Mass Spectrometry - Interpretation Made Easy!
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Instructor: Dave Carlson
Analytical Techniques Part 9 - How to Interpret Mass Spectrometry Data
This topic is no longer tested on the MCAT. Before watching this one, make sure to watch the Introduction to Mass Spectroscopy video : • Mass Spectrometry
You are such an amazing teacher! And great to know that you are better and reaching your dreams.
You just saved my lab report!!! THANK YOU SO MUCH 💜
Very, very, very HELPFUL!!! You're the best! After days watching many videos about the theory, machine, interpretation, just now with your video I got it! Thank you very much!!! S2
You are so good at explaining this! Maybe I’ll pass this organic practical after all.
Did you pass it I must know lol
@@voltage3208 I guess you will never know
C O V I D took them 😢
Haha 😂
Dear Mr, thanks a lot for sharing your knowledge
Great video! As someone hoping to be a Research Assistant someday, this really helped! :) Keep it up.
I hope you fulfilled your dream :)
amazing!!!! I love Dr. Carlson
Hey man, i just wann tell you that this video among other of your videos have helped me understand spectrometry a lot more, im currently an intern as a biomedical analist in a chemical analytic lab and was barely taught in this method, so thank you for the incredible explanation.
You look familiar
Nicely Explained. Thanks to the interpreter.
Very well explained!
Concised too
Thanks a lot !!!
Some lecturers are born with the talent of teaching :)) so he
So helpful for my job interview. Thanks.
Just in Chemistry II right now, but this video is sweeet! Can't wait to learn more about this stuff.
Screw Khan Academy. This man is wildly intelligent and down-to-earth. He makes learning for the MCAT so practical.
Why not both :( KA has so many useful MCAT vids
Screw free stuffs. Make one DH.
Singlehandedly helping me pass my analytical chemistry exam
Your english is easy to understand! Great class!
Great explanation, much appreciated 👍
Thank you so much!! Helped me a lot on organic chemistry!
Well explained. Thank you!
This helped so much. Thank you!
Thank you Sir for your wonderful explanation
Thanks for the interpretation 👍
Good video I was looking for something I’m creating a method for solvents and run into this and it’s a good video!
Well done, great explanation
amazingly explained
Quite explanatory, thanks
FFS Nobody ever explicitly said the part where you could get just an electron knocked off an nothing else and I don't know how I haven't realised it sooner but it helps so much.
The Spectrometry and the behavior of Elements can always relate on the ionic configuration, the way O2 can be increased for it to bind to other compounds and their respective strength. The Ionic configuration can be increased always, as long as it knows its base compound for it to have its molecular activity, either it repels or binds itself to other compound. Atomic and Subatomic group are also related to other elements aside from O2 and can always be adjusted in order to obtain the mass spectrum, volume and its measurements. The compounds that are co-valent to one another can guarantee at increase in strength of the compound atom or decrease in order to configure the strength for it to be in the chem.formulation related to chemical compounds and non-chem. ePLDT/PLDT/SME/SMC-Subsidiaries/Telecommunications and partner Industries, for your respective statistics to refer the basis of science integration as to behavior of the proteins and the atomic structure for it to perform according to its ionic configuration. Thank you Tierage 1 and 2 and Levels 1 and 2. SME-MCR. Date 2006 dated 2089 to date 2023. ******IMPORTANT******Thank you.
Thank you, thank you, thank you !!!!
You're welcome, welcome, welcome!
Thanks!
Thank you so much, Very easy to understand!!
very great lecture and method
Thank you!
Thank u . It is really helpful
Excellent 👍
It is very informative, Thank you Sir
dude saved my score on my course work
Thank you.
When I am analysing the MS data I have come across spectral count. What does that mean?
you are the best.
Misteeeer thank youuu 🤠
Great video! Please don't stop uploading like you did last time :)
+violinsheets Lots more coming!
Thank you
Thankss 4 this Informative video 👏👌
Wow!!! I like it
thanks very useful
thanks, dad
I meant, eg -OH-C=O may be cut into -C-OH, is it possible?
Can i get the video of GC-MS analysis and interpretation of a compound mixture.?
well explained
Thank yu so much
Good for A level chemistry thanks
thank sir
A low-resolution mass spectrum of the alkaloid vobtusine showed the molecular weight to be 718. This molecular weight is correct for the molecular formulas C43H50N4O6 and C42H46N4O7. A high-resolution mass spectrum provided a molecular weight of 718.3743. Which of the possible molecular formulas is the correct one for vobtusine?
Some one help me solve this
omg so cool!
helps a lot!:)
very good
Thanks so much. I sent the link to many that need to learn this. You are very clear in the explanation. Can we tell the absolute quantities of molecules in a solution? With a spiked in standard?
Yes, with the proper controls and methodology. Internal standards are used either as, or in conjunction with, system suitability tests to check the precision of the instrument before a run. Retention times and signal: noise ratios are checked that they fall within the proper ranges before a run.
Internal standards of a known concentration are spiked into samples that are extracted, as well as in a method blank to determine background noise that will then be subtracted from final values.
Peak area heights of endogenous analytes are plotted over the peak area of the internal standard, and this fraction is multiplied by the known concentration of the internal standard.
I would like to ask you a question in 2022 for the video uploaded in 2015 , is it possible to determine the concentration of our analyte of interest from the relative intensity of the spectrum??
I would like to respond since he is not here, no it is not possible. Hopefully you got a response before this year 2023
What if the fragment has z value of +2 or +3?
Great video, although magnetic field never accelerates any particle, that would be the electric field. Magnetic field is there to deflect the ions depending on their weight and charge.
yes does magnetic field only deflect?
So does the spectrometer send out photos and then increase the power of the magnetic field slowly until it detects the same photon it’s sending out?
could you please explain why the parent ion of glucose has less relative intensity
The mass spectrometer shoots electrons on the molecule and depending on the energy it is done with, it can split the molecule in smaller fragments or just steal one electron. The latter creates the parent-peak. My understanding is that the amount of energy with which the electron shoots at the molecule determines, if it get split or not.
With less energy, it get's less likely that the electron splits the molecule, so you can get a bigger peak for the parent ion. And with more energy it get's more likely to create fragments, so the parent ion's peak get's smaller.
I assume, that it is better to create more fragments than parent ions to be able to determine the structure of the molecule from the beginning.
Supper sir please put chem vedios related to basic chemisyry and after mphil level
What about 80 m/z? Is that the biggest fragment?
❤❤❤
Amazing lecture! I just have one question, why wasn't the mass of the parent ion 179? Shouldn't one hydrogen be missing from it? I'm really confused...
I am also confused about this question.
The electron beam doesn’t remove a hydrogen, it just removed an electron so the molecular mass is still the same.
@@ericcheong9150 ohhh you're right! Thank you so much
So if I point a mass spectrometer at myself will it tell me I need to stop eating donuts?
Could someone explain why we can trust the parent peak to be representative of the target molecule? What prevents the fragmented pieces of the molecule from recombining into a larger molecule that is not the same as the original. My only guess is that larger molecules tend to be less stable than smaller ones and with a shower of electrons would not be able to form or remain long enough to be detected before fragmenting again (since a larger molecule would have more mass, possibly allowing it to travel longer before reaching the detector, should the mass-charge ratio allow), I'm not sure about it though.
without electrons it is hard to form new bonds
all the ions are either cations or anions depending upon the mode of the machine but mostly cations. the ion extractor in the machine only extracts cations/anions to the analyzer. so chemical reaction between the same charge is not possible..
Great video!
I have a little question, the "z" value is taken as 1 in the calculation. Where does this definition come from? Is it because of the MS setting?
It is because most cations have a charge of 1
@@sacharaffaud1053 Thank you for your answer :)
What if all of the sample is fragmented? The largest fragments will appear at the right, but that will not be actual molecular weight of the compound!
I'm not an expert at all. But I feel like the probability is fairly low that 100% of the sample will be fragmented every time. It's probably likely that a few will slip through unfragmented, at least that's my interpretation.
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i dont see it
There’s hope for us all.
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Quick note, the furthest peak to the right isn't generally the M+ ion, but the M+1 or M+2 ion, depending on the typical isotopic presence. That ratio, M+ and M+x ions, will tell you how many carbons you have to determine the molecular formula.
Hello bro...can you please teach me this...if cant can you just give answers
"Methanol CH3OH was analysed in a moss spectrameter.Severeal Major PEAKS we're obtained at m/z values of 1,15,16,17 and 32.identify the ions responsbile for these PEAKS'
Can someone help me with this
This is Reeeeaaaallly late, but I'll answer it anyway. 1 is just a Hydrogen, 32 is the parent ion (mass of methanol), 15 is the methyl cation, and 17 is the OH. The 16 could possibly be the O, but I am not so sure.
OMG you're so cute when you smile! Had to watch the video twice lol
You heard the lady!
Yes, he's hot...and distracting because of that, LOL. Maybe that's why you had to watch it twice!
If he was my teacher I'd attend more classes o.o
Stuff like this can't be simple... 🤣😅
Hi I need help
Theoreticaly, but on practice I see somethimes some crazy picks ahahahha
Keenan Cornelius??
why all fragments charge +1 rather than +2? thank you for the great video.
Callie ham same question.
If you got the answer, tell me.
When u see 👀a super hot teacher...hard to concentrate isn't it?
Mass spec on the mcat? I don't think so
it might be
Maybe the concept, but not solving a mass spec problem
Some schools only require a 1 semester ochem class, so those kids are going to be screwed if they see this. Anyone who already took a 1 year ochem class should know this
Fucking boss
my nigga
What if your interpretation is wrong
lmfao I cant believe I use to know this crap
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what lol
too american for me
so long winded. just show us how to do it
Thank you