Thanks a lot ma'am. I hope you would also consider demonstrating how is antimicrobial testing being done for extracts derived from plants or animals. Thank you
Thanks alot ma'am! I've been searching procedures to do a bacterial culture at home for months now! this helps alot on our research project Thank you po!
Gelatinase break down peptide bonds, which is present in protein-materials like the broth cube. But, agar is a carbohydrate, it is not a protein. How does the gelatinase produced by the bacteria degrade the culture medium? Is it because the broth cube/protein have been mixed well together with the agar solution that breaking peptide bonds also physically damage the agar? Thanks.
In my humble experience in laboratory, very few bacteria can degrade agar because it is cellulosic, made from algae extract. Gelatinase can work usually on gelatin made from animal extract which is usually 98-99% protein so you are right that it breaks down peptide bonds so it can solubilize gelatin.
Hi ma'am, thank you for this video. May I ask po what contaminating action did you do on the sample E (the one in the jar lid) for it to have that visible colonies compared to samples A to D? Thank you.
Hlo madam can we prepare culture media for bacteria using natural ingredients plzz reply I'm doing project on it and suggest some natural ingredients plzz 🙏
Yes you can. You can find something like seaweed as solidifying ingredients, boil meat like chicken and beef as source of protein. You may add natural extracts.
If used at home pwede na rin boiling water lang. If you have uv light you can use that too. In the lab, plastic petri dishes are disposables. You can buy it sterile then use it only once. It cannot withstand autoclaving.
The laboratory grade nutrient agar usually contains peptone, beef extract, and agar primarily as solidifying agent. In addition to these nutrients, some vitamins and some trace ingredients necessary for the growth of bacteria are also added. The peptone is the source of nitrogen or protein. The beef extract is the primary source of carbon which is essential for the formation of carbohydrates in the bacteria. It also contains other components like some vitamins, different trace minerals, organic compounds, and salts, which further enhance the growth of different organisms. Because I was only trying to make use of what is available at home, in the kitchen, I thought of using gelatin as it called (but really agar as they are made from 100% algae extract) to be the solidifying ingredient. I used beef cubes which is a replacement for two ingredients, the peptone and beef extract including different trace minerals, organic compounds, and salts for the purpose mentioned above. Protein is very important in culture media because it acts as a source of amino acids for the bacteria, necessary for gene expression.
Hello po Ma'am! We are tasked po kasi to take picture po after 2 days, 4 days and 7 days po tapos I may have pour the gelatin too early sa mold and as a result, the cover is slightly burry. Is it okay po to take picture without the lid off during those days? Or is it very dangerous to do so?
It is dangerous. You can devise a contained environment before opening it. Wipe the surface of the lid then put back before taking photo. Opening it without containment would mean contamination.
Ma'am gusto ko sana malaman kung pano gawin yung broth culture at home. I researched na need pa ng autoclave. Is it possible na may alternative way po to autoclave it? I need this po kasi to make my research feasible.
Broth culture medium can be done similarly minus the gelatin as solidifying agent. Alternatively, you can boil meat preferrably beef to get extract for better medium. You may use pressure cooker to replace autoclave.
@@jhonamiemabuhay1886 additional question maam hehe, is it ok if i'll put the whole bouillon beef cubes? im afraid baka mafail yung homemade agar ko po.. and what's the proper incubation po? thank u maam!!
@@baemax5769 hi. If you will use the whole cube it will darken na color of your medium. It will be difficult to see other colonies and maybe too much na ang trace elements like salt that may inhibit growth. Incubate at room temp not higher than 35 degrees para hindi magliquify
Maam may I ask po if we can use an Egg incubator to incubate the bacterial culture? the incubator also runs on 35-37C but not that stable po since bumababa and tumataas, can we use it po as a substitute incubator
Medyo mahirap po yan sa characterization ng colonies. You poured your culture medium too early. Kung sobrang mainit pa sya magiging wet talaga yan. Wait ka until medyo warm na lang ang medium before mo ipour sa plates.
thank you so much po I'm really using this for our home based research testing, but i have 1 question po, how do you dispose the agar plates properly??
@@jhonamiemabuhay1886 oooh thank you po, ito nalang po yung need ko isagot sa research adviser namin, tysm po naperfect ko po yung performance task ko.
Pwede naman buksan pero dapat sa laminar flow or Basta sterile environment para hindi macontaminate. Tapos dapat may gloves at mask kasi baka pathogenic Yan.. pwede naman iprolong ang incubation para lumaki colonies..
Good day po maam, bakit po pala bawal siya hawakan? so bawal mo po siya itransfer to any part of your body? And moreover po, when it comes to culturing good bacteria, same procedure lang po ba as shown in the video?
We are not sure kasi if what we are growing is bad or good bacteria. No way to tell. Mas okay mag-ingat. Same procedure lang although cannot separate bad from good bacteria kasi general culture medium sya...
It is better to use plain and unflavored coz flavoring might disrupt growth of microbes but if you want to try, why not. Then pls let me know the results. Microorganisms can grow just about anywhere...
I've tried before po using agar agar powder. Then overnight ko po sya nilagay sa ref, nagliquify pp agad sya 5 minutes after ilabas ref. Bakit po kaya?
@@suialekzandriamhai3328 try mo idouble concentration. Make sure natunaw ng husto ang powder. Kapag hindi sya natunaw, mabilis yan magliquify. Sa video, ang ginawa ko triple dose un...
Good day po, Ma'am. Can I ask if by using this way of culturing bacteria will it be possible to grow Propionibacterium Acne Bacteria? And if yes, how to do it po? I'm a SHS STEM student and I need the bacteria for my research po.
Isolating bacteria needs selective culture media. It is possible to grow bacteria from acne but to determine which one is your target is very difficult to do
Difficult to do it at home. You will need selective culture media for isolation. If allowede kayo gumamit ng lab, doable naman sya sa lab. You can use culture media like EMB agar, TSB or BHI
@@jhonamiemabuhay1886 okay po, Ma'am. Thank you so much for answering, this is a great help since I've been struggling with this po. I'll just edit my paper and do what you did. I'll also give credits to you po. Thank you so much ulit
As a hypothetical: What if you were stranded on an island and had to make penicillin from scratch …? How would you make your medium (if there were no Supermarkets)…?? THAT would be an interesting video….if you were rebuilding civilisation and had to make penicillin from scratch, could you?
Interesting. It would probably be possible considering that I know what to do, but it will take ages in the absence of materials and equipment. I would probably go for herbal plants as a source of antibiotic..
@@jhonamiemabuhay1886 Herbal salves/compresses would work for local infection - I've read that certain tribes used certain mosses and raw honey to dress wounds but as a treatment against something such as sepsis where the infection is throughout the body, would Herbal remedies be effect. Even Penicillin today has become ineffective against many infections. ...Also if herbal remedies WERE THAT effective you would think that they would already have been widely used during the many wars preceding the discovery of Penicillin in 1928 and it would have been redundant, right?
Hi po ma'am! isa akong farmer rice specialized nag reresearch ako ng mga microorganisms na makakatulong sa organic farming ko po. Nag aaral din ako kng ano ba talaga Ang kilangan ng bacteria and fungi to grow properly. At Ang isa Kong nalaman ay sa pamamagitan ng protein. Sana po meron akong maraming makuhang idea para makatulong sa pagsasaka.😊
@@jhonamiemabuhay1886 ma'am bakit ? Molasses Ang nilalagay na pagkain ng microbes po? Kc Ang sugar kapag subra Ang baba ng pH level nito. Meron po bang alternative para sa sugar or molasses? Ang ginagamit ko po kc ay kunting sugar lng mas marami Ang salt. Saging na hinog at kamote. Hnd po ba bumababa Ang pH ng lupa kapag molasses?
@@lazygardeneasylife2774 Okay naman ang molasses. Kelangan lang tama ang amount na maconsume sya ng microorganisms para walang excess. Marami sa microbes, esp fungi need ample amount of sugar for metabolism. They thrive better in slightly acidic environment. Magandang experiment yan kung gusto mong gawin. pwede mo imonitor ang pH para makuha mo tamong amount ng ammendments mo
Ok po salamat po ma'am 😁 karagdagan lang po na tanong. Nabubuhay po ba Ang fungi sa anaerobic environment? Para kasing kadalasan nasa topsoil lng Ang fungi po. Sana masagot🙏 Para magkaroon po ako ng better understanding 🙏🙏🙏❤️❤️❤️
@@lazygardeneasylife2774 meron din fungi sa sub soils or kahit sa medyo malalim pa na area. Meron pa rin amount ng oxygen. Pwede ang fungi sa anaerobic envi kung spores sila.
More similar content please! This is helpful! :)
Done watching! Love this! ❤️❤️❤️
👍😊
Cheap and easy to replicate, great video ma'am!
Thank you po ma'am for this amazing and informative experiment❣️
great help for our micro and parasitology this sem, thank you po ma'am!!!💗
Thank you for this simple and accessible agar plate tutorial it's a real life saver for my project 😭🙏
Happy to know that....
Thanks a lot ma'am. I hope you would also consider demonstrating how is antimicrobial testing being done for extracts derived from plants or animals. Thank you
Welcome. Yes, I will. Just finding time to do it. Maybe this sembreak...
@@jhonamiemabuhay1886 pleasant morning Ma'am🙏🏻💐 have you tried it? I hope I can privately contact you also
@@louisphilippes.sullivan9050 yes. a lot of times. yes you can look for me in fb
Hai ma'am. I already watched this many times with my classmate. Thank you po. It's very helpful to understand the lesson and the activity.
God bless
Glad it was helpful!
Thanks alot ma'am! I've been searching procedures to do a bacterial culture at home for months now! this helps alot on our research project Thank you po!
Can I ask po, can we use an egg incubator po as a substitute incubator? it has a temp of 35-37C din po kasi, thanks a lot po
Can we grow bacillus thuringiensis bacteria by this process?
Helped me a lot
Thank you so much Ma'am for this very helpful video. I'd like to ask though if using a colored gulaman is alright? Would the films still be visible?
I think yes. Colonies will still be visible. Pls try and let me know if you still get many colonies.
Mam which ingredients you add
That's great I would try that at home thanks
But I have a question is all kinds of gelatin can be used???????????????
Maybe yes but I suggest gelatin from seaweed. Many bacteria can liquify gelatin but not many if the origin is similar to agar..
Gelatinase break down peptide bonds, which is present in protein-materials like the broth cube. But, agar is a carbohydrate, it is not a protein. How does the gelatinase produced by the bacteria degrade the culture medium? Is it because the broth cube/protein have been mixed well together with the agar solution that breaking peptide bonds also physically damage the agar? Thanks.
In my humble experience in laboratory, very few bacteria can degrade agar because it is cellulosic, made from algae extract. Gelatinase can work usually on gelatin made from animal extract which is usually 98-99% protein so you are right that it breaks down peptide bonds so it can solubilize gelatin.
@@jhonamiemabuhay1886 Thank you for replying Ma'am!
Wow! Galing! Gandaaaa.. miss you Ma’am Jho! From Korea with love ❤️ 😘
miss you more. mukha ka ng koreano. pasalubong pag uwi mo
Mam can you say what was the bacteria that has grown from the dust particles from the table? Name the bacteria please?
I'm done na po ma'am. Thank you po!
Hi Maam, May I ask what did you use as gelatin? knox dissolves after 3 days.
I used the common snack gelatin. Not sure what you have in your area but check ingredients. It has to be from natural seaweed..m
Hi ma'am, thank you for this video. May I ask po what contaminating action did you do on the sample E (the one in the jar lid) for it to have that visible colonies compared to samples A to D? Thank you.
I just left it open for 5mins in the lab...
maam, is it required for the gulaman to have seawead extract or we can use the transparent one without the seaweed extract?
I fear that without seaweed extract it will easily be liquified by many bacteria...
Hello .mam, could this culture media will also work with bacillus thuringiensis and trichoderma? Am a farmer
It may work as temporary culture medium if you have pure culture. For trichoderma, add a bit of sugar. Will not be good for isolation...
You are using gelatine, made from animal collagen. Biologists normally use agar gel, made from seaweed. What is the difference?
The gelatin I used is made of seaweed.
Hlo madam can we prepare culture media for bacteria using natural ingredients plzz reply I'm doing project on it and suggest some natural ingredients plzz 🙏
Yes you can. You can find something like seaweed as solidifying ingredients, boil meat like chicken and beef as source of protein. You may add natural extracts.
Hello ma'am. How to sterilize plastic petri dish? Is it enough to use boiling water?
If used at home pwede na rin boiling water lang. If you have uv light you can use that too. In the lab, plastic petri dishes are disposables. You can buy it sterile then use it only once. It cannot withstand autoclaving.
Why do you need broth cubes po? What is the protein’s role in creating the culture medium?
The laboratory grade nutrient agar usually contains peptone, beef extract, and agar primarily as solidifying agent. In addition to these nutrients, some vitamins and some trace ingredients necessary for the growth of bacteria are also added. The peptone is the source of nitrogen or protein. The beef extract is the primary source of carbon which is essential for the formation of carbohydrates in the bacteria. It also contains other components like some vitamins, different trace minerals, organic compounds, and salts, which further enhance the growth of different organisms.
Because I was only trying to make use of what is available at home, in the kitchen, I thought of using gelatin as it called (but really agar as they are made from 100% algae extract) to be the solidifying ingredient. I used beef cubes which is a replacement for two ingredients, the peptone and beef extract including different trace minerals, organic compounds, and salts for the purpose mentioned above.
Protein is very important in culture media because it acts as a source of amino acids for the bacteria, necessary for gene expression.
@@jhonamiemabuhay1886 Thank you for replying Ma'am!
Hello po Ma'am! We are tasked po kasi to take picture po after 2 days, 4 days and 7 days po tapos I may have pour the gelatin too early sa mold and as a result, the cover is slightly burry. Is it okay po to take picture without the lid off during those days? Or is it very dangerous to do so?
It is dangerous. You can devise a contained environment before opening it. Wipe the surface of the lid then put back before taking photo. Opening it without containment would mean contamination.
Ma'am gusto ko sana malaman kung pano gawin yung broth culture at home. I researched na need pa ng autoclave. Is it possible na may alternative way po to autoclave it? I need this po kasi to make my research feasible.
Broth culture medium can be done similarly minus the gelatin as solidifying agent. Alternatively, you can boil meat preferrably beef to get extract for better medium. You may use pressure cooker to replace autoclave.
ma'am, is it ok if i'll use purified water instead of distilled and tap?
Distilled is the best but purified is better than tap. Yes you can use purified
@@jhonamiemabuhay1886 additional question maam hehe, is it ok if i'll put the whole bouillon beef cubes? im afraid baka mafail yung homemade agar ko po.. and what's the proper incubation po? thank u maam!!
@@baemax5769 hi. If you will use the whole cube it will darken na color of your medium. It will be difficult to see other colonies and maybe too much na ang trace elements like salt that may inhibit growth. Incubate at room temp not higher than 35 degrees para hindi magliquify
@@jhonamiemabuhay1886 omg, thank u so much maam!
Maam may I ask po if we can use an Egg incubator to incubate the bacterial culture? the incubator also runs on 35-37C but not that stable po since bumababa and tumataas, can we use it po as a substitute incubator
Yes, you can use it. Temp is just right. fluctuations is okay. better than ambient anyway...
Hi, is it okay po na medyo wet yung media? Due to the moist po after closing the container.
Medyo mahirap po yan sa characterization ng colonies. You poured your culture medium too early. Kung sobrang mainit pa sya magiging wet talaga yan. Wait ka until medyo warm na lang ang medium before mo ipour sa plates.
Good day ma'am, is it okay to use agar powder instead of gelatin?
yes agar powder is better
Thanks ma'am ❤️... Then needed pa po maglagay ng broth cube?
@@aprilremvlogs7951 yes. If you are using plain agar powder, need mo ang broth cube
@@jhonamiemabuhay1886 thank you ma'am ☺️
maam what water did you use is it distilled?
Only purified. Dringking water at home kasi nga trying to do this with home ingredients. But if you can use distilled, a lot better.
What is the alternate for broth cube ? please reply ?
boiled meat, any meat...
thank you so much po I'm really using this for our home based research testing, but i have 1 question po, how do you dispose the agar plates properly??
You cam simply put them in boiling water.
@@jhonamiemabuhay1886 oooh thank you po, ito nalang po yung need ko isagot sa research adviser namin, tysm po naperfect ko po yung performance task ko.
@@cyrustv3649 wow. Happy to hear that.
Ma'am Jo! Na kakamis ang lecture at Laboratory mo sa Microbiology na subject😁. Ang Galing mo pa din mag demo Ma'am 😁
Hehehe. Happy to hear that. Miss you all. Hope to see you again in the future. Amping.
Maam kapag po ba binuksan yung petri dish hindi na pwede isarado ulit tapos mag palaki ng colony of bacteria
Pwede naman buksan pero dapat sa laminar flow or Basta sterile environment para hindi macontaminate. Tapos dapat may gloves at mask kasi baka pathogenic Yan.. pwede naman iprolong ang incubation para lumaki colonies..
Ma'am how to culture staphylococcus aureus bacteria po using gelatine? Need help for research defense po
Hi. Do you have a source of culture of S. aureus or you need to isolate?
Mam plzzz name that all chemical that you have used for this.
Filtered or purified water, 1/4 beef broth cube, gelatin made from 100% seaweed.
@@jhonamiemabuhay1886 thanks mam
Can we skip broth cube ?I dont have it
You can.. instead you can boil a small piece of beef or chicken meat..
Yes you can.. instead you can boil a small piece of beef or chicken meat.. to serve as broth stock..
@@jhonamiemabuhay1886 thanks mam im going to do this for my college work....thank you so much 😊
Good day po maam, bakit po pala bawal siya hawakan? so bawal mo po siya itransfer to any part of your body? And moreover po, when it comes to culturing good bacteria, same procedure lang po ba as shown in the video?
We are not sure kasi if what we are growing is bad or good bacteria. No way to tell. Mas okay mag-ingat. Same procedure lang although cannot separate bad from good bacteria kasi general culture medium sya...
Is it okay to use flavored gelatin po? Thank you!
It is better to use plain and unflavored coz flavoring might disrupt growth of microbes but if you want to try, why not. Then pls let me know the results. Microorganisms can grow just about anywhere...
I've tried before po using agar agar powder. Then overnight ko po sya nilagay sa ref, nagliquify pp agad sya 5 minutes after ilabas ref. Bakit po kaya?
@@suialekzandriamhai3328 try mo idouble concentration. Make sure natunaw ng husto ang powder. Kapag hindi sya natunaw, mabilis yan magliquify. Sa video, ang ginawa ko triple dose un...
@@sasaadap4754 if sa lab, you can do autoclaving. Pag sa bahay, boiling will be enough.
is it neccessary to add the cubes
For me yes...
@@jhonamiemabuhay1886 kk
Maam, how about using blood agar? okay po ba yun?
Yes. Blood agar is selective and differential culture medium.
👍
♥️
Good morning po maam😊, have a nice day po.
Have a nice days rin...
What are the ingredients?
Gelatin made of seaweed, broth cubes and water
Good eve po ma'am can I ask where to buy Pseudomonas putida (bacteria)?
We dont have and I dont know where to buy. Sorry I cannot help you.. will try to ask around..
Ma'am student bio here😁 pa shoutout po ty po sa mga videos
Okay. Thanks for watching..
THANKS PO
Good day po, Ma'am. Can I ask if by using this way of culturing bacteria will it be possible to grow Propionibacterium Acne Bacteria? And if yes, how to do it po?
I'm a SHS STEM student and I need the bacteria for my research po.
Isolating bacteria needs selective culture media. It is possible to grow bacteria from acne but to determine which one is your target is very difficult to do
Where are you located? You can opt to buying Staphylococcus aureus from a lab near you
Hi maam! May i know po how did u know the microorganism's margin, color, elevation, texture, and shape po?
Hi. There is a guide for that.
Hi ma'am! Sorry for asking too much hehe. I just wanna know ma'am if there's condensates po, will I just invert it po?
@@baemax6905 if you were able to make your culture medium okay, you can incubate it upside down so moisture in the cover will be minimized.
@@jhonamiemabuhay1886 thank u Ma'am!
Thankyou ma'am it's very understandable and helpful po, if ever po can we use po a chicken cube instead of beef cube?
Yeah pwede naman...
@@jhonamiemabuhay1886 Thankyou for the response ma'am❤️
good day po ma'am. May I ask po how can I culture E.coli and staphylococcus aureus po? I need those po for my research paper
Difficult to do it at home. You will need selective culture media for isolation. If allowede kayo gumamit ng lab, doable naman sya sa lab. You can use culture media like EMB agar, TSB or BHI
@@jhonamiemabuhay1886 okay po, Ma'am. Thank you so much for answering, this is a great help since I've been struggling with this po. I'll just edit my paper and do what you did. I'll also give credits to you po. Thank you so much ulit
As a hypothetical: What if you were stranded on an island and had to make penicillin from scratch …?
How would you make your medium (if there were no Supermarkets)…??
THAT would be an interesting video….if you were rebuilding civilisation and had to make penicillin from scratch, could you?
Interesting. It would probably be possible considering that I know what to do, but it will take ages in the absence of materials and equipment. I would probably go for herbal plants as a source of antibiotic..
@@jhonamiemabuhay1886
Herbal salves/compresses would work for local infection - I've read that certain tribes used certain mosses and raw honey to dress wounds but as a treatment against something such as sepsis where the infection is throughout the body, would Herbal remedies be effect. Even Penicillin today has become ineffective against many infections.
...Also if herbal remedies WERE THAT effective you would think that they would already have been widely used during the many wars preceding the discovery of Penicillin in 1928 and it would have been redundant, right?
You should do more lab videos ma'am
Thanks
I am interested in bacteria for a reason lol
Watched
interested in micro biology in agriculture
permission to use your video madam for my science class, thank you
Ok
Hi po ma'am! isa akong farmer rice specialized nag reresearch ako ng mga microorganisms na makakatulong sa organic farming ko po. Nag aaral din ako kng ano ba talaga Ang kilangan ng bacteria and fungi to grow properly. At Ang isa Kong nalaman ay sa pamamagitan ng protein. Sana po meron akong maraming makuhang idea para makatulong sa pagsasaka.😊
You can ask me..
@@jhonamiemabuhay1886 ma'am bakit ? Molasses Ang nilalagay na pagkain ng microbes po? Kc Ang sugar kapag subra Ang baba ng pH level nito. Meron po bang alternative para sa sugar or molasses? Ang ginagamit ko po kc ay kunting sugar lng mas marami Ang salt. Saging na hinog at kamote.
Hnd po ba bumababa Ang pH ng lupa kapag molasses?
@@lazygardeneasylife2774 Okay naman ang molasses. Kelangan lang tama ang amount na maconsume sya ng microorganisms para walang excess. Marami sa microbes, esp fungi need ample amount of sugar for metabolism. They thrive better in slightly acidic environment. Magandang experiment yan kung gusto mong gawin. pwede mo imonitor ang pH para makuha mo tamong amount ng ammendments mo
Ok po salamat po ma'am 😁 karagdagan lang po na tanong.
Nabubuhay po ba Ang fungi sa anaerobic environment? Para kasing kadalasan nasa topsoil lng Ang fungi po. Sana masagot🙏
Para magkaroon po ako ng better understanding 🙏🙏🙏❤️❤️❤️
@@lazygardeneasylife2774 meron din fungi sa sub soils or kahit sa medyo malalim pa na area. Meron pa rin amount ng oxygen. Pwede ang fungi sa anaerobic envi kung spores sila.
💓