Analysis of 16S data using DADA2 presented by Damien Cabral For more info: www.brown.edu/academics/compu... Tuesday, November 7th 2017 Brown University
R can't find the function - it could be you need to reload your library(dada2) line, also double-check that you didn't misspell filtFs (or whatever your named list of files is). Are you using a mac or a windows?
At 41:09, where you are creating variable named "map", you have used a file "Tutorial_Map.txt"... Can you tell or provide any link from where we can get this file ?
I am running in a problem with filter and trim command. I am not getting any results after running this command. It says check your parameters for filtering.no gz file written. Kindly help me out for this issue
maybe mostly due to intensification of over-clustering (clusters grow in size due to the extra bridge amplification reaction performed for reversing the DNA fragments and approximate each other, thus, lowering the purity of sequencing signals of neighboring fragments).
Such a helpful lecture with wonderfully clear explanations, thank you!
Your presentation is extremely clear. Thank you so much!
These are really good explanatory videos from the workshop. I would like to see the other videos.
Thank you for this! Your video is great.
AWESOME! THANK YOU!
so good! It helped me a lot
great introduction to DADA2. thanks for that
Amazing video! Is the Phyloseq video available?
good explanation! thanks alot!
Why I couldn't run error rate estimation function? The error was coming out as below:
> errF
R can't find the function - it could be you need to reload your library(dada2) line, also double-check that you didn't misspell filtFs (or whatever your named list of files is). Are you using a mac or a windows?
can you put the code used in this tutorial in a comment or in the description?
if i got Identified 118496 bimeras out of 125600 input sequences. during running command seqtab.nochim
At 41:09, where you are creating variable named "map", you have used a file "Tutorial_Map.txt"... Can you tell or provide any link from where we can get this file ?
I'm having trouble installing the 'dada2' package in the latest version of R. Could you suggest a compatible version of the R package?
dereplication is not part of DADA2, is it? What did you use?
I am running in a problem with filter and trim command. I am not getting any results after running this command. It says check your parameters for filtering.no gz file written. Kindly help me out for this issue
where do we find the Tutorial_Map file?
Did you find this file... I'm also unable to get it
Why always quality of reverse reads decreases more than forward read
maybe mostly due to intensification of over-clustering (clusters grow in size due to the extra bridge amplification reaction performed for reversing the DNA fragments and approximate each other, thus, lowering the purity of sequencing signals of neighboring fragments).