Eukaryotic Transcription

Transcription in Eukaryotes, with mnemonics.
The enzyme required for the process of transcription is the RNA polymerase. The prokaryotes have only one RNA polymerase enzyme while the eukaryotes have three RNA polymerase enzyme. RNA polymease 2 is a major polymerase enzyme involved in (transcriotion of mRNA in the eukaryotes) the RNA pol 2 along with other proteins known as the transcription factors are required for the initiation of transcription. because there are many transcription factors involved in eukaryotic transcription, its easy to memorise them with the help of mnemonics. once RNA polymerase has initiated transcription it shifts into elongation phase. the transcription factors that helps in elongation are called elongation factors. And finally when the RNA polymerase reaches the end of the gene termination of transcription is carried out by termination factors.

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[@saramalik5440]

TFIIB joins to the upstream of promoter at BRE region not downstream .Also TBP is a subunit of TFIID only, it comprises of two subunits , TATA binding protein (TBP) and TAFs ( TATA associated factors)

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[@saramalik5440]

TFIIB joins upstream of PIC complex, not downstream and it’s not TFIIB sole acting here, it’s a ternary complex with base specific interactions (, not non-specific as you incorrectly stated,)with the major groove upstream (to BRE). It’s this assymetric binding of TFIIB to TBP-TATA which induces assymetry in the rest of assembly of PIC and unidirectional transcription that results.

[@saramalik5440]

Also TFIIB majorly stabilises melted DNA somewhat akin to row 3/4 linker in bacterial case until RNA-DNA hybrid take over. Kinda of molecular mimic to RNA

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Thanks a lot, It was difficult for me to study transcription in Eukaryotes.

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[@soniroy9038]

I am studying one paper where they did a Chip assay in osta promoter human and mouse. It is really surprised me how RNA Pol II bands are present in FXRE (more than 1.4kb upstream). The author showed a RNA polymerase ii band in gel picture at FXRE site (which is far ahead up of core promoter). That is not possible. It is impossible to get a band there. RNA pol ii moves to Gene body not backward in Promoter.
I will appreciate your feedback.

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TFIIE and TFIIH don't bind to downstream of pol, they bind to upstream of pol, where H can phosphorylate CTD.

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