Get Exclusive step by step videos, 1on1's, private chat & much moređđŒ www.patreon.com/willymyco All social media and only social media đđŒ instagram.com/ttf_willy instagram.com/official_ttf_willy twitter.com/willy_myco Music By: D'rok The Menace TTF submitted and used photos S/O's instagram.com/mushlovedelivery instagram.com/texastoadstools
I use oven tek for my agar pours. Preheat to 250, crack it open and place a wire rack over the opening. Do your pours over the rack, good to go. Learned it from PGT. Thanks for the video Willy, good info for sure
I do mycology in the closet. No air current. I have pretty good results. With safe food handling practices. Bleach water spray bottle is my go to, sanitation for pennies, millet bird seed it the grain of choice you can get like a 30# bag for like $14, you can find damaged bags for like $3 at lowes and the depot stores.
I've had great success(10 agar transfers and 4 grain transfers with no contamination) by using this sab method and I am happy you posted about it because everyone thinks im crazy for not cutting arm holes. I started doing it a few months ago because I realized during a smoke test that when I put my hand into a sab(through holes) air is sucked in and it does the same thing when you exit. The trick is you have to have the opening flush with the edge of your kitchen counter so you arm movements do not push air into the counter and into the sab. You also cannot have any airflow pointed towards the opening. I also sanitize my hands and arms every time I leave the sab even if its for a second. If I am working more than 15 min then I spray lysol into the bin. You can spray even if there is stuff in there as long as none of your plates or jars are open. I actually think this is more effective than the traditional SAB with arm holes. The armholes are great if you do not leave the sab until you are done but if you have to exit for any reason then when you re-enter you push air from outside into the sab(from air displacement) directly over your samples. If you are disciplined with your movements (always reach from below or level with your samples) and with sanitizing using the open SAB is more effective.
Hello. How to sterilize a scalpel and an inoculation loop with an open fire in such a box? will fire create air movement or not? besides, it's flammable, because lysol contains alcohol, isn't it? Please help, I can't figure out this issue with the sterilization of a metal instruments.
@@user-ti3nq1pf1w I use a lab grade alcohol lamp too sterilize my tools. I do not keep the lamp in the SAB. I leave a few feet of open counter next to the SAB and sterilize it with alcohol and lysol before placing my lamp right next to the sab. Sterilize your instruments then while they are still red hot transition to working in the sab. Smooth but urgent movements are key to prevent contamination. Plan and practice everything you are going to do in the sab before you do it. This technique isn't perfect and there is still a risk of contam no matter how good you are. I have since upgraded my setup to a labrats laminar flow hood. Edit: let the lysol and alcohol settle/evaporate before you light anything outside the sab. There can be fumes inside the while you work but I always extinguish candle before I spray.
I use my 2nd wardrobe closet no clothes, storage products, no vents= no airflow- possible contam⊠I use the top shelf and got a few of the Boomer SHROOMER inflatable monotub. It took me a few weeks to find my groove getting my grains to not turn into contam. I barely go into my closet lab space, unless Iâm working, and I take a shower and turn ac off and spray my gloves, forearms, and upper torso with 70% iso. Also I use a plastic tote that melted 8 holes the diameter of a Campbell soups can heated up red hot, and carefully apply pressure into the tote for the holes to be melted in perfect circles no drill debris of plastic shavings. Iâm still learning but I finally got it down now. Next step Iâm making my own DIY Mini laminar flow hood all for under $80
Hah! I just saw this and Iâve been using my box exactly like this but I added an extra little safeguard. I added a cut piece of clear plastic tarp to cover the majority of the front part with a slit cut 1/2 way up so my arms can go inside freely.
So many people need to see things done to 'learn'. Reading about it is one thing.. seeing it with your explanation, I'm sure, helps a lot of people. Rock on!
instead of cutting the holes find a metal can about the size of your arms, heat it up on the stove and burn the holes through. leaves a much smoother finish and a perfect circle with less chance of cracking the tub.
I've only done it once right in front of the hot oven, advised by midwest. I got the kit from them. Have you heard of of oven method? After seeing this it does seem better than the oven method. I am going to do it my next go around. Anyway, you are a wealth of knowledge. I hope to be on the next zoom. headed over to P to see the next video now.
Yup I use a c-clap to keep rack secure. I learnt from Home Mycology. Taping a clear dry cleaning bag to small hepa fan works really nice as well. Makes huge sterile area to work
I saw a trick where someone put their oven on very low and had a wire trey reaching out over a slightly open oven. The theory was that the rising heat created a somewhat laminar flow upwards and he poured his agar that way with the dishes on the wire trey. I have a sab so I don't need to do that. But it sounds like a good idea for broke boy tech. Haha. It would be cool to see some comparative results with these different techniques to see if one has less contam rates then the other.
I've done that oven tech 4 times now making plates and slants and it works surprisingly well. I usually pour 4 cups of agar each time. I've been using the 2 ounce plastic condiment cups so I come out with quite a few. Probably about 50 but I usually do a fair amount of slants too which seem to have come out clean. Last pour I only have had one plastic cup with a little contamination so far. 25 cups I did of a separate syringe all came out dark brown everywhere the loop sneaked across the agar and it was fast. Second day I was throwing them away. The scary part was that I used the same loop for 4 different species one right after another. So we know that flame sterilizing does a excellent job.
Thanks! I like this, what I have done is take a similar sized bin, and wrapped shipping saran wrap around the top 2/3, leaving a wide hand port. I make it clean, but my bunsen into it for 10" and go. So far, so good.
"Cuts arms" omg that made me laugh. People with that little resolve might not make it in this hobby. The only reason I don't like a SAB is because I'm disabled from labor jobs due to my back. No worries I got a work from home job, as not working is the worst thing for anyone. The sab is horrible for posture and long sessions are not a viable option for me long term.
Another idea to add is Saran Wrap across the front. Leave a opening at the bottom. Easy to get your arms in to work and easy to see through. Keeps the work space a little cleaner
@happygolucky1100 Iâve used this cheap very effective method without l the hassle of buying very expensive equipment that you donât necessarily need but they are very good to have to eliminate altogether or reduce contamination
Lowes sells a small plastic greenhouse for 30 bucks. You put the frame together and slide the plastic shell over the top and cut 2 arm holes in it. Works great
Hello, just starting out and wondering if this method still works for you and is still your go to? I tried it and got decent results but I am new and those results could be from any contam point. I switched to a SAB and seems to be about 1-2 out of 10 grain jars contam. But they did not contam on matched AGAR samples I kept. I intend to go back to Your Idea but try all over now that I have more knowledge than before. Thats why I was asking what you still think a year later. Is it worth it to use the homemade flow hood? Obvious there is way better but I mean on par with your statement.
@@kylebrowning88 Still standing strong, I just replace the filter from time to time. I even used that setup to vent my chamber after misting to promote Evaporation. Works great.
I would recommend always giving the full term before using acronyms. This is pretty much the law of acronyms. Iâm new to this world and have no idea what an âSABâ is which was used at least 20xs in this video.
Working over a cracked oven with a rack also works nicely, shout out to Home Mycology. Have also taped open end of clear dry cleaning bag to HEPA fan. Turn on and cut arm holes in side, balloons out creating huge sterile area to work
Thanks PageG.....seems I saw that tek on another site. Yeah those "dry cleaner bags"...that cover shirts/coats/etc. are really a great idea. Thanks again Page. Almost forgot about it....
Also I gorilla taped and cut a small cross with a razor blade into two sandwich bags over the arm holes which were just cut bigger so i didn't cut myself. This keeps my feom cutting myself and keeps air from getting in when i put my arms in and out... And i have done the same thing, but with clear industrial strength Saran wrap over the top half but tbh the alcohol lamp just worked better for me as long as you work close. You just need heat to push it all away. Also you can build a laminar for about 80-100 bucks its just the right fan behind a HEPA and enclosed, im even going to add some UV lights
I never made a SAB. I took a tub and just turned it on its side and sprayed isopropyl alcohol on absolutely everything I touched and transferred to/from. Keep open flames away from that environment if you follow my lead. I've only had contam in a few agar cups. Transfers from agar to jars or jars to tubs have not had any contam. Not sure why only a few agar cups have contam and nothing else has. I suspect maybe syringes were contaminated?
Thank you for your videos! Itâs very interesting, you doing amazing job! Can you do video how to get 2nd and 3d flash for small commercial farm? I just started and canât find how to do in CZcams. Not sure if I have to soak each bag for 12 hours? Itâs a lot of work, is there is a way to do without soak in them and get 2-3 flashes?
Funny how no one in the community seems to appreciate the open flame technique, which is btw the standard in academic microbiology. Typicaly it would require a bunsen burner and a source of gas, but I work with fondue fuel and the lil burner that comes along and i get pretty decent results, working in the sterility cone. I'll admit I'm using PastyWhite's no pour agar technique though.
I was using an inoculation loop to start 4 different species on agar. After about 3 days I checked them and one species I had about 25 agar cups of was growing dark brown everywhere I streaked the agar plate with the inoculation loop. No white mycelium, just dark brown. The other 3 species have come out really clean and healthy. The ones that came out brown and contaminated where the first ones I did. Did all this in the same SAB Box, same batch of agar, with the same inoculation loop. If I didn't flame sterilize the scalpel, that would happened to everything. I only flame sterilized between each species. I really think the syringe is contaminated but I made more agar cups and put the spore solution on the agar directly from the syringe just to reduce as much contact as possible just to see if it happens again. Also flame sterilized the needle so I can hopefully narrow down what caused such an aggressive contamination. It took over 80 percent of the agar surface in just 2 days. It will be interesting to see if it happens the second time.
Do you still do this and what are contam rates like? Iâve been thinking about just working close to my alcohol lamp but not a lot of info out there on this.
You can make a flow hood for 40-100$. Only way to go. Either box fan with home filter taped to it. Or an eBay mounto hepa filter with an old squirrel cage and build a box. Hot glue works to seal shotty wood work đđŸgo from 5-10% contam to 0. I put it in an ice shanty to recirculate clean air
This is exactly what I did a week ago on a spawn to spawn transfer! Except I was also running 2 UVC w/ ozone lights inside the box. Iâve killed contamination with these lights. A must have!!
Please be careful with uv. My homie got wicked face/neck sunburn and couldnât open his eyes for a day. He didnât know it was on under a table, was out of sight. Had a remote and usually turned off hour before entering. He was in the room just over half hour and messed him up bad. Just be safe with them
I remember you on a diff channel im sure⊠i remember yr vids being super detailed and good a back in the day . Now energy prices are bs worldwide recn mushies would be good to get goin
This guy is not a good resource for mycology. You are better off with channels like Stipe & Cap, the Trusted Cultivator and sites such as the Shroomery.
im still figuring stuff out, the kit i have had me innoculate jars in front of my oven at full blast with the door down and doing it right on the door it self or holding in front of it at least. does this not work for things like agar and syringe and prints i take it? just curious, as it would be the most convenient i feel.
Carbon won't work for this. It does chemical filtration, removes molecular sized contaminants from the air. You need mechanical filtration, like a HEPA filter, to remove the larger bacteria/fungus spores from the air.
So i got a question. So say i get some plates and they dont germinate because the agar was too dry when you inoculate what should you do would you want too try mist it with some kind of water?
Just a tip for people that live in a newly constructed home: your house, most likely, doesnât have enough contaminants built up to affect your grows. So you donât even need a SAB or flow hood. Just do your sterile work in open air. But make sure you change your home air filters regularly and to turn off the HVAC system about an hour prior to working. Also, have good sanitation practices!
This ain't rocket science. Make it make sense. Dollar store pool noodle cut to circumference of the arm holes, then cut long ways to the center of the noodles. Place around the arm hole edges. Skin cutting Problem solved...
FYI shroomery has long advised against using lysol because it is petroleum based and is believed to potentially cause mutations. Lots of people still use it, but I think bleach or 70% ISO is a better option. Remember, there's zero chance of actually creating a sterile environment like this, you're just trying to reduce the chances of potential contamination.
Unfortunately, this is bad advice and even worse procedure. The air, wherever your room is, contains microbes of all kinds which cause contamination. Lysol is a disinfectant, not a steriliser, does not result in asceptic workspace, especially open to air movement like your open plastic box.
I got some spores and put them to agar. The problem is they were delivered early and sat in the mailbox for 2 days. It is super hot here and I'm concerned that by not knowing they were here, I unknowingly screwed up and they probably aren't viable. Golden Teacher and Koh Samui are the strains. I am just learning from you and mycotrophic and I hate to think I wasted my $. Can you tell me your thoughts?
You do an initial wipe down with a sanitizer, then coat the inside with a fine mist of water (ideally with very little soap) to trap particulates floating around in the box
Get Exclusive step by step videos, 1on1's, private chat & much moređđŒ
www.patreon.com/willymyco
All social media and only social media đđŒ
instagram.com/ttf_willy
instagram.com/official_ttf_willy
twitter.com/willy_myco
Music By: D'rok The Menace
TTF submitted and used photos S/O's
instagram.com/mushlovedelivery
instagram.com/texastoadstools
i love low tech diy for those of us that aren't planning on going big. thx!
I use oven tek for my agar pours. Preheat to 250, crack it open and place a wire rack over the opening. Do your pours over the rack, good to go. Learned it from PGT.
Thanks for the video Willy, good info for sure
I do mycology in the closet. No air current. I have pretty good results. With safe food handling practices. Bleach water spray bottle is my go to, sanitation for pennies, millet bird seed it the grain of choice you can get like a 30# bag for like $14, you can find damaged bags for like $3 at lowes and the depot stores.
Teach me!
Would you like to start a channel and teach us lol
I've had great success(10 agar transfers and 4 grain transfers with no contamination) by using this sab method and I am happy you posted about it because everyone thinks im crazy for not cutting arm holes. I started doing it a few months ago because I realized during a smoke test that when I put my hand into a sab(through holes) air is sucked in and it does the same thing when you exit. The trick is you have to have the opening flush with the edge of your kitchen counter so you arm movements do not push air into the counter and into the sab. You also cannot have any airflow pointed towards the opening. I also sanitize my hands and arms every time I leave the sab even if its for a second. If I am working more than 15 min then I spray lysol into the bin. You can spray even if there is stuff in there as long as none of your plates or jars are open. I actually think this is more effective than the traditional SAB with arm holes. The armholes are great if you do not leave the sab until you are done but if you have to exit for any reason then when you re-enter you push air from outside into the sab(from air displacement) directly over your samples. If you are disciplined with your movements (always reach from below or level with your samples) and with sanitizing using the open SAB is more effective.
Hello.
How to sterilize a scalpel and an inoculation loop with an open fire in such a box? will fire create air movement or not? besides, it's flammable, because lysol contains alcohol, isn't it?
Please help, I can't figure out this issue with the sterilization of a metal instruments.
@@user-ti3nq1pf1w I use a lab grade alcohol lamp too sterilize my tools. I do not keep the lamp in the SAB. I leave a few feet of open counter next to the SAB and sterilize it with alcohol and lysol before placing my lamp right next to the sab. Sterilize your instruments then while they are still red hot transition to working in the sab. Smooth but urgent movements are key to prevent contamination. Plan and practice everything you are going to do in the sab before you do it. This technique isn't perfect and there is still a risk of contam no matter how good you are. I have since upgraded my setup to a labrats laminar flow hood. Edit: let the lysol and alcohol settle/evaporate before you light anything outside the sab. There can be fumes inside the while you work but I always extinguish candle before I spray.
I take a 10 gal aquarium, turn it on its side. Then I tape a towel to the top edge and two sides leaving the bottom with the slack of the towel.
I use my 2nd wardrobe closet no clothes, storage products, no vents= no airflow- possible contam⊠I use the top shelf and got a few of the Boomer SHROOMER inflatable monotub. It took me a few weeks to find my groove getting my grains to not turn into contam. I barely go into my closet lab space, unless Iâm working, and I take a shower and turn ac off and spray my gloves, forearms, and upper torso with 70% iso. Also I use a plastic tote that melted 8 holes the diameter of a Campbell soups can heated up red hot, and carefully apply pressure into the tote for the holes to be melted in perfect circles no drill debris of plastic shavings. Iâm still learning but I finally got it down now. Next step Iâm making my own DIY Mini laminar flow hood all for under $80
Well done Willy. Thrilled to see you beat the pricks that shut you down some time ago.
Hah! I just saw this and Iâve been using my box exactly like this but I added an extra little safeguard. I added a cut piece of clear plastic tarp to cover the majority of the front part with a slit cut 1/2 way up so my arms can go inside freely.
It's been so long since I seen a Willy!
Those other "content scalpers" just don't "MEASURE UP".
Good to see you're still kicking it Myco!
I always wanted to learn more about Mycology from a mortal kombat character, lol
đźđđ Thank you for doing the Lord's work
So many people need to see things done to 'learn'. Reading about it is one thing.. seeing it with your explanation, I'm sure, helps a lot of people. Rock on!
facts
For your SAB holes all you have to do is put duct tape around the holes or glue a plastic tube around the holes to help your arms
instead of cutting the holes find a metal can about the size of your arms, heat it up on the stove and burn the holes through. leaves a much smoother finish and a perfect circle with less chance of cracking the tub.
Willy is a real hero so much knowledge thank you Brother.
he keeps it going
I've only done it once right in front of the hot oven, advised by midwest. I got the kit from them. Have you heard of of oven method? After seeing this it does seem better than the oven method. I am going to do it my next go around.
Anyway, you are a wealth of knowledge. I hope to be on the next zoom.
headed over to P to see the next video now.
Yup I use a c-clap to keep rack secure. I learnt from Home Mycology. Taping a clear dry cleaning bag to small hepa fan works really nice as well. Makes huge sterile area to work
@@PageG111 thanks!
Always bringing the good content in, thank you Willy's World! Don't stop making videos
I saw a trick where someone put their oven on very low and had a wire trey reaching out over a slightly open oven. The theory was that the rising heat created a somewhat laminar flow upwards and he poured his agar that way with the dishes on the wire trey. I have a sab so I don't need to do that. But it sounds like a good idea for broke boy tech. Haha. It would be cool to see some comparative results with these different techniques to see if one has less contam rates then the other.
I've done that oven tech 4 times now making plates and slants and it works surprisingly well. I usually pour 4 cups of agar each time. I've been using the 2 ounce plastic condiment cups so I come out with quite a few. Probably about 50 but I usually do a fair amount of slants too which seem to have come out clean. Last pour I only have had one plastic cup with a little contamination so far. 25 cups I did of a separate syringe all came out dark brown everywhere the loop sneaked across the agar and it was fast. Second day I was throwing them away. The scary part was that I used the same loop for 4 different species one right after another. So we know that flame sterilizing does a excellent job.
Thanks! I like this, what I have done is take a similar sized bin, and wrapped shipping saran wrap around the top 2/3, leaving a wide hand port. I make it clean, but my bunsen into it for 10" and go. So far, so good.
"Cuts arms" omg that made me laugh. People with that little resolve might not make it in this hobby.
The only reason I don't like a SAB is because I'm disabled from labor jobs due to my back. No worries I got a work from home job, as not working is the worst thing for anyone.
The sab is horrible for posture and long sessions are not a viable option for me long term.
Another idea to add is Saran Wrap across the front. Leave a opening at the bottom. Easy to get your arms in to work and easy to see through. Keeps the work space a little cleaner
@happygolucky1100 Iâve used this cheap very effective method without l the hassle of buying very expensive equipment that you donât necessarily need but they are very good to have to eliminate altogether or reduce contamination
This is what I do. About 2/3 of the top of the bid with Saran. Makes it so much easier to move around and see without having a the entire face open.
Lowes sells a small plastic greenhouse for 30 bucks. You put the frame together and slide the plastic shell over the top and cut 2 arm holes in it. Works great
Jesus thatâs a great idea tbh I had about 4 from when I was going to do pans didnât even think of that nice man
I flame sterilyzed my SAB.. To much Alkohol...LOLđ€Łbut i think is pretty cleanđšâđŹ
I turn my unmodified sab upside down over the edge of my workspace and work under it
A box
Me too. Works great. A little annoying when inoculating a bunch of batches, but still works
Homemade Flowhood under $30= 20Ă20 Box Fan with quality 20Ă20 Furnace Filter taped on the front. BAM Done!
Hello, just starting out and wondering if this method still works for you and is still your go to? I tried it and got decent results but I am new and those results could be from any contam point. I switched to a SAB and seems to be about 1-2 out of 10 grain jars contam. But they did not contam on matched AGAR samples I kept. I intend to go back to Your Idea but try all over now that I have more knowledge than before. Thats why I was asking what you still think a year later. Is it worth it to use the homemade flow hood? Obvious there is way better but I mean on par with your statement.
@@kylebrowning88 Still standing strong, I just replace the filter from time to time. I even used that setup to vent my chamber after misting to promote Evaporation. Works great.
I use to grow mushrooms using the hydrogen peroxide method. Helps cut down on contam and can work in open air.
H2O2 method?
@@David_Lo_Pan adding 3% of peroxide to agar,hardwood spawn and sub. All done in open air.
few drops?
OG
Nice..
Always a good day when you see a new willy vid; Thank you man
for real
Bro looks like the nerd from breaking bad đđđ
Willy Myco is the best TV show Iâve ever watched
haha facts
Willy, show us your full grow set up.
These are some of the highest production value videos about this stuff.
you redid the old vid! its so clear, amazing bro
found your channel today and i enjoyed watching you do your thing .
Love the hoodie
I would recommend always giving the full term before using acronyms. This is pretty much the law of acronyms. Iâm new to this world and have no idea what an âSABâ is which was used at least 20xs in this video.
Where did you get that hoodie? I love it.
Thank you for all teaching brother!
Thank you! It was a gift from a TTF member that works for me. I just asked them and They bought it at Tillys
Working over a cracked oven with a rack also works nicely, shout out to Home Mycology. Have also taped open end of clear dry cleaning bag to HEPA fan. Turn on and cut arm holes in side, balloons out creating huge sterile area to work
Thanks PageG.....seems I saw that tek on another site. Yeah those "dry cleaner bags"...that cover shirts/coats/etc. are really a great idea. Thanks again Page.
Almost forgot about it....
Also I gorilla taped and cut a small cross with a razor blade into two sandwich bags over the arm holes which were just cut bigger so i didn't cut myself. This keeps my feom cutting myself and keeps air from getting in when i put my arms in and out... And i have done the same thing, but with clear industrial strength Saran wrap over the top half but tbh the alcohol lamp just worked better for me as long as you work close. You just need heat to push it all away. Also you can build a laminar for about 80-100 bucks its just the right fan behind a HEPA and enclosed, im even going to add some UV lights
brothamyco here showing some love đ»đšđšđšđšđšđš
I never made a SAB. I took a tub and just turned it on its side and sprayed isopropyl alcohol on absolutely everything I touched and transferred to/from. Keep open flames away from that environment if you follow my lead.
I've only had contam in a few agar cups. Transfers from agar to jars or jars to tubs have not had any contam. Not sure why only a few agar cups have contam and nothing else has. I suspect maybe syringes were contaminated?
Willy Motormouth
Dope video Willy, great knowledge!
I LOVE U willy
Peace Thank you for all your teachings
Put a clear plastic sheet as a curtain/drape to increase sterility!
can't wait to try this method! Can I also make agar transfers this way?
Glad to have you back, happy you are feeling better, bro! You are amongst the best teachers I've had, Thank you.
Salute, Willy đ«Ą
Thank you for your videos! Itâs very interesting, you doing amazing job! Can you do video how to get 2nd and 3d flash for small commercial farm? I just started and canât find how to do in CZcams. Not sure if I have to soak each bag for 12 hours? Itâs a lot of work, is there is a way to do without soak in them and get 2-3 flashes?
Use weather stripping and super glue for the arm holes đ
Funny how no one in the community seems to appreciate the open flame technique, which is btw the standard in academic microbiology. Typicaly it would require a bunsen burner and a source of gas, but I work with fondue fuel and the lil burner that comes along and i get pretty decent results, working in the sterility cone. I'll admit I'm using PastyWhite's no pour agar technique though.
I was using an inoculation loop to start 4 different species on agar. After about 3 days I checked them and one species I had about 25 agar cups of was growing dark brown everywhere I streaked the agar plate with the inoculation loop. No white mycelium, just dark brown. The other 3 species have come out really clean and healthy. The ones that came out brown and contaminated where the first ones I did. Did all this in the same SAB Box, same batch of agar, with the same inoculation loop. If I didn't flame sterilize the scalpel, that would happened to everything. I only flame sterilized between each species. I really think the syringe is contaminated but I made more agar cups and put the spore solution on the agar directly from the syringe just to reduce as much contact as possible just to see if it happens again. Also flame sterilized the needle so I can hopefully narrow down what caused such an aggressive contamination. It took over 80 percent of the agar surface in just 2 days. It will be interesting to see if it happens the second time.
Do you still do this and what are contam rates like? Iâve been thinking about just working close to my alcohol lamp but not a lot of info out there on this.
What about an alcohol lamp? It's cheap, easy, and you can diy
Genius. Thank you.
That hoodie though
You can make a flow hood for 40-100$. Only way to go. Either box fan with home filter taped to it. Or an eBay mounto hepa filter with an old squirrel cage and build a box. Hot glue works to seal shotty wood work đđŸgo from 5-10% contam to 0. I put it in an ice shanty to recirculate clean air
you could do this but tape a piece of clear plastic over the opening and put holes in that so it is a more flexible SAB
Thanks đ
Willy can you sterilize the other plates that didnât get used?
Is this better then making a candle circle around the working area?
Our there any cultivation classes located near or around Oakland?! Willing to pay good money for hands on education/ skills
I'll refer you to this mate where I got mine
delivered discreetly***¿¿¿
On Instagram..Âż
Healerbryan2âŠ
Thank you so much!!!
Can you just spray it with 70% alcohol instead of lysol or the bleach mixture?
This is exactly what I did a week ago on a spawn to spawn transfer! Except I was also running 2 UVC w/ ozone lights inside the box. Iâve killed contamination with these lights. A must have!!
Please be careful with uv. My homie got wicked face/neck sunburn and couldnât open his eyes for a day. He didnât know it was on under a table, was out of sight. Had a remote and usually turned off hour before entering. He was in the room just over half hour and messed him up bad. Just be safe with them
Love it
hes on it like a boss
I remember you on a diff channel im sure⊠i remember yr vids being super detailed and good a back in the day . Now energy prices are bs worldwide recn mushies would be good to get goin
Notification gang
haha
GREAT!
Brand freaking new to the mycology world, found your video and will be getting started this week. Any tips for a first timer??
Agar...right off. Don't waste time with brf cakes....start bulk ASAP
Wear a condom
This guy is not a good resource for mycology. You are better off with channels like Stipe & Cap, the Trusted Cultivator and sites such as the Shroomery.
I spray my bathroom with lysol and never had an issue even doing transfers.
Can you use an air purifier in a small work room? Of course used with a sterilized work area.
My agar plates always end up wet with clumps. What am I doing wrong
Maybe let it cool some before pouring
im still figuring stuff out, the kit i have had me innoculate jars in front of my oven at full blast with the door down and doing it right on the door it self or holding in front of it at least. does this not work for things like agar and syringe and prints i take it? just curious, as it would be the most convenient i feel.
Get it Lit Willy
What's your thoughts on using charcoal filtration system instead flow hood? Unfortunately it is setup to recirculate. I'm going to exhaust it out window so it'll be negative air pressure? I'm novice/ beginner. Thank you for teaching me new things the correct way. I found new agar plates & ready bags with contamination đ©đ seems like agar containers should snap r screw on đ€đđ
Positive flow from hepa filter
Carbon won't work for this. It does chemical filtration, removes molecular sized contaminants from the air. You need mechanical filtration, like a HEPA filter, to remove the larger bacteria/fungus spores from the air.
Is this as good as a still air box for purposes like innoculation, agar work, and transfers?
cracked ovens and open boxes..... shit you really figured this all outđ
SAB is not a clean space and is not free of contaminants. Wth are you talking about homey.
And it's definitely not Sterile.
So i got a question. So say i get some plates and they dont germinate because the agar was too dry when you inoculate what should you do would you want too try mist it with some kind of water?
How long does it usually take for agar to solidify at room temp?
Why is condensation bad on agar lids??? I'm going to try agar soon, thanks
But isnât it being contaminated with the lid off while youâre pouring?
Just a tip for people that live in a newly constructed home: your house, most likely, doesnât have enough contaminants built up to affect your grows. So you donât even need a SAB or flow hood. Just do your sterile work in open air. But make sure you change your home air filters regularly and to turn off the HVAC system about an hour prior to working. Also, have good sanitation practices!
đ
This ain't rocket science. Make it make sense. Dollar store pool noodle cut to circumference of the arm holes, then cut long ways to the center of the noodles. Place around the arm hole edges. Skin cutting Problem solved...
FYI shroomery has long advised against using lysol because it is petroleum based and is believed to potentially cause mutations. Lots of people still use it, but I think bleach or 70% ISO is a better option. Remember, there's zero chance of actually creating a sterile environment like this, you're just trying to reduce the chances of potential contamination.
Unfortunately, this is bad advice and even worse procedure. The air, wherever your room is, contains microbes of all kinds which cause contamination. Lysol is a disinfectant, not a steriliser, does not result in asceptic workspace, especially open to air movement like your open plastic box.
Willy, what tier do I need to sub to on your Patreon to get access to the library??
Hey I got those same gloves⊠lolđđđ
I got some spores and put them to agar. The problem is they were delivered early and sat in the mailbox for 2 days. It is super hot here and I'm concerned that by not knowing they were here, I unknowingly screwed up and they probably aren't viable. Golden Teacher and Koh Samui are the strains. I am just learning from you and mycotrophic and I hate to think I wasted my $. Can you tell me your thoughts?
Did the spores ever germinate?
Update.
updates?
I †Willy
Do you do consulting work? Oakland is wide open I may make an investment in the next 12 mo.
Do you need growers
I grow
I do reply if you want details
i use my left over plates for spore prints
is there any evidence of this guy actually growing mushrooms
Bro.... cut your videos down. You repeat yourself 20 times at the beginning of every video
Great videos butTake that Filter off you face man! I'm sure I'm not the only one that feels that way.
So you can use alcohol after using bleach in there?
You do an initial wipe down with a sanitizer, then coat the inside with a fine mist of water (ideally with very little soap) to trap particulates floating around in the box
Question: Why can't you sterilize the agar discs and use them again?
This is 2mo late but I believe itâs because those containers canât go into the pressure cooker. If they were PP5, you should reuse them.
This dude is like peak intelligence bo from super store. He needs to airhorn outro. Bher bher bherrr!
600$ pro guard air filter system. Cleans air with uvc light and carbon filter. Your welcome.
This should be filed under satire.
You need to add wide sunglasses, we KNOW who you are, the MASK...wait...are these videos the same ones i saw 3 years ago..you know Pre -Corona?
Put ur damn mask on
đ