Factors Affecting Enzyme Activity - pH, Temperature, Competitive and Noncompetitive Inhibition

This Biology video tutorial discusses the factors that affect enzyme activity such as pH, temperature, substrate concentration, and the presence of inhibitors and activators. This video briefly explains the difference between competitive inhibition and noncompetitive inhibition.'
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how do you calculate specific activity when titration was done

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Have you done any video on enzyme inhibition?

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How can we get the amino acid residues of the active site in the catalysis

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If enzymes are reusable, why would the ligand ever be saturated after the reaction equals equilibrium? Wouldn't the new product be released and the previously-used enzymes would be available again?

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why doesn't the competitive inhibitor not do the same job as the substrate if they are exactly the same shape?

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