It is not blue light that transfers it, but rather the energy of the excited state through resonance via the coupling of the transition dipole moments of the emitter (in this case the CFP) and the absorption of the acceptor (the YFP).
@Erick A - But the energy depends on the wavelength and frequency, so just calling it "blue light" already contains all that information - while still making it easy to understand.
@@velvetmagnetta3074 But it sounds like the CFP is actually emitting light from this explanation. The essence of FRET is that the donor does not emit light but rather transfers its energy via dipole-dipole coupling as was stated above.
@@mr.gallifreden1200 - Ok. I'm not trying to dispute the mechanism, just saying the color already implies the frequency which implies the energy. If they were to include all that extra detail, I feel like it would take away from the simplicity of the explanation. And anyone who already knows the mechanism wouldn't need this simple of an explanation anyway! It's like a starter pack or an overview. You can't pass any tests just by watching this video. But it can still whet someone's appetite enough to want to find out more about it. I just thought it was an odd gripe to have for this video since everything is represented properly, and that's just more, somewhat unnecessary, information.
Good video, but I missed the fact the F in FRET doesnt stand for fluorescence because fluorescence isnt crucial for the mechanism. It is also possible with bioluminescence for example. Additionally I have question: Do you know how I can determine what molecule is the D and A when the structures of both are are given?
It is not blue light that transfers it, but rather the energy of the excited state through resonance via the coupling of the transition dipole moments of the emitter (in this case the CFP) and the absorption of the acceptor (the YFP).
Thank you for pointing that out
@Erick A - But the energy depends on the wavelength and frequency, so just calling it "blue light" already contains all that information - while still making it easy to understand.
@@velvetmagnetta3074 But it sounds like the CFP is actually emitting light from this explanation. The essence of FRET is that the donor does not emit light but rather transfers its energy via dipole-dipole coupling as was stated above.
@@mr.gallifreden1200 - Ok. I'm not trying to dispute the mechanism, just saying the color already implies the frequency which implies the energy.
If they were to include all that extra detail, I feel like it would take away from the simplicity of the explanation. And anyone who already knows the mechanism wouldn't need this simple of an explanation anyway!
It's like a starter pack or an overview. You can't pass any tests just by watching this video. But it can still whet someone's appetite enough to want to find out more about it.
I just thought it was an odd gripe to have for this video since everything is represented properly, and that's just more, somewhat unnecessary, information.
Very good video.
Thanks for making and uploading
lovely explanation
omg appreciate you very much
Thanks!
so helpful thank you
thanks
Good video, but I missed the fact the F in FRET doesnt stand for fluorescence because fluorescence isnt crucial for the mechanism. It is also possible with bioluminescence for example. Additionally I have question: Do you know how I can determine what molecule is the D and A when the structures of both are are given?
could someone explain the exact difference between cross-excitaion and bleedthrough?
Well explained
Do the proteins have to be "attached" to exhibit FRET? What order of distance do typical proteins need to be to engage in this process?
No. This is the type of intetaction needing the overlap of orbitals. 5 nm - 10 nm are likely to be maximum distances of interaction
Tolles Viedeo Danke. Allerdings ist der Name Flouoreszenz -RET falsch
Danke! Was meinst du? Man kann Förster Resonanz Energie Transfer sagen oder mittlerweile auch Fluoreszenz...