenesis you insert gene of intrest with vector (plasmid) , why cant we just directly inject naked gene into the male pronucleus
Me: Okay so let's search to find the answer it's 21st century after all
The video: the exact mechanism has not been determined
Me: 👁️👄👁️
i like this explanation but the “youtuber” accent is rlly annoying lol