thank you in advance for your explanation. I don't have experience working GC-MS .after watching in the video I have a question . output of GC-Ms is chromatogram that shows abundance versus M/Z that molecular weight is calculated . how to calculate concentration of compound?
Hello Shiva, thank you for the question. I have uploaded a video on determining the concentration of compounds via the GCMS here: czcams.com/video/TGqoPLkOnA4/video.html Hope it answers your question. Thank you for watching this video.
Thanks so much for your explanation. I have a question for you. Is the mass spectrum sufficient to identify a compound or is it also necessary to have the IR, NMR and UV spectra? Thank you
Hi, It depends on the aim of the study as follows: 1. Shall you need to identify a new compound, mass spectrum, IR and NMR are needed. 2. Shall you need to confirm the presence of an already known compound, get the mass spectrum and verify it with the compound standard are recommended. Thank you for watching the video.
Hi there are multiple compounds detected per peak So which one will be the compound corresponding to the peak. Are all the compounds are said to be present in the single peak (whichever is detected).
Wslam sir! I have to compare compounds in plants of 4 different groups, is GCMS can interpret the different concentrations of the same compound in different groups of plant
Salam, GCMS can identify the volatile compounds in the plant. Here is the strategy: 1. Analyze the plant extracts via GCMS. Determine the detected compounds, qualitatively. 2. Based on the detected compounds, purchase the compound standards. 3. Analyze the standards via GCMS and set up a calibration curve for each standard. 4. Analyze the plant extracts. Then quantify each compound using the calibration curve.
Walaikum mussalam sir The library is showing three hits What we have to consider, only the first hit alone or all the hits Because it shows different compound name If it is same we can take that without doubting, hit is showing three different compounds for a particular peak Please help me in this
Hi Samsoon. In my case, if the extract is crude, I don't consider the hits as the correct result. Then, I submit the sample to another detection method to identify the compound. If the extract is the pure one, we could possibly accept the first hit; however, please bear in mind, the FTIR only identifies the functional group, not the actual chemical compound.
There are a few ways to confirm. 1. Confirm with the standard of the compounds. Run a standard of the compound, record and compare the mass spectra. 2. Based on the mass spectra of the compound, confirm the mass of ion fragments
@@shirwansany I mean when you click some peak many mass spectra is shown? I need to identify Z-7-dodecyl acetate ? how can i do that? Anyways thanks for your video.
Thanks Dr Shirwan
thank you in advance for your explanation. I don't have experience working GC-MS .after watching in the video I have a question . output of GC-Ms is chromatogram that shows abundance versus M/Z that molecular weight is calculated . how to calculate concentration of compound?
Hello Shiva, thank you for the question. I have uploaded a video on determining the concentration of compounds via the GCMS here: czcams.com/video/TGqoPLkOnA4/video.html
Hope it answers your question. Thank you for watching this video.
Thanks so much for your explanation. I have a question for you. Is the mass spectrum sufficient to identify a compound or is it also necessary to have the IR, NMR and UV spectra?
Thank you
Hi,
It depends on the aim of the study as follows:
1. Shall you need to identify a new compound, mass spectrum, IR and NMR are needed.
2. Shall you need to confirm the presence of an already known compound, get the mass spectrum and verify it with the compound standard are recommended.
Thank you for watching the video.
@@shirwansany8706 I thank you so much!
Can we identify compound on the basis of retention time ?
With GC/MS, you can identify a compound with (2) Mass spectra of the compound and (2) retention time.
Hi there are multiple compounds detected per peak So which one will be the compound corresponding to the peak. Are all the compounds are said to be present in the single peak (whichever is detected).
hello did you get the ans for this question???? i am also confused
I have the same question as well.
@@rahanjdas5572 nope
@@scientiainquisitor5391 thanks for letting me know
I have a question that how can we get this gc ms library???? and which software are you using??? Thank you in advance.
Wslam sir! I have to compare compounds in plants of 4 different groups, is GCMS can interpret the different concentrations of the same compound in different groups of plant
Salam, GCMS can identify the volatile compounds in the plant. Here is the strategy:
1. Analyze the plant extracts via GCMS. Determine the detected compounds, qualitatively.
2. Based on the detected compounds, purchase the compound standards.
3. Analyze the standards via GCMS and set up a calibration curve for each standard.
4. Analyze the plant extracts. Then quantify each compound using the calibration curve.
Walaikum mussalam sir
The library is showing three hits
What we have to consider, only the first hit alone or all the hits
Because it shows different compound name
If it is same we can take that without doubting, hit is showing three different compounds for a particular peak
Please help me in this
Hi Samsoon. In my case, if the extract is crude, I don't consider the hits as the correct result. Then, I submit the sample to another detection method to identify the compound.
If the extract is the pure one, we could possibly accept the first hit; however, please bear in mind, the FTIR only identifies the functional group, not the actual chemical compound.
Thanks!
Walaikum Salam :-)
Very nice
Very helpful
When you select some peak so many compounds were shown, how to conform the given peak is of respective compound
There are a few ways to confirm.
1. Confirm with the standard of the compounds. Run a standard of the compound, record and compare the mass spectra.
2. Based on the mass spectra of the compound, confirm the mass of ion fragments
@@shirwansany I mean when you click some peak many mass spectra is shown? I need to identify Z-7-dodecyl acetate ? how can i do that? Anyways thanks for your video.
Wow❤
How to calculate percentage by using giving data
You need to calibrate with known concentrations of compounds in question.
which is this application name where we can buy this app, pls share this link.
Hello, this is Enhanced Data Analysis software from Agilent, which came together when we purchased the GCMS.
Belajar lagi❤❤
Hi sir great video where can I get the software from I'm from south Africa thank you can you assist with software
Hello Franklin, I am using the Agilent OpenLab CDS Data Analysis software. It came with the HPLC instrument.
@@shirwansany8706 Agilent open lab software has using complex sir, i used above that software using friendly.
Can show how to open sample data firtst/
from Bangladesh
I need software plz share ur mail id
Hello, this is Enhanced Data Analysis software from Agilent, which came together when we purchased the GCMS.
Very helpful