Eukaryotic Translation Animation

Eukaryotic translation is the biological process by which messenger RNA is translated into proteins in eukaryotes. It consists of four phases: initiation, elongation, termination, and recycling.
Initiation of translation usually involves the interaction of certain key proteins, the initiation factors, with a special tag bound to the 5'-end of an mRNA molecule, the 5' cap, as well as with the 5' UTR. These proteins bind the small (40S) ribosomal subunit and hold the mRNA in place. eIF3 is associated with the 40S ribosomal subunit and plays a role in keeping the large (60S) ribosomal subunit from prematurely binding. eIF3 also interacts with the eIF4F complex, which consists of three other initiation factors: eIF4A, eIF4E, and eIF4G. eIF4G is a scaffolding protein that directly associates with both eIF3 and the other two components. eIF4E is the cap-binding protein. Binding of the cap by eIF4E is often considered the rate-limiting step of cap-dependent initiation, and the concentration of eIF4E is a regulatory nexus of translational control. Certain viruses cleave a portion of eIF4G that binds eIF4E, thus preventing cap-dependent translation to hijack the host machinery in favor of the viral (cap-independent) messages. eIF4A is an ATP-dependent RNA helicase that aids the ribosome by resolving certain secondary structures formed along the mRNA transcript.[1] The poly(A)-binding protein (PABP) also associates with the eIF4F complex via eIF4G, and binds the poly-A tail of most eukaryotic mRNA molecules. This protein has been implicated in playing a role in circularization of the mRNA during translation.[2][3] This 43S preinitiation complex (43S PIC) accompanied by the protein factors moves along the mRNA chain toward its 3'-end, in a process known as 'scanning', to reach the start codon (typically AUG). In eukaryotes and archaea, the amino acid encoded by the start codon is methionine. The Met-charged initiator tRNA (Met-tRNAiMet) is brought to the P-site of the small ribosomal subunit by eukaryotic initiation factor 2 (eIF2). It hydrolyzes GTP, and signals for the dissociation of several factors from the small ribosomal subunit, eventually leading to the association of the large subunit (or the 60S subunit). The complete ribosome (80S) then commences translation elongation.
Termination of elongation depends on eukaryotic release factors. The process is similar to that of prokaryotic termination, but unlike prokaryotic termination, there is a universal release factor, eRF1, that recognizes all three stop codons. Upon termination, the ribosome is disassembled and the completed polypeptide is released.

[@annabelpeter8163]

I think I speak for most of us that watched the transcription series and translation series, YOU ARE AMAZING THANK YOU SO MUCH THANK YOU SO MUCH THANK YOU SO MUCH THANK YOU SO MUCH

[@hussainbiology]

thanks for appreciation..Glad it helps ✌️

[@hanielnkadi3040]

I needed this in my life. Thanks a lot.

[@MedicalBlackBook]

I love your explanation seriously I was waiting for this video , you have made genetics super easy I wish I had such a video when I was doing 2nd year genetics literally I had to imagine so much to make a story in which geneticw made sense ..... seriously I can't thank you enough .Bless you

[@hussainbiology]

thanks for appreciation..Glad to know that it helps ✌️

[@MedicalBlackBook]

@@hussainbiology verrrryyyyy much .thankyouuu sooo much (again) 😊

[@areejakhtar4212]

Really amazing. Explained wonderfully! thanks!

[@hussainbiology]

thanks for appreciation.. Glad it helps...

[@brendanmark4372]

I'm a student in my final year of high school and we're learning an abridged version of this. But your explanation was really easy to understand with the animation! Thanks for the video :)

[@hussainbiology]

thanks Brendan for appreciation..Glad to know that it helps ✌️

[@ammarahmehak9986]

ossum explanation.
great work keep it up👍

[@Maurya_d59]

You're just amazing brother ❤️❤️❤️

[@shwetapathak4313]

Very informative, deep knowledge in very short time,,well explained and executed...

[@geraldineezeka5862]

This was SO helpful!

[@douniahachid3681]

I love this so so so so so much!!!!!!! Thank you infinitely

[@hussainbiology]

thanks for appreciation ✌️Glad to know that it helps ✌️

[@anshikarohilla442]

Thank you so much. Great work, well done.

[@hussainbiology]

thanks for appreciation..Glad to know that it helps ✌️

[@anshikarohilla442]

@@hussainbiology yes it worked a lot. Love from India

[@elitefreerun8778]

1 hr of lecture explained in 4 min ❤ thanks a lot

[@hussainbiology]

thanks for appreciation..Glad it helps ✌️

[@SamirKhan-ee6bd]

To the point Explanation. I like your explaination and Animation Sir

[@hussainbiology]

thanks for appreciation..

[@mb010nirupamabhimani4]

Sir, apke sare video boht achhi trh se smjay hue hai aur bohat helpful hai.
Thank you for these amazing videos.

[@hussainbiology]

thanks for appreciation... Glad to know that it helps..

[@katherinruthtacoquispe7196]

Muchas gracias, bien explicado

[@kalkidanberhanu7708]

Very concise video. Thanksss ;)

[@okpolihenry983]

Watching this for my exam tomorrow in Molecular biology

[@hussainbiology]

All the best buddy.. Do well

[@buddisudarsanam5309]

Me too

[@shaanaavdaniel3915]

thank you!

[@Curlgirlmoves]

Thank you it's really helpful thank you 🙏

[@BasicScienceSeries]

Good animation with detailed explanation. Good work.

[@hussainbiology]

thanks for appreciation

[@senthimca]

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[@senthimca]

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[@zakiasultana1901]

It's very clear to me now..thanks a lot Sir 😊

[@hussainbiology]

most welcome

[@Hayatkhan-by6bd]

Thank u soo mch sir this short video is very helpful...❤❤...it clear my concept fully in very short time...thanks for uploading😍

[@hussainbiology]

thanks Hayat for appreciation..Glad to know that it helps ✌️

[@yukimizori9940]

Thank you ☺️

[@bunnylovers9877]

Nice explaination , waiting for the upload of next video

[@hussainbiology]

thanks for appreciation..glad to know that it helps ✌️

[@Shadow-bx8ri]

Precise. Precious. Perfect......thanks a lot Hussain Biology for making this video. It made my misconceptions cleared. ❤️❤️

[@hussainbiology]

thanks for appreciation..Glad to know that it helps ✌️

[@Shadow-bx8ri]

@@hussainbiology And just one more request sir....while demonstrating something try to say slowly, some of the pronounciation were difficult to understand. No offense......Kudos to your efforts..❤️❤️

[@hussainbiology]

@@Shadow-bx8ri i accept that.. ...Next time i will be slower

[@Shadow-bx8ri]

@@hussainbiology That's fine, sir......❤️❤️

[@user-lx6lq7xb7c]

thank you !!!!!!!!!!🙏🙏🙏🙏

[@biologicalresearch5836]

I appreciate your work.upload more videos

[@hussainbiology]

thanks for appreciation..Glad to know that it helps ✌️

[@angrybird1432]

Thanks a lot 🙏🙏🙏🙏

[@aparnaaa273]

Thank you so so much🙏🏻🙏🏻

[@hussainbiology]

most welcome 🙂

[@mithunkarthick5795]

Very useful fr NET exam🤟👍 tq

[@hussainbiology]

Welcome 👍

[@heerchaudhry2496]

omg i luv thix video most informative thankooo for making thix video thankoooo🤩🤩🤩🤩😍😍😍😍

[@hussainbiology]

thanks for appreciation..Glad it helps

[@shimam8778]

Wow only in 4 minutes u completed it with so much details. Keep posting more videos, it will be really helpful for us. Plz keep posting. ❤️❤️❤️

[@hussainbiology]

thanks for appreciation.. Glad to know that it helps ✌️

[@ammarahchaudhary2820]

Nice explanation.

[@hussainbiology]

thanks

[@Shikhasharmaaaa]

Yes thanks 🎉

[@shahabkaram2439]

You are awesome bro😍

[@hussainbiology]

thanks for appreciation

[@saiprasanna8893]

Thanks a lot sir... 🙏

[@hussainbiology]

most welcome

[@mahdiomidvar564]

you are great.

[@hussainbiology]

✌️

[@kashmirapradhan6542]

Amazing ..... 👏 work
I wonder which animation app you use
As I want to make videos such as yours

[@hussainbiology]

thanks Kashmira for appreciation..Glad u like my stuff....i use Final Cut Pro X

[@kashmirapradhan6542]

@@hussainbiology thankyou sir

[@hussainbiology]

@@kashmirapradhan6542 most welcome

[@lucygomango899]

Tq so much sir🙏🙏🙏💐💐💐

[@hussainbiology]

You are welcome

[@neetnet5033]

Ur all video awesome for last min revision great job😇

[@hussainbiology]

thanks for appreciation... All the best

[@sajinasasi2603]

Thanx sir

[@hussainbiology]

You are welcome ✌️

[@swapnalikamble5753]

nice animation ❤️but I have one daubt regarding termination of translatio ....In termination, is there only one relase factor used for recognise all three stop codons ?

[@shimam8778]

Yes eRF1 can recognise all the three stop codons.

[@soumyakantighosh7807]

❤️❤️

[@tanu6464]

😊😊😊😊😊😊waooo

[@pragatiuniyal8546]

Sir is there any 2nd vedio explaining elongation and termination process also....?

[@hussainbiology]

Apologies....I have all summed up here bcz this elongation and termination are simple and brief, so couldn't make seperate video...

[@ammaramehak9473]

sir plzzzzzzz upload next video as early as possible.
sir plzzzzzzzzzzzzzzz reply the Ans of my question.

[@hussainbiology]

I am working on that and InshaAllah will be putting in the form of video soon

[@spmurugansk2127]

Is In eukaryotes 9 initiation factor present or not

[@purinji_padipom]

I am grade 12 . I just wanted to know how actually translation happens and woah its complex

[@gianniskirittopoulos748]

I think that the eIF5B/GTP brings 60 S rRNP at 48 S P.I.C. taking energy by hydrolysis of P-a-bond of eIF5B ----> eIF5B/GDP (OFF) PS: In my opinion.

[@tanu6464]

☺☺

[@ammarahchaudhary2820]

Sir plzzzzzz upload next videos plzzzzzzzzzzźzzzzzzzzz

[@hussainbiology]

Published

[@danartic5217]

I can barely understand what he's saying sometimes...

[@vidugoyal70]

great work bro : whats your email address, i would like to send you a mail regarding some project.

[@hussainbiology]

[e-mail]

[@reshiishfaq1964]

Brother apna no send karo me from Kashmir

[@jabeenmanzoor6427]

I think you are from kashmir for sure

[@hussainbiology]

Yes From Kashmir ☺️

[@yourstrulybooks]

because of accent...I was soooooooo happy..someone from kashmir is teaching on CZcams..I will watch all your CZcams videos...may ALLAH bless you... @hussain biology...

[@hussainbiology]

thanks for appreciation..Glad to know that it helps ✌️

[@biologicalresearch5836]

O kashurr chuukk.
Kashir katee chukk rozaan ??

[@sparkeray4112]

GRADE 12 ME : WAT IS THIS...

[@hussainbiology]

😁

[@user-xo4cp7ym6b]

Pronounciation poor