How to make Liquid Culture using Honey | Grow Mushrooms
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- Äas pĆidĂĄn 9. 09. 2024
- How to make Liquid Mycelium / Culture to grow Mushrooms at home. 3 ways of how to add mycelium to the mixture to innoculate a huge amount of grain spawn
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If you know you're going to store long-term, and want to purposefully stall out the mycelium, you can go without the air exchange hole. It's great for growing the mycelia quickly, but sometimes it grows too much. They won't die, they'll just grow very slowly, which is useful sometimes. đ
True! I have 6 month old spawn in my fridge which is pinning a lot... no fresh air would probably slowed them down till I need them
Thanks! Itâs hard to find videos that are informative and direct to the point these days and you nailed it!
Thank you:)
Tip for the application of the silicone. Use a wet cloth, or paper towel and press the silicone done. It will leave a nice and fine blob and will not leave a widespread mark of the silicone.
Or mineral spirits instead of water and can wipe clean
Wet finger. Just have a lil bowl ready. Aloe is even better
I spit on my fingers n wipe it. Who cares what way as long as works for u. But a great point nobody else makes is that by pressing it down/wiping it flat it makes guiding the needle thru the hole a doddle. Because it will be easier to see exactly where to guide it properly without hitting the metal lid(edges of the holes made)
Practical mycology !!! I saw his video made some great silicon caps
IMO honey gets too cloudy and hereâs my simple LC recipe.
1g light malt extract
1g peptone
600ml water
Dissolve and sterilize. Works great & very clear only mycelium shows.
Where do you get peptone?
@Victoriakarenka no thanks, I used to do that being an old Deadhead & all but gave it up. I appreciate you tho đđŒ
Great Tutorial
Thank you
I liked the way you shared the information. Great job đ
Good video, a time lapse of mycelium growing inside the jars would be interesting
Thank you!
I really want to see an example of how fully colonized LC looks in a jar. Plenty of videos on how to make LC, but no one ever seems to tell you how to tell when its ready to draw up into a syringe.
Nice, thanks for sharing!
đđ
I love How you just eating a chocolatebar in the middle of the video
Chocolate bar? Or Pickle!?
Or CooCumBurr
can you drink the culture like kombucha?
Thanks a lot â€â€â€
New sub, please make a time lapse of liquid culture in honey solution and keep posting frequently đđ»
thank you my mushroom friend.i have a question for you.i ate all the cucumbers now i want to make liquid culture from honey water.i have 10 cc syringe with spores dissolved in water.can i use your technique to inoculate?how would you go about it?thank you very much.
Thanks, nice videos, the couple I've watched.
Fucking love the way you say cucumber.
Thank you! So nice to see a vid using honey instead of corn syrup. How long does the LC stay good for once it's made and does storing it in the fridge extend shelf life?
I have a jar with healthy mycelium which is pinning on the Fluid for 3 years :D i Keep it closed as an Experiment
Oohh and it is stored at roomtemperature
@@HappyMushrooms dang, 3 years! I ask bc Ive heard the myc starts getting weaker after a few months unless you start a new batch and feed it more food. Maybe that's partly genetics too. Guess I'll have to try it out and see. Thanks again! đ€
â@@whitneylee33 Yeah, you need to give it a weekly stir. Basically, don't let it go stagnant. Not that it would completely, having continual growth occuring.
@@HappyMushrooms So you dont need mycelium to start with? you make it from scratch with this method?
Sour cucumbers, in the US we call them pickles đ
tnx!
The main secret is to use a cucumber jar đ
Can you inoculate bulk substrate with liquid culture?
Yes, absolutely.
Thank You for a good video. One question regarding storing temperature of the LC after injecting the mycelium. For a longer time it should be kept at 6-8 degrees C but what about the initial growth phase. Warmer for getting the mycelium to take of?
hm... good question. I just use it when it fresh (like 3-4 weeks old) or older mycelium directly from the fridge. Both seem to work fine
Nice video,. But one question sir I follow you r FORMULA and get it right but i use tissue from oyster and it product cocktails am confused why
Matic I follow you sirđ
I am gonna try this, but in the oven, saw someone do it when making spawn, i woder if you know anything about it? I feel like im gonna blow something up if i do it wrong. i don't have a preasure cooker.
I bought some growing kits, spore prints, spawns, liquid culture and psychedelic products from an online supplier and they are affordable and discreetly delivered
FROM
TRIPWITH_JEFF
Thanks for your effort.
You are welcome
Thanks for posting.
You're welcome
Thanks for the info.
Any time!
When I add honey to distilled water it instantly becomes cloudy even before pressure cooking it. Maybe it depends on the honey I use? Mine is organic honey.
Hm... I also used organic clear honey from a local beekeeper, but "normal" water. Maybe its the honey, but I have only tested this type of honey so its difficult to tell
@@HappyMushrooms most likely it is honey. My honey is not clear, it is like straight from the bee hive, not processed in any way.
Corn syrup, or dextrose should also work great and the fluid will remain crystal clear.
You really don't have to be concerned with the cloudiness. If it's sterilized then your good. Some or most of that will probably go away after several days and your growing a culture, not a clear glass of liquid so I personally wouldn't worry about it. You can also filter it through coffee filters before cooking and heat rid of some of that if you want.
Great video,well informed
Glad it was helpful!
Good morning đ»đ happy mushrooms đ I'm newbie about mushrooms đ please me ,big thanks đ God bless po â€ïž
Can natural maple siroup work? XD
Thank you for the video. When you do mushroom time lapse videos is it your mushrooms you film?
Hey yes all mushrooms are grown by myself
@@HappyMushrooms That's awesome đ. I started watching your videos because of the mushroom time lapse and then because of your photography tips and I just discovered your mushroom videos last week. Thank you for putting out great content!
how many teaspoons of honey for a half pint jar
Hey, great video!
Do I NEED to use runny honey or would I be also have good results with the "hard" honey?It should mix good too, right?
What is that black stuff in the beginning? Silicone of some sort? I can't hear, I have probably not heard the word before.
does it work work with magic mushrooms
Hi, Thanks for the video. Will it works with a spore srynge, injected to the liquid culture ?
Yes it does
Thanks !
Hay why not just microwave the jar until a rolling boil put on the lid on with no holes and flip upside down. Heat from the liquid will maintain 180f+ for more then 10min and this should be sufficient in killing spores and bacteria. Just a thought
@@brandoncoins8246 Would a metal lid be an issue in a microwave?
Itâs enough to pasteurize but not to sterilize. Sterilization requires 130 degrees. Water canât get hotter than 100 C at sea level because it will turn into gas. You need the pressure to raise the boiling point of water so that itâs still liquid at 130 C.
Do you incubate it at a certain temperature? And how long can you store it?
Thanks for the videos, i just stumbled upon your channel and find it very informative!
Hey, yes, I incubate at 21°C (70F) in a tent with temperature control. Great you like my videos:) Storing... dificult to answer: I have petri my mycelium in my fridge which work after 6-9 month. I also had grain spawn which started to grow mold after 3 month... Best way is to put it into fridge after 4 week, but I do not have so much space so I lose grain spawn from time to time if I wait too long (or it just starts to fruit)
Im currently enduring super hot temps where I live and my shop, where I keep the stuff has been getting above 80F -the liquid cultures I have really thrive well at these temps but I also know you can put them into a fridge to stall out the growth just make sure you stir or agitate somehow every couple days so you will be able to pull out syrige-fulls easier as needed.
@@codelicious6590 I'm not a professional I just love them.
If memory serves me. Some mycelium, mushrooms or microhorrzial species are more heat resistant. Where I'm at climate zone 6 rusula and oyster mushrooms thrive here. Also Ive seen amanita veroses and vaginata. In 90°f weather here
How can i make it with just a peice of the mushroom? Or do i need to buy a liquid cultured syringe
@Victoriakarenka ok
Danke fĂŒr das tolle Video, Jens :) kannst du mir sagen, warum man nicht einfach das Watte Loch als self-healing injection port nutzten kann? Die Watte geht doch genauso zusammen, wie das Silikon, oder sehe ich das falsch? LG aus Dresden, Thomas
Danke Thomas, ehrlich gesagt... habe ich mich an die Anleitung anderer gehalten. Ich muss hier raten: Die Watte könnte verunreinigt werden, ein Loch könnte entstehen (das könnte man natĂŒrlich wieder schlieĂen). Wenn die Watte dicht genug "gestopft" ist sollte es damit aber eigentlich auch klappen, zumindest fĂ€llt mir spontan nichts ein was sonst noch dagegen sprechen wĂŒrde. Bei Mehrfachverwendung ersetze ich die Watte, den Gummi/Silikon port nicht. LG aus Braunschweig
That very last jar shown on the last few seconds in the video,,,,,,, is that contaminated?? It looks different to the others colours and consistency. I have a jar that looks similar. Thats y i askđą
How much lc do you use for inoculate spawn?
5-10ml for a 4pound bag
1.5ml is more than enough
@@antoniosa932very true. As I actually use alot less that u. I simply just get a cluster of LC mycelium into syringe which on occasions its been as little as 0.2ml when using a 1ml syringe/18G needle end.
can this be done using water bath to sterilize. I do not have a pressure canner but would love to try this
I saw a lot of other growers just using boiling water.. but they boil for 10-20 hours instead of 45-90minuten. I think it will work, but I have not tested
@@HappyMushrooms There is also Tyndallization method. I've read different versions, but one I've used is to boil for 30 minutes, then let cool, and the next day reboil again 30min, for three days in a row. Some people think this is much too slow, but I think compared to the incubation period it's not bad at all. For a beginner, taking a little bit of time after work, for example, it's a good way to start without a pressure cooker.
Hold a pan lid down by finding a way to weigh it down safely is what i did on my first year, but still allowing for gas exchanges. And i boiled in pan for 3hrs. Ive done grain/LC/agar/manure this way. Never failed once i hit the 3hrs mark. But u gota not overfill pan and top up every 20-40ish mins. Itâs never been successful on 100% of sterilisation when less time is applied. Make sure all glassware is already sterile before u make the LC up coz i encounter contamination with old used food jars when i donât pre sterilise them prior to LC attempts
What is agar made of?
Literally agar. It's a kind of starch used in asian cooking. From some plant like a sugar beet I think.
You just contaminated it with your bare hand
can you add time code?
I bought pickles instead of cucumbers, please go slower.