Every one of your videos is so impactful. I literally think I've made a change to my daily life every time I watched one of your videos recently. No other channel has had that kind of impact.
I once had a dream where I met an old woman who looked very young. I asked her her secret and she pointed to a table full of berries and chocolate. I have concluded my subconscious was saying flavonoids!
Chamomile Tea is my favorite. The new fancy one is astaxanthin, which I use along with other NR and NAD boosters. Asta has oxygen-containing components [ketone (C=O)]. "The AI singled out three compounds that have an 80 per cent chance of increasing the lifespan of elegans: *Flavonoids (anti-oxidant pigments found in plants that promote cardiovascular health;) [Number one was Diosmin - Citrus] , *Fatty acids (such as omega 3) [Number one was Gamolenic acid - Hemp, oat, barley], and *Organooxygens (compounds that contain carbon to oxygen bonds, such as milk & alcohol)." Interesting note regarding the old person blood test, and liver issues. "Even without any specific diseases, lifelong stress on vital organs is particularly high. Normalized entropy stress (with rest of the body equal to 1) is 37 for the heart, 34 for the kidneys, 17 for the brain, and 15 for the liver (Annamalai and Silva 2012)." So Heart health is always first; not surprising. Kidneys is number two.
Thank you for the warning! Elderberry bushes grew wild where I grew up, and people made pies out of the berries. I didn't realize raw ones were toxic. But I see from this website that they are. www.elderberryedgefarm.com/pages/are-elderberries-toxic-a-personal-experience
You should post about how NAD increases in the blood is useless without upregulating ENAMPT, so it can be absorbed into tissue and muscle. Levels of H2S in the body don't just act as a mitochondrial anti oxidant, it also upregulates ENAMPT. If you want to utilize NAD you need H2S. P.S Beta-Lapachone for increasing NAD levels.
How important is ENAMPT vs NAMPT. Exercise appears to give a dramatic boost in NAMPT, but not ENAMPT (at least in the following study)."Moreover, aerobic exercise training increased NAMPT abundance 12% and 28% in young and older individuals, respectively, whereas resistance exercise training increased NAMPT abundance 25% and 30% in young and in older individuals, respectively." See Aerobic and resistance exercise training reverses age‐dependent decline in NAD+ salvage capacity in human skeletal muscle,Physiol Rep. 2019 Jun; 7(12): e14139. I did see that fasting seems to increase ENAMPT in mice.
There is a sorgum festival in West Liberty Kentucky, from which I've acquired several bottles, but they tend to languish in the back of the pantry. Thank you for the new motivation to incorporate it into my diet.
Thanks for the video Michael! I was curious and I crossed all the database of the IC50 study and all the flavanoids per food to check other food sources that maybe could make it to the top10. They are really country specific like "Tasmanian peppers" or "Illawara plums". Let's see how we implement all those in our diet.
One additional comment I'd like to offer is that it might be very interesting to see if there are any synergistic effects of these different molecules on cd38 concentrations. Do they all use the same mechanism to inhibit cd38 or can they be combined to yield non-linear advantages?
Your videos are awesome! I always enjoy them and proceed to go down rabbit holes/ searching for studies concerning, for instance, enhancing bio availability. Then I'm lost for an hour, lol.
What about aronia-berries instead of blackberries? In general I don't think that you can compare the effects in vitro to organism. There are publications showing that isololated antioxidants in high doses can have side effects and trigger cancer. The best thing you can do is eating healthy with a lot of vegatables and herbs and not to take to much isolated supplements...
wowie, if apigenin half life is that high, frequent parsley consumption would build up, and you would eventually have massive cd38 inhibition. Thankfully not too worried as Tabbouleh seems to be a salad made mostly of parsley eaten in middle east, and haven't heard of long term issue from that massive parsley consumption.
@@conqueragingordietrying1797 'Luteolin has a half-life of less than five hours, which means it's necessary to take it multiple times a day to maintain therapeutic levels in systemic circulation." edu.emersonecologics.com/2018/05/17/luteolin-a-flavonoid-for-neuroprotection-and-immune-modulation/ "Therefore, urinary excretion and clearance of apigenin are quick, with the excretion half-life estimated to be approximately 12 hours." www.hindawi.com/journals/bmri/2019/7010467/ I did not read the studies or do any sort of comparison.
Thank you very much for that I recently tried an NAD booster (liposomal NAD along with TMG), and it had a strong effect... no biochem testing, but the macroscopic effect went into the wrong direction. I get very tired, and even kind of exhausted by it. Seems my body is not tht happy with it. So I will try the other route. elderberries needed in grams: 1mcM ~300mcgr, 6mcM ~2mg , x70 kg == 140 mg from fresh fruit, which contain 700mg/100gr, meaning that one would need 20g fresh, and max. 12g dried fruit. 500g dried around 20€ = 40 doses, which is cheaper than the pill. (and more doable than 8g dried parsley... at lest for me :) For blackberries 5.3 times more of course this assumes very good and rather quick absorption.
A real breakthrough would be to understand, why CD38 is increasing. In general: Why do we age at all? There are lots of thing increasing or decreasing. But why do these things happen?
One reason for the age-related increase for CD38 is because of LPS: czcams.com/video/NGrYzOKGBXA/video.html In terms of why we age, humans haven't lived long enough for us to have enough longevity genes.
@@conqueragingordietrying1797 Sorry. I was not clear. Why do these decline/increases happen at all during time? We move from one aspect to another, NAD to CD38 to LPS to IAP. But why do these things change at all over time? We always find new triggers, but not the underlying cause.
@@conqueragingordietrying1797 do you think it’s bc our body produces less stomach acid as we age. I would guess if there’s less stomach acid it would break down the gut lining and would make it harder to get the necessary nutrients for our body to balance IAP amounts.
@@MrKeith2005rt The acid in the stomach is supposed to stay in the stomach. If it leaks into the small intestine, and then the large intestine, that's a big problem. Nonetheless, that's not the most likely explanation for less IAP during aging. GI cells age, and that contributes to lower IAP expression into the gut. Also, poor diet impacts reduced IAP expression.
@@Avital4414 There's a wide variability for the apigenin content in Chamomile, see 12:57 of this video: czcams.com/video/iTYZN7xS3OY/video.html So it could be a good source of apigenin, but also not.
@@conqueragingordietrying1797 At least eating not cooked elderberries and high doses of dried parsley (used as an abortive in the past) could rise some concern.
Using your graph for Apigenin, it appears that about 50uM would be sufficient to minimize it. For Apigenin, there are 270g/M. So, 50uM is about 13mg (270 x 50 /1000=13). Does this mean that 13mg per day would be sufficient? If so the 50mg Apigenin supplement sold by Swanson would be more than enough to minimize CD38. Is that so?
@@conqueragingordietrying1797 Thank you, Michael. I find your videos quite informative. Do you have an idea as to what absorbtion % might be for Apigening and Myricetin? I actually Take 50mg Apigenin, 100mg Myricetin as supplements, but also consume plenty of Parsley, hoping to substantially inhibit CD38
Thanks@@zisoskatsiapis656. I haven't looked into absorption, that's going to take some digging, and will likely be in a future video, if there's enough data. My perspective is that eating a wide array of CD38-inhibitor containing foods is the best approach.
@@conqueragingordietrying1797 got organic black elderberry gummies for cheap(3.99 though newer bottles now at 6.99) in USA at burlington. But looking for the powdered organic fruit as that may be even cheaper. Fresh would be best probably, but my experience with fresh berries is that they spoil within days(at least strawberries do).
@@diamond_s It's unknown how much cyanidin is in the elderberry gummies. Fresh is definitely best, but from my experience, that's hard to find. Ha, I don't have issues with berry spoilage, I eat them relatively fast.
Thanks. Excellent and important information. If we eat a quality high fiber plant based diet, as you suggested before, we'll get quite a lot of these flavonoids plus lower gram negative bacteria and maintain gut barrier function which also lowers CD38. BTW, on a recent talk by Dr. James Kirkland, posted on the Healthy Aging at Tufts University youtube channel, he showed a list of substances which have senolytic activity. Among them were luteolin, quercetin, and fisetin, but they would be used episodically, maybe once a month for 2 or 3 days at fairly high doses to kill senescent cells.
I eat ~3 oz of blackberries every day, in addition to ~20g of parsley. The main point of the story is that the CD38 inhibition story is that it doesn't have to be all from dried parsley, as a wide variety of foods can inhibit it. Also, I'm planning on making tomato sauce more often, and adding dried Mexican oregano to it.
@@conqueragingordietrying1797 Another great video, thank you👍😁 I've been using 9 grams of dried parsley a day. I could easily add oregano as well. I learned that heat degrades apigenin so I add the dried parsley to the cooked food when it has cooled down. I wonder if dried oregano and it's flavone is heat sensitive? I will also eat 8oz of blackberries a day which is no problem 😁
@@stevefantastics6595 Thanks Steve. I haven't looked into heat stability or absorption of these flavonoids, but that's definitely on the list for potential future videos.
@@evab2132 Ha, that's old news, as I covered that in this video (czcams.com/video/5-2YoGctcCk/video.html). Part II of this series talks about other CD38 inhibitors that can increase NAD+
I prefer to get CD38 inhibitors from food, which would be expected to be the lowest-risk strategy for optimizing health. Once we get into artificial molecules, risk can only increase. There aren't any studies that have compared the long-term safety of 78c vs food for inhibiting CD38, though.
Every one of your videos is so impactful. I literally think I've made a change to my daily life every time I watched one of your videos recently. No other channel has had that kind of impact.
Thanks John, I do my best to have a unique perspective in this space!
Agreed
Radicchio is very common traditional dish in Italy, one of the oldest populations.
I once had a dream where I met an old woman who looked very young. I asked her her secret and she pointed to a table full of berries and chocolate. I have concluded my subconscious was saying flavonoids!
Chamomile Tea is my favorite. The new fancy one is astaxanthin, which I use along with other NR and NAD boosters. Asta has oxygen-containing components [ketone (C=O)].
"The AI singled out three compounds that have an 80 per cent chance of increasing the lifespan of elegans:
*Flavonoids (anti-oxidant pigments found in plants that promote cardiovascular health;) [Number one was Diosmin - Citrus] ,
*Fatty acids (such as omega 3) [Number one was Gamolenic acid - Hemp, oat, barley], and
*Organooxygens (compounds that contain carbon to oxygen bonds, such as milk & alcohol)."
Interesting note regarding the old person blood test, and liver issues. "Even without any specific diseases, lifelong stress on vital organs is particularly high. Normalized entropy stress (with rest of the body equal to 1) is 37 for the heart, 34 for the kidneys, 17 for the brain, and 15 for the liver (Annamalai and Silva 2012)."
So Heart health is always first; not surprising. Kidneys is number two.
No liver for sure
Elderberry syrup has a lot of interesting research on it in terms of immune system.
Kuromanin other names: Chrysanthemin / Cyanidin 3-glucoside / Cyanidin-3-O-glucoside / C3G
Word of caution on elderberries, they can be toxic. It’s recommended to cook them. See Dr Greger’s interview with his experience.
are you thinking of the red ones? I think the black ones are just fine.
Thank you for the warning! Elderberry bushes grew wild where I grew up, and people made pies out of the berries. I didn't realize raw ones were toxic. But I see from this website that they are. www.elderberryedgefarm.com/pages/are-elderberries-toxic-a-personal-experience
You should post about how NAD increases in the blood is useless without upregulating ENAMPT, so it can be absorbed into tissue and muscle.
Levels of H2S in the body don't just act as a mitochondrial anti oxidant, it also upregulates ENAMPT.
If you want to utilize NAD you need H2S.
P.S Beta-Lapachone for increasing NAD levels.
Ha, I've got a few NAMPT tabs open right now, I'm exploring whether to make a future video on it.
How important is ENAMPT vs NAMPT. Exercise appears to give a dramatic boost in NAMPT, but not ENAMPT (at least in the following study)."Moreover, aerobic exercise training increased NAMPT abundance 12% and 28% in young and older individuals, respectively, whereas resistance exercise training increased NAMPT abundance 25% and 30% in young and in older individuals, respectively." See Aerobic and resistance exercise training reverses age‐dependent decline in NAD+ salvage capacity in human skeletal muscle,Physiol Rep. 2019 Jun; 7(12): e14139. I did see that fasting seems to increase ENAMPT in mice.
There is a sorgum festival in West Liberty Kentucky, from which I've acquired several bottles, but they tend to languish in the back of the pantry. Thank you for the new motivation to incorporate it into my diet.
This makes sense why you eat a high amount of blackberries daily!
Brilliant. Great information. Thanks for sharing Michael.
Thanks for the video Michael! I was curious and I crossed all the database of the IC50 study and all the flavanoids per food to check other food sources that maybe could make it to the top10. They are really country specific like "Tasmanian peppers" or "Illawara plums". Let's see how we implement all those in our diet.
OMG nice vid!
One additional comment I'd like to offer is that it might be very interesting to see if there are any synergistic effects of these different molecules on cd38 concentrations. Do they all use the same mechanism to inhibit cd38 or can they be combined to yield non-linear advantages?
Thanks Michael ! Very useful info!
Your videos are awesome! I always enjoy them and proceed to go down rabbit holes/ searching for studies concerning, for instance, enhancing bio availability. Then I'm lost for an hour, lol.
Thanks Helen, and ha, I do that too-it's fun!
Thanks for sharing. I’ll be including these foods in my diet.
Truly excellent work, here 👏
Thanks Great Awakening!
What about aronia-berries instead of blackberries?
In general
I don't think that you can compare the effects in vitro to organism. There are publications showing that isololated antioxidants in high doses can have side effects and trigger cancer. The best thing you can do is eating healthy with a lot of vegatables and herbs and not to take to much isolated supplements...
Thank you Dr. I always enjoy your videos
Thanks Kim Pipi!
Time to add mexican oregano and celery seed to my meals.
Main benefit of apigenin vs luteolin is 90h half life. Side note:Also I have found it to help with my hypnogogic jerks.
Is there a published reference that compares the half-life of apigenin vs luteolin in vivo?
wowie, if apigenin half life is that high, frequent parsley consumption would build up, and you would eventually have massive cd38 inhibition. Thankfully not too worried as Tabbouleh seems to be a salad made mostly of parsley eaten in middle east, and haven't heard of long term issue from that massive parsley consumption.
@@conqueragingordietrying1797 'Luteolin has a half-life of less than five hours, which means it's necessary to take it multiple times a day to maintain therapeutic levels in systemic circulation."
edu.emersonecologics.com/2018/05/17/luteolin-a-flavonoid-for-neuroprotection-and-immune-modulation/
"Therefore, urinary excretion and clearance of apigenin are quick, with the excretion half-life estimated to be approximately 12 hours."
www.hindawi.com/journals/bmri/2019/7010467/
I did not read the studies or do any sort of comparison.
Thank you very much for that
I recently tried an NAD booster (liposomal NAD along with TMG), and it had a strong effect... no biochem testing, but the macroscopic effect went into the wrong direction. I get very tired, and even kind of exhausted by it. Seems my body is not tht happy with it.
So I will try the other route.
elderberries needed in grams: 1mcM ~300mcgr, 6mcM ~2mg , x70 kg == 140 mg from fresh fruit, which contain 700mg/100gr, meaning that one would need 20g fresh, and max. 12g dried fruit. 500g dried around 20€ = 40 doses, which is cheaper than the pill. (and more doable than 8g dried parsley... at lest for me :)
For blackberries 5.3 times more
of course this assumes very good and rather quick absorption.
Good video, thank you!
A real breakthrough would be to understand, why CD38 is increasing. In general: Why do we age at all? There are lots of thing increasing or decreasing. But why do these things happen?
One reason for the age-related increase for CD38 is because of LPS:
czcams.com/video/NGrYzOKGBXA/video.html
In terms of why we age, humans haven't lived long enough for us to have enough longevity genes.
@@conqueragingordietrying1797 Sorry. I was not clear. Why do these decline/increases happen at all during time? We move from one aspect to another, NAD to CD38 to LPS to IAP. But why do these things change at all over time? We always find new triggers, but not the underlying cause.
@@dirkh0 The underlying cause is that we haven't evolved to not age. Whether we can design tools to stop that in this lifetime, we'll see.
@@conqueragingordietrying1797 do you think it’s bc our body produces less stomach acid as we age. I would guess if there’s less stomach acid it would break down the gut lining and would make it harder to get the necessary nutrients for our body to balance IAP amounts.
@@MrKeith2005rt The acid in the stomach is supposed to stay in the stomach. If it leaks into the small intestine, and then the large intestine, that's a big problem. Nonetheless, that's not the most likely explanation for less IAP during aging. GI cells age, and that contributes to lower IAP expression into the gut. Also, poor diet impacts reduced IAP expression.
TLDR:
blackberries, black elderberries, oregano, radicchio, celery seeds.
Plus parsley, chamomile, onions, dill as apigenin and quercetin sources.
@@conqueragingordietrying1797 is it your sense that a few cups of chamomile tea daily is a robust (non-trivial) amount?
@@Avital4414 There's a wide variability for the apigenin content in Chamomile, see 12:57 of this video:
czcams.com/video/iTYZN7xS3OY/video.html
So it could be a good source of apigenin, but also not.
@@conqueragingordietrying1797 At least eating not cooked elderberries and high doses of dried parsley (used as an abortive in the past) could rise some concern.
@@riccardofabris1260 Right, which is why the lowest risk involves eating the whole foods...
They sell celery seeds online in 1 pound bags I’m thinking about adding this to some of my recipes maybe a teaspoon your thoughts?
My opinion is that adding some of all these foods may be an important strategy for fighting the age-related decline for NAD!
thanks
Quality!
Using your graph for Apigenin, it appears that about 50uM would be sufficient to minimize it. For Apigenin, there are 270g/M. So, 50uM is about 13mg (270 x 50 /1000=13). Does this mean that 13mg per day would be sufficient? If so the 50mg Apigenin supplement sold by Swanson would be more than enough to minimize CD38. Is that so?
That assumes 100% absorption, which may not be true. Also, that's why including many flavonoids that can inhibit CD38 is likely the best option.
@@conqueragingordietrying1797 Thank you, Michael. I find your videos quite informative.
Do you have an idea as to what absorbtion % might be for Apigening and Myricetin? I actually Take 50mg Apigenin, 100mg Myricetin as supplements, but also consume plenty of Parsley, hoping to substantially inhibit CD38
Thanks@@zisoskatsiapis656. I haven't looked into absorption, that's going to take some digging, and will likely be in a future video, if there's enough data. My perspective is that eating a wide array of CD38-inhibitor containing foods is the best approach.
Seeing elderberry powder online. Do you think that could be a good source (in terms of bioavailability)?
Ideally, fresh would be best. I haven't looked into bioavailability, that's likely a topic for a future video.
@@conqueragingordietrying1797 got organic black elderberry gummies for cheap(3.99 though newer bottles now at 6.99) in USA at burlington. But looking for the powdered organic fruit as that may be even cheaper. Fresh would be best probably, but my experience with fresh berries is that they spoil within days(at least strawberries do).
@@diamond_s It's unknown how much cyanidin is in the elderberry gummies. Fresh is definitely best, but from my experience, that's hard to find. Ha, I don't have issues with berry spoilage, I eat them relatively fast.
@@conqueragingordietrying1797 No no no, don't eat fresh elderberries, they're poisonous fresh and need to be cooked :)
Thanks. Excellent and important information. If we eat a quality high fiber plant based diet, as you suggested before, we'll get quite a lot of these flavonoids plus lower gram negative bacteria and maintain gut barrier function which also lowers CD38. BTW, on a recent talk by Dr. James Kirkland, posted on the Healthy Aging at Tufts University youtube channel, he showed a list of substances which have senolytic activity. Among them were luteolin, quercetin, and fisetin, but they would be used episodically, maybe once a month for 2 or 3 days at fairly high doses to kill senescent cells.
Could luteolin / quercetin / fisetin's mode of action be by reducing CD38 -> boosting immune system function -> clearing senescent cells?
Based on this data how are you changing your eating plan?
I eat ~3 oz of blackberries every day, in addition to ~20g of parsley. The main point of the story is that the CD38 inhibition story is that it doesn't have to be all from dried parsley, as a wide variety of foods can inhibit it. Also, I'm planning on making tomato sauce more often, and adding dried Mexican oregano to it.
@@conqueragingordietrying1797 Another great video, thank you👍😁
I've been using 9 grams of dried parsley a day. I could easily add oregano as well. I learned that heat degrades apigenin so I add the dried parsley to the cooked food when it has cooled down. I wonder if dried oregano and it's flavone is heat sensitive?
I will also eat 8oz of blackberries a day which is no problem 😁
@@stevefantastics6595 Thanks Steve. I haven't looked into heat stability or absorption of these flavonoids, but that's definitely on the list for potential future videos.
@@conqueragingordietrying1797 Here in Ecuador you can get 1 pound or more of blackberries for... $1. Amazing place.
@@conqueragingordietrying1797 I think I asked this before, but... do you measure your CD38 / NAD levels?
Treating plant molecules as medicine and not food has its place, but there are always side effects which in this vid are not explored?
Are most supplements of elderberries the black variant or something else?
Most likely black...
Is anything wrong to just take an Apigenin suplement?
Whole foods are the lowest risk approach, that's why I'd recommend that.
Dry parsley and chamomile tea has lots of apigenin. Tablespoon of dry parsley has 12x more, then fresh parsley.
@@evab2132 Ha, that's old news, as I covered that in this video (czcams.com/video/5-2YoGctcCk/video.html).
Part II of this series talks about other CD38 inhibitors that can increase NAD+
interstellarblends - peels / spices blends extract 200:1 is getting it for years.. people need to play catch up with interstellars!
Any thoughts on purchasing and self-administering 78c, which has been shown in studies to be an unrivaled cd38 blocker?
I prefer to get CD38 inhibitors from food, which would be expected to be the lowest-risk strategy for optimizing health. Once we get into artificial molecules, risk can only increase. There aren't any studies that have compared the long-term safety of 78c vs food for inhibiting CD38, though.
@@conqueragingordietrying1797 Thank you!
Never heard of these flavonoids before... Awesome!
I know you weigh all your food intake to correlate with your age bio markers- do you weigh your herbs and spices too?
Hey Amy, yep, I weigh everything except for about half a gram of ginger and turmeric powder that I occasionally put in my green tea.
I really think my Liposomal Quercetin capsules is going to be much more effective in raising NAD+
ok, ok...
is it in celery seeds and not celery ?
If you're taking about apigenin, then yes. See this database for that data:
www.ars.usda.gov/arsuserfiles/80400525/data/flav/flav_r03-1.pdf
would not the cost per mg be a consideration?
If that has value for you, go for it. For me, I prefer the lowest risk strategy, which is getting it from food.
What is cd38
CD38 degrades NAD
It's an inflamatory marker, an ugly mutha that inhibits NAD.
🙏
The only problem is that luteolin extract (not whole food form) is bad for neural stem cell differentiation and has a downside.
Got a published link that you can share?
💕
Black sorgham.is millet it's from africa
That's a LOT of oregano in your food ROFL.
Ha, that's a lot of oregano in the picture, I didn't say to put that much in food.
@@conqueragingordietrying1797 I meant a gram is a lot of oregano. A LOT. It's very light.